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Excellent Chemiluminescent Substrate (ECL) Kit

  • Cat.No.:E-IR-R301

  • Application: WB,ELISA

To Purchase E-IR-R301

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Price: $59
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Introduction

Excellent Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) substrate for horseradish peroxidase (HRP) enzyme that provides low picogram detection of proteins in Western blot and ELISA applications. Excellent Chemiluminescent Substrate emits light for longer and chemiluminescent signal at more than twice the intensity of other entry-level luminol-based horseradish peroxidase detection systems while also maintaining sensitivity and low background. In addition, the range of the calibration curve is much wider than other competitors. Also, the greater signal intensity is achieved using less primary and secondary antibody than typical ECL substrates. All of this for about 30% less cost than comparable products.

Method for use

ECL substrate -- An enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity of antibodies and other ELISA or Western blot probes.
Prepare Working Solution by mixing equal parts of the Substrate A and Substrate B. The Working Solution is stable for 8 hours at RT.
Please refer to the manual for the specific application method in the experiments.

Storage

2-8℃, 12 months

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Molecular Metabolism (2021) IF: 7.422
    Sodium-glucose co-transporter2 expression and inflammatory activity in diabetic atherosclerotic plaques: Effects of sodium-glucose co-transporter2 inhibitor treatment

    DOI: 10.1016/j.molmet.2021.101337

    PMID: 34500107

  2. Antioxidants (2022) IF: 7.675
    SIRT3 Modulates Endothelial Mitochondrial Redox State during Insulin Resistance

    DOI: 10.3390/antiox11081611

    PMID: 36009329

  3. EUROPEAN JOURNAL OF CELL BIOLOGY (2023) IF: 6.02
    Forskolin affects proliferation, migration and Paclitaxel-mediated cytotoxicity in Non-Small-Cell Lung Cancer cell lines via adenylyl cyclase/cAMP axis

    DOI: 10.1016/j.ejcb.2023.151292

  4. Biomedicines (2021) IF: 6.081
    Metformin Therapy Effects on the Expression of Sodium-Glucose Cotransporter 2, Leptin, and SIRT6 Levels in Pericoronary Fat Excised from Pre-Diabetic Patients with Acute Myocardial Infarction

    DOI: 10.3390/biomedicines9080904

    PMID: 34440108

  5. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Buffalo Milk Whey Activates Necroptosis and Apoptosis in a Xenograft Model of Colorectal Cancer

    DOI: 10.3390/ijms23158464

    PMID: 35955595

  6. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Breed and Feeding System Impact the Bioactive Anti-Inflammatory Properties of Bovine Milk

    DOI: 10.3390/ijms231911088

    PMID: 36232386

  7. Cancers (2021) IF: 6.639
    SIRT3 and Metabolic Reprogramming Mediate the Antiproliferative Effects of Whey in Human Colon Cancer Cells

    DOI: 10.3390/cancers13205196

    PMID: 34680344

  8. Nutrients (2022) IF: 6.706
    Milk Exosomal miR-27b Worsen Endoplasmic Reticulum Stress Mediated Colorectal Cancer Cell Death

    DOI: 10.3390/nu14235081

  9. Frontiers in Pharmacology (2022) IF: 5.811
    Integrating Gemcitabine-Based Therapy With AdipoRon Enhances Growth Inhibition in Human PDAC Cell Lines.

    DOI: 10.3389/fphar.2022.837503

  10. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Phenolic Profiles of Red Wine Relate to Vascular Endothelial Benefits Mediated by SIRT1 and SIRT6

    DOI: 10.3390/ijms22115677

    PMID: 34073604

    Sample: Cell sample
  11. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Colorectal Cancer Apoptosis Induced by Dietary δ-Valerobetaine Involves PINK1/Parkin Dependent-Mitophagy and SIRT3

    DOI: 10.3390/ijms22158117

    PMID: 34360883

  12. Biomolecules (2020) IF: 4.082
    uL3 Mediated Nucleolar Stress Pathway as a New Mechanism of Action of Antiproliferative G-quadruplex TBA Derivatives in Colon Cancer Cells

    DOI: 10.3390/biom10040583

    PMID: 32290083

    Sample: Cell lysate
  13. FEBS LETTERS (2022) IF: 4.124
    Diet-derived ergothioneine induces necroptosis in colorectal cancer cells by activating the SIRT3/MLKL pathway

    DOI: 10.1002/1873-3468.14310

  14. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556
    Adipose Tissue and Brain Metabolic Responses to Western Diet—Is There a Similarity between the Two?

    DOI: 10.3390/ijms21030786

  15. International Journal of Environmental Research and Public Health (2021) IF: 3.39
    Assessment of Unstimulated Whole Salivary Tumor Necrosis Factor Alpha (TNF-α) and Cellular Micronuclei Levels in Snuff (Naswar) Users and Non-Users for Early Diagnosis of Oral Squamous Cell Carcinoma

    DOI: 10.3390/ijerph18147230

    PMID: 34299679

    Sample: saliva
  16. Molecular Medicine Reports (2020) IF: 2.1
    Panax notoginseng Saponins protect auditory cells against cisplatin‑induced ototoxicity by inducing the AKT/Nrf2 signaling‑mediated redox pathway

    DOI: 10.3892/mmr.2020.11390

  17. Molecular Medicine Reports (2015) IF: 1.554
    Effect of rutin on spinal cord injury through inhibition of the expression of MIP-2 and activation of MMP-9, and downregulation of Akt phosphorylation

    DOI: 10.3892/mmr.2015.4357

    Sample: Cerebrospinal Fluid
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