Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Elab Fluor® 647 Anti-Mouse CD45.2 Antibody[104.2]
Cat.No.:E-AB-F1122UM
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Alternate Names | Ly-5.2, LCA |
References |
1. Suzuki K, et al. 2000. Immunity 13:691.
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Form | Liquid |
Clone No. | 104.2(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG2a, κ |
Isotype Control | Elab Fluor® 647 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09803M] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Elab Fluor® 647 |
Elab Fluor® 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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