Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> FITC Anti-Human CD54 Antibody[15.2] E-AB-F1043C
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|Alternate Names||Intercellular adhesion molecule 1,Icam1,MALA-2,MyD10,CD54,Icam-1|
1. Voraberger G, et al. 1991. J. Immunol. 147:2777.
2. Staunton DE, et al. 1988. Cell 52:925.
3. Greve JM, et al. 1989. Cell 56:839.
|Clone No.||15.2(See Other Available Formats)|
|Isotype||Mouse IgG1, κ|
|Isotype Control||FITC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792C]|
|Application||FCM (See the Application Description)|
|Storage Buffer||Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.|
|Recommended Use||Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.|
|Shipping||Biological ice pack at 4℃|
|Stability & Storage||Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
|FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).|
How to select the appropriate detection channel through the spectrogram?
|CD54 is a 85-110 kD type I transmembrane protein also known as ICAM-1. It is expressed on activated endothelial cells, high endothelial venules, T and B cells, monocytes/macrophages, granulocytes, and dendritic cells. The expression of ICAM-1 can be released from the cell surface. CD54 plays a role in cellular adhesion and is involved in inflammation and leukocyte extravasation. CD54 has also been shown to be the major cellular receptor for rhinovirus. ICAM-1 binds to CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) as well as hyaluronan and fibrinogen.|
|Related Protocols||Staining Cell Surface Targets for Flow Cytometry|
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