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Excellent Chemiluminescent Substrate (ECL)Detection Kit


      Catalog number:E-IR-R307

      • 50 mL
      • 100 mL
      • 500 mL
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      Price: $50

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      Elabscience ® Excellent Chemiluminescent Substrate(ECL)Detection Kit is a horseradish peroxidase (HRP) substrate, which can be used to detect horseradish peroxidase (HRP) labeled proteins or nucleic acids, analyze protein content by Western blot or ELISA, and is compatible with film and digital development system.


      Cat. Products 50 mL 100 mL 500 mL Storage
      E-IR-R307A ECL Substrate A 25 mL 50 mL 250 mL 2~8°C
      E-IR-R307B ECL Substrate B 25 mL 50 mL 250 mL 2~8°C


      1.  Western Blot

      1)  Follow the step of Western Blotting to block the membrane and incubate the primary/secondary antibody.

      2)  Wash the membrane with TBST for 3 times, 5 min each time.

      3)  Prepare ECL Working Solution by mixing equal parts of the ECL Substrate A and ECL Substrate B.

      Note: Prepare the working solution before use, and change the pipette tips during the suction process.

      4)  Take an appropriate amount of mixed ECL Working Solution and add it to the membrane.

      5)  Adjust the exposure time according to the intensity of the luminous intensity

      2.  ELISA

      1)  Prepare Working Solution by mixing equal parts of the ECL Substrate A and ECL Substrate B.

      Note: Prepare the working solution before use, and change the pipette tips during the suction process.

      2)  Add 50~200 μL mixed ECL Working Solution to each well into the microtiter plate.

      3)  Detection within 10~60 min, better within 40~50 min.


      Store at 2~8°C for 12 months.


      1. Before using this product, the imprinting film must be thoroughly washed, otherwise it may cause the background to rise.

      2. ECL Substrate A and ECL Substrate B must be prepared before use, and change the pipette tips during the suction process.

      3. Do not expose ECL working solution to sunlight or strong light, otherwise it will cause its inactivation, and laboratory light will have little effect on the working solution.

      4. NaN3 inhibits HRP activity, NaN3 should be avoided during the experiment.


      1. Inter-Organelle Contact Sites Mediate the Intracellular Antioxidant Activity of Platinum Nanozymes: A New Perspective on Cell–Nanoparticle Interaction and Signaling
        IF: 10.383
        Journal: ACS Applied Materials & Interfaces(2023)
        DOI: 10.1021/acsami.2c22375
        Products: E-IR-R307 E-IR-R308 
      2. Construction of a self-directed replication system for label-free and real-time sensing of repair glycosylases with zero background
        IF: 9.5559
        Journal: Chemical Science(2019)
        DOI: 10.1039/C9SC04738G
        Products: E-IR-R307 
      3. Controllable Autocatalytic Cleavage-Mediated Fluorescence Recovery for Homogeneous Sensing of Alkyladenine DNA Glycosylase from Human Cancer Cells
        IF: 8.0630
        Journal: Theranostics(2019)
        DOI: 10.7150/thno.35393
        Products: E-IR-R307 
      4. Hyperbaric oxygen attenuates neuropathic pain and reverses inflammatory signaling likely via the Kindlin-1/Wnt-10a signaling pathway in the chronic pain injury model in rats
        IF: 7.277
        Journal: JOURNAL OF HEADACHE AND PAIN(2017)
        DOI: 10.1186/s10194-016-0713-y
        Products: E-IR-R307 E-IR-R308 
      5. Muscle Oxidative Stress Plays a Role in Hyperthyroidism-Linked Insulin Resistance
        IF: 7.675
        Journal: Antioxidants(2023)
        DOI: 10.3390/antiox12030592
        Products: E-IR-R307 E-IR-R308 
      6. Hepatic Insulin Resistance in Hyperthyroid Rat Liver: Vitamin E Supplementation Highlights a Possible Role of ROS
        IF: 7.675
        Journal: Antioxidants(2022)
        DOI: 10.3390/antiox11071295
        PMID: 35883786
        Products: E-IR-R307 
      7. Butyrate Improves Neuroinflammation and Mitochondrial Impairment in Cerebral Cortex and Synaptic Fraction in an Animal Model of Diet-Induced Obesity
        IF: 7.675
        Journal: Antioxidants(2022)
        DOI: 10.3390/antiox12010004
        Products: E-IR-R307 
      8. Age-Dependent Skeletal Muscle Mitochondrial Response to Short-Term Increased Dietary Fructose
        IF: 7.675
        Journal: Antioxidants(2023)
        DOI: 10.3390/antiox12020299
        Products: E-IR-R307 E-IR-R308 
      9. The Lack of STING Impairs the MHC-I Dependent Antigen Presentation and JAK/STAT Signaling in Murine Macrophages
        IF: 6.208
        DOI: 10.3390/ijms232214232
        Products: E-IR-R307 
      10. Hydroxytyrosol Prevents Doxorubicin-Induced Oxidative Stress and Apoptosis in Cardiomyocytes
        IF: 6.313
        Journal: Antioxidants(2022)
        DOI: 10.3390/antiox11061087
        PMID: 35739984
        Products: E-IR-R307 
      11. Non-covalent strategies to functionalize polymeric nanoparticles with NGR peptides for targeting breast cancer
        IF: 6.51
        DOI: 10.1016/j.ijpharm.2023.122618
        Products: E-IR-R307 E-IR-R308 
      12. PEGylated cationic nanoassemblies based on triblock copolymers to combine siRNA therapeutics with anticancer drugs
        IF: 6.843
        Journal: Biomaterials Science(2021)
        DOI: 10.1039/D1BM00909E
        PMID: 34369494
        Products: E-IR-R307 
      13. Ablation of uncoupling protein 3 affects interrelated factors leading to lipolysis and insulin resistance in visceral white adipose tissue
        IF: 5.192
        Journal: FASEB JOURNAL(2022)
        DOI: 10.1096/fj.202101816RR
        PMID: 35452152
        Products: E-IR-R307 
      14. Tilianin Reduces Apoptosis via the ERK/EGR1/BCL2L1 Pathway in Ischemia/Reperfusion-Induced Acute Kidney Injury Mice.
        IF: 5.811
        Journal: Frontiers in Pharmacology(2022)
        DOI: 10.3389/fphar.2022.862584
        Products: E-IR-R307 
      15. uL3 Mediated Nucleolar Stress Pathway as a New Mechanism of Action of Antiproliferative G-quadruplex TBA Derivatives in Colon Cancer Cells
        IF: 4.082
        Journal: Biomolecules(2020)
        DOI: 10.3390/biom10040583
        PMID: 32290083
        Products: E-IR-R307 
        Sample type: Cell lysate
        Reactivity: Human
      16. Oxidative damage and mitochondrial functionality in hearts from KO UCP3 mice housed at thermoneutrality
        IF: 4.158
        DOI: 10.1007/s13105-022-00882-9
        PMID: 35237934
        Products: E-IR-R307 
      17. Early Hepatic Oxidative Stress and Mitochondrial Changes Following Western Diet in Middle Aged Rats
        IF: 4.171
        Journal: Nutrients(2019)
        DOI: 10.3390/nu11112670
        Products: E-IR-R307 
        Sample type: liver
        Reactivity: Rat
      18. USP36 promotes tumor growth of non-small cell lung cancer via increasing KHK-A expression by regulating c-MYC–hnRNPH1/H2 axis
        IF: 4.174
        Journal: Human Cell(2022)
        DOI: 10.1007/s13577-022-00677-6
        Products: E-IR-R307 
      19. Protein Kinase A Detection in Human Urine Samples
        IF: 4.242
        Journal: Journal of Clinical Medicine(2021)
        DOI: 10.3390/jcm10184096
        PMID: 34575203
        Products: E-IR-R307 
      20. Diosmectite inhibits the interaction between SARS-CoV-2 and human enterocytes by trapping viral particles, thereby preventing NF-kappaB activation and CXCL10 secretion
        IF: 4.38
        Journal: Scientific Reports(2021)
        DOI: 10.1038/s41598-021-01217-2
        PMID: 34741071
        Products: E-IR-R307 E-IR-R308 
        Sample type: Caco-2 cells
        Reactivity: Human
      21. Semaphorin 3A attenuates the hypoxia suppression of osteogenesis in periodontal ligament stem cells
        IF: 4.419
        DOI: 10.1111/jre.12973
        PMID: 35037251
        Products: E-IR-R307 
      22. Bone-like Polymeric Composites with a Combination of Bioactive Glass and Hydroxyapatite: Simultaneous Enhancement of Mechanical Performance and Bioactivity
        IF: 4.432
        Journal: ACS Biomaterials Science & Engineering(2018)
        DOI: 10.1021/acsbiomaterials.8b01174
        Products: E-IR-R307 
      23. Ghrelin regulates the proliferation and apoptosis of high glucose-induced islet cells through the PI3K–Akt signaling pathway
        IF: 4.473
        DOI: 10.1002/cbin.11981
        Products: E-IR-R307 
      24. Mesoglycan induces keratinocyte activation by triggering syndecan‐4 pathway and the formation of the annexin A1/S100A11 complex
        IF: 4.522
        DOI: 10.1002/jcp.28618
        Products: E-IR-R307 
      25. Role of uL3 in the Crosstalk between Nucleolar Stress and Autophagy in Colon Cancer Cells
        IF: 4.556
        DOI: 10.3390/ijms21062143
        Products: E-IR-R307 
      26. Long-Term Wi-Fi Exposure From Pre-Pubertal to Adult Age on the Spermatogonia Proliferation and Protective Effects of Edible Bird’s Nest Supplementation
        IF: 4.566
        Journal: Frontiers in Physiology(2022)
        DOI: 10.3389/fphys.2022.828578
        Products: E-IR-R307 
      27. Effect of Initial Aging and High-Fat/High-Fructose Diet on Mitochondrial Bioenergetics and Oxidative Status in Rat Brain
        IF: 4.586
        Journal: MOLECULAR NEUROBIOLOGY(2019)
        DOI: 10.1007/s12035-019-1617-z
        Products: E-IR-R307 
      28. Simultaneous reinforcement and toughening of polymer/hydroxyapatite composites by constructing bone-like structure
        IF: 4.873
        DOI: 10.1016/j.compscitech.2017.08.026
        Products: E-IR-R307 E-IR-R308 
      29. Isoliquiritigenin alleviates P. gingivalis-LPS/ATP-induced pyroptosis by inhibiting NF-κB/ NLRP3/GSDMD signals in human gingival fibroblasts
        IF: 4.932
        DOI: 10.1016/j.intimp.2021.108338
        PMID: 34794890
        Products: E-IR-R307 E-IR-R308 
        Sample type: HGFs cells
        Reactivity: Human
      30. AML‑derived mesenchymal stem cells upregulate CTGF expression through the BMP pathway and induce K562‑ADM fusiform transformation and chemoresistance
        IF: 3.041
        Journal: ONCOLOGY REPORTS(2019)
        DOI: 10.3892/or.2019.7237
        Products: E-IR-R307 
      31. A low direct electrical signal attenuates oxidative stress and inflammation in septic rats
        IF: 3.24
        Journal: PLoS One(2021)
        DOI: 10.1371/journal.pone.0257177
        PMID: 34499695
        Products: E-IR-R307 
      32. Brain Nrf2 pathway, autophagy, and synaptic function proteins are modulated by a short-term fructose feeding in young and adult rats
        IF: 3.313
        DOI: 10.1080/1028415X.2018.1501532
        Products: E-IR-R307 
      33. Early Effects of a Low Fat, Fructose-Rich Diet on Liver Metabolism, Insulin Signaling, and Oxidative Stress in Young and Adult Rats
        IF: 3.394
        Journal: Frontiers in Physiology(2018)
        DOI: 10.3389/fphys.2018.00411
        Products: E-IR-R307 
      34. Salvianolic acid A promotes mitochondrial biogenesis and mitochondrial function in 3T3-L1 adipocytes through regulation of the AMPK-PGC1α signalling pathway
        IF: 3.553
        Journal: Adipocyte(2022)
        DOI: 10.1080/21623945.2022.2116790
        PMID: 36053001
        Products: E-IR-R307 
      35. Chuanzhitongluo regulates microglia polarization and inflammatory response in acute ischemic stroke
        IF: 3.715
        Journal: BRAIN RESEARCH BULLETIN(2022)
        DOI: 10.1016/j.brainresbull.2022.09.015
        PMID: 36152772
        Products: E-IR-R307 
      36. AdipoRon negatively regulates proliferation and migration of ARPE-19 human retinal pigment epithelial cells
        IF: 3.75
        Journal: PEPTIDES(2021)
        DOI: 10.1016/j.peptides.2021.170676
        PMID: 34687793
        Products: E-IR-R307 
      37. Effect of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia in vitro
        IF: 3.848
        Journal: MICROBIAL PATHOGENESIS(2022)
        DOI: 10.1016/j.micpath.2022.105964
        Products: E-IR-R307 
      38. Effect of Neiyi Prescription of QIU on autophagy and angiogenic ability of endometriosis via the PPARγ/NF-κB signaling pathway
        IF: 2.344
        DOI: 10.1007/s00404-022-06537-w
        PMID: 35366690
        Products: E-IR-R307 
      39. Salvianolic acid A promotes mitochondrial biogenesis and function via regulating the AMPK/PGC‑1α signaling pathway in HUVECs
        IF: 2.447
        Journal: Experimental and Therapeutic Medicine(2022)
        DOI: 10.3892/etm.2022.11412
        Products: E-IR-R307 
      40. MicroRNA-155 acts as a potential prognostic and diagnostic factor in patients with ankylosing spondylitis by modulating SOCS3
        IF: 2.742
        Journal: MOLECULAR BIOLOGY REPORTS(2022)
        DOI: 10.1007/s11033-022-08033-5
        Products: E-IR-R307 
      41. The expression of Cav3.1 on T-type calcium channels of rats with subarachnoid hemorrhage
        IF: 2.802
        DOI: 10.1016/j.sjbs.2020.04.010
        Products: E-IR-R307 
      42. The Role of IL-6 and TMEM100 in Lumbar Discogenic Pain and the Mechanism of the Glycine-Serine-Threonine Metabolic Axis: A Metabolomic and Molecular Biology Study
        IF: 2.832
        Journal: Journal of Pain Research(2023)
        DOI: 10.2147/JPR.S400871
        Products: E-IR-R307 E-IR-R308 
      43. CircCDC6 restrains tumor growth and glycolysis energy metabolism in colorectal cancer via regulating miR-3187-3p and downstream PRKAA2
        IF: 2.945
        DOI: 10.1007/s10863-022-09938-3
        PMID: 35438362
        Products: E-IR-R307 
      44. FABP4-mediated homocysteine-induced cholesterol accumulation in THP-1 monocyte-derived macrophages and the potential epigenetic mechanism
        IF: 1.559
        Journal: Molecular Medicine Reports(2016)
        DOI: 10.3892/mmr.2016.5315
        Products: E-IR-R307 E-IR-R308 
      45. Negative Pressure Therapy in the Regeneration of the Sciatic Nerve Using Vacuum – Assisted Closure in a Rabbit Model
        IF: 1.894
        Journal: MEDICAL SCIENCE MONITOR(2018)
        DOI: 10.12659/MSM.906696
        Products: E-IR-R307 
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