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EPI(Epinephrine/Adrenaline) ELISA Kit

  • Cat.No.:E-EL-0045

  • Reactivity: Universal

To Purchase E-EL-0045

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes EPI in samples. No significant cross-reactivity or interference between EPI and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of EPI concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with EPI. During the reaction, EPI in the sample or standard competes with a fixed amount of EPI on the solid phase supporter for sites on the Biotinylated Detection Ab specific to EPI. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EPI in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level EPI were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level EPI were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 96.50 321.00 901.00 88.20 307.60 931.00
Standard deviation 6.30 13.50 36.00 4.90 13.50 37.20
CV (%) 6.53 4.21 4.00 5.56 4.39 4.00


The recovery of EPI spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-101 93
EDTA plasma(n=8) 95-109 101
Cell culture media(n=8) 89-100 95


Samples were spiked with high concentrations of EPI and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsEpinephrine, adrenaline, adrenalin, 4, 5-β-trihydroxy-N-methylphenethylamine
Research AreaCell Biology, Metabolism, Epigenetics and Nuclear Signal, Signal transduction, Neuroscience, Cardiovascular, Cancer, Biochemicals

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


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    DOI: 10.1016/j.cell.2022.12.024

    PMID: 36669474

    Sample: Serum
  2. Cellular & Molecular Immunology (2023) IF: 22.096
    β-adrenoreceptor-triggered PKA activation negatively regulates the innate antiviral response

    DOI: 10.1038/s41423-022-00967-x

    PMID: 36600052

    Sample: serum
    Neuroendocrine stress hormones associated with short-term exposure to nitrogen dioxide and fine particulate matter in individuals with and without chronic obstructive pulmonary disease: A panel study in Beijing, China

    DOI: 10.1016/j.envpol.2022.119822

    PMID: 35870527

    Sample: serum
  4. Computational and Structural Biotechnology Journal (2020) IF: 6.018
    Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors

    DOI: 10.1016/j.csbj.2020.11.009

    PMID: 33304464

    Sample: blood
  5. Molecular Metabolism (2019) IF: 6.181
    Metformin prevents the pathological browning of subcutaneous white adipose tissue

    DOI: 10.1016/j.molmet.2019.08.011

    Sample: plasma
  6. Communications Biology (2022) IF: 6.548
    Maternal anxiety affects embryo implantation via impairing adrenergic receptor signaling in decidual cells

    DOI: 10.1038/s42003-022-03694-1

    PMID: 35982177

    Sample: serum,uteri
    Uncoupling protein -1 modulates anxiety-like behavior in a temperature-dependent manner

    DOI: 10.1523/JNEUROSCI.2509-21.2022

    PMID: 36194650

    Sample: serum
  8. Journal of the International Society of Sports Nutrition (2020) IF: 5.068
    Caffeine supplementation induces higher IL-6 and IL-10 plasma levels in response to a treadmill exercise test

    DOI: 10.1186/s12970-020-00375-4

    PMID: 32907591

    Sample: Plasma
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    DOI: 10.1016/

    PMID: 36067878

    Sample: brain tissue
  10. ACS Omega (2022) IF: 4.132
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    DOI: 10.1021/acsomega.2c03619

    PMID: 35967043

    Sample: serum
  11. ACTA DIABETOLOGICA (2022) IF: 4.28
    Deep transcranial magnetic stimulation in combination with skin thermography in obesity: a window on sympathetic nervous system

    DOI: 10.1007/s00592-022-01859-2

    PMID: 35174415

    Sample: blood
  12. Molecular Brain (2023) IF: 4.399
    Investigating the molecular mechanisms of delirium-like neuropsychiatric disorder induced by electromagnetic pulse based on bioinformatics analysis

    DOI: 10.1186/s13041-023-00998-z

    Sample: prefrontal cortex,hippocampus
  13. ENDOCRINE (2018) IF: 3.179
    High frequency deep transcranial magnetic stimulation acutely increases β-endorphins in obese humans

    DOI: 10.1007/s12020-018-1791-1

    Sample: blood
    Intranasal Dexmedetomidine on Stress Hormones, Inflammatory Markers, and Postoperative Analgesia after Functional Endoscopic Sinus Surgery

    DOI: 10.1155/2015/939431

    Sample: Serum
  15. PLoS One (2017) IF: 3.24
    Perivascular radiofrequency renal denervation lowers blood pressure and ameliorates cardiorenal fibrosis in spontaneously hypertensive rats

    DOI: 10.1371/journal.pone.0176888

    Sample: plasma
  16. International Journal of Environmental Research and Public Health (2021) IF: 3.39
    A Case Control Study on Serum Levels of Potential Biomarkers in Male Breast Cancer Patients

    DOI: 10.3390/ijerph18094852

    PMID: 34062830

    Sample: Serum
  17. JOURNAL OF FISH BIOLOGY (2022) IF: 2.051
    Primary stress response and biochemical profile of Labeo rohita (Hamilton, 1822) experimentally parasitized with Argulus bengalensis (Ramakrishna, 1951)

    DOI: 10.1111/jfb.15048

    PMID: 35319798

    Sample: plasma
  18. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    LianXia Formula Granule Attenuates Cardiac Sympathetic Remodeling in Rats with Myocardial Infarction via the NGF/TrKA/PI3K/AKT Signaling Pathway

    DOI: 10.1155/2021/5536406

    PMID: 34221073

    Sample: plasma
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    DOI: 10.1093/ajh/hpv066

    Sample: Plasma,Urine
  20. PLoS One (2018) IF: 2.766
    Selective ablation of the ligament of Marshall reduces ischemia and reperfusion-induced ventricular arrhythmias

    DOI: 10.1371/journal.pone.0203083

    PMID: 30153281

    Sample: Serum
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    DOI: 10.1080/01443615.2017.1400523

    Sample: Serum,Plasma
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    DOI: 10.1016/j.smallrumres.2018.08.009

    Sample: serum
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