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EPI(Epinephrine/Adrenaline) ELISA Kit

  • Cat.No.:E-EL-0045

  • Reactivity: Universal

To Purchase E-EL-0045

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes EPI in samples. No significant cross-reactivity or interference between EPI and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of EPI concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with EPI. During the reaction, EPI in the sample or standard competes with a fixed amount of EPI on the solid phase supporter for sites on the Biotinylated Detection Ab specific to EPI. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EPI in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level EPI were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level EPI were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 96.50 321.00 901.00 88.20 307.60 931.00
Standard deviation 6.30 13.50 36.00 4.90 13.50 37.20
CV (%) 6.53 4.21 4.00 5.56 4.39 4.00

Recovery

The recovery of EPI spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-101 93
EDTA plasma(n=8) 95-109 101
Cell culture media(n=8) 89-100 95

Linearity

Samples were spiked with high concentrations of EPI and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsEpinephrine, adrenaline, adrenalin, 4, 5-β-trihydroxy-N-methylphenethylamine

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of the International Society of Sports Nutrition (2020) IF: 5.068
    Caffeine supplementation induces higher IL-6 and IL-10 plasma levels in response to a treadmill exercise test

    DOI: 10.1186/s12970-020-00375-4

    PMID: 32907591

    Sample: Plasma
  2. Mediators Of Inflammation (2015) IF: 3.232
    Intranasal Dexmedetomidine On Stress Hormones, Inflammatory Markers, And Postoperative Analgesia After Functional Endoscopic Sinus Surgery.

    DOI: 10.1155/2015/939431

    PMID: 26199465

    Sample: Serum
  3. American Journal of Hypertension (2016) IF: 3.541
    A Novel Swine Model Of Spontaneous Hypertension With Sympathetic Hyperactivity Responds Well To Renal Denervation.

    DOI: 10.1093/ajh/hpv066

    PMID: 25958303

    Sample: Plasma,Urine
  4. PloS one (2018) IF: 2.766
    Selective ablation of the ligament of Marshall reduces ischemia and reperfusion-induced ventricular arrhythmias

    DOI: 10.1371/journal.pone.0203083

    PMID: 30153281

    Sample: Serum
  5. Turkish Journal of Science (2019)
    The Effect of Peripheral Adropin Application on Hypothalamic Pituitary Adrenal Axis in Rats

    PMID: 99999999

    Sample: Tissue homogenate
  6. Journal of Pharmaceutical Sciences and Research (2019)
    Effect of chronic toxoplasmosis on levels of some neurotransmitters (Dopamine, Adrenaline, and Noradrenaline) in human Serum

    PMID: 99999999

    Sample: Serum
  7. Journal of Obstetrics and Gynaecology (2018) IF: 0.629
    Stress response to ovariohysterectomy in rabbits: role of anaesthesia and surgery.

    DOI: 10.1080/01443615.2017.1400523

    PMID: 29519176

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