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Elab Fluor® Violet 450 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114]

Uniprot : P14483,O78196
  • Cat.No.:E-AB-F0990UQ

  • Applications: FCM
  • Isotype: Rat IgG2b, κ
  • Host: Rat

To Purchase E-AB-F0990UQ

Size:
  • 25μg
  • 100μg
Price: $80
Qty:

Antigen Details

Alternate Names H2-Ab1/Eb1,Major histocompatibility protein class II beta chain,MHC class II H2-IA-beta-psi,I-E beta MHC class II,MHC class II
Swissprot P14483,O78196
Gene ID 14961,14969
References
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Product Details

Form Liquid
Clone No. M5/114(See Other Available Formats)
Host Rat
Isotype Rat IgG2b, κ
Isotype Control Elab Fluor® Violet 450 Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843Q]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation Elab Fluor® Violet 450
Elab Fluor® Violet 450 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450 nm (e.g., a 450/45 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F09900 50μg, 500μg, 1mg Check More
Purified Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990A 25μg, 100μg Check More
Biotin Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990B 25μg, 100μg Check More
FITC Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990C 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990D 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990E 50Tests, 100Tests, 100Tests×2 Check More
PerCP Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990F 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990G 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990H 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990I 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990J 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990UC 25μg, 100μg Check More
PE Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990UD 25μg, 100μg Check More
APC Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990UE 25μg, 100μg Check More
PerCP Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990UF 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990UG 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990UH 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990UI 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] E-AB-F0990UJ 25μg, 100μg Check More
Elab Fluor® 488 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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