Alternate Names	Interleukin-2 receptor subunit beta,Il2rb,IL-2 receptor subunit beta, IL-2R subunit beta,IL-2RB,High affinity IL-2 receptor subunit beta,p70-75,CD122
Swissprot	P16297
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245. 3. Suzuki H, et al. 1995. Science 268:1472. 4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.

Product Details

Form	Liquid
Clone No.	5H4(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	Elab Fluor^® 647 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833M]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® 647
	Elab Fluor^® 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD122 Antibody[5H4] E-AB-F10290	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD122 Antibody[5H4] E-AB-F1029A	25μg, 100μg	Check More
Biotin Anti-Mouse CD122 Antibody[5H4] E-AB-F1029B	25μg, 100μg	Check More
FITC Anti-Mouse CD122 Antibody[5H4] E-AB-F1029C	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD122 Antibody[5H4] E-AB-F1029D	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD122 Antibody[5H4]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD122 Antibody[5H4]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD122 Antibody[5H4]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD122 Antibody[5H4] E-AB-F1029I	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD122 Antibody[5H4]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD122 Antibody[5H4]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD122 Antibody[5H4] E-AB-F1029UC	25μg, 100μg	Check More
PE Anti-Mouse CD122 Antibody[5H4] E-AB-F1029UD	25μg, 100μg	Check More
APC Anti-Mouse CD122 Antibody[5H4]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD122 Antibody[5H4]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD122 Antibody[5H4]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD122 Antibody[5H4] E-AB-F1029UI	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD122 Antibody[5H4]	25μg, 100μg	Check More

Citations

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