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ENO1 Polyclonal Antibody

Cat:E-AB-40064
Manual MSDS
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Price: $ 380

Price: $ 230

Price: $ 125

Price: $ 65

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC
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Product Details
Verified Samples Verified Samples in WB:Hela
Verified Samples in IHC:Rat liver,Mouse brain
Dilution

WB 1:500-1:1000, IHC 1:50-1:100

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant Human Alpha-enolase protein
Abbre ENO1
Synonyms 2 phospho D glycerate hydro lyase;2-phospho-D-glycerate hydro-lyase;Alpha enolase;Alpha enolase like 1;Alpha-enolase;C myc promoter binding protein;C-myc promoter-binding protein;EC 4.2.1.11;eno1;ENO1L1;ENOA;Enolase 1 (alpha);Enolase 1 (alpha) like 1;Enolase 1;Enolase alpha;MBP 1;MBP-1;MBP1;MBPB1;MPB 1;MPB-1;MPB1;MYC promoter binding protein 1;NNE;Non neural enolase;Non-neural enolase;Phosphopyruvate hydratase;Plasminogen binding protein;Plasminogen-binding protein;PPH;Tau crystallin
Swissprot
Calculated MW 47kDa
Observed MW 47kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nucleus and Cytoplasm. Cell membrane. Cytoplasm>myofibril>sarcomere>M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Concentration 2 mg/mL
Buffer PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.
Purification Method Affinity purification
Research Areas Cancer; Epigenetics and Nuclear Signaling; Metabolism; Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate.Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells .And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation.