Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> FITC Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] E-AB-F1016C
Cat.No.:E-AB-F1016C
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Alternate Names | Leukocyte surface antigen CD47,Cd47,Integrin-associated protein,IAP |
Swissprot | Q08722,Q61735,P97829 |
References |
1. Gresham HD, Dale BM, Potter JW, et al. J Exp Med. 2000; 191(3):515-528.
2. Jiang P, Lagenaur CF, Narayanan V. J Biol Chem. 1999; 274(2):559-562. 3. Lindberg FP, Bullard DC, Caver TE, Gresham HD, Beaudet AL, Brown EJ. Science. 1996; 274(5288):795-798. 4. Lindberg FP, Gresham HD, Schwarz E, Brown EJ. . J Cell Biol. 1993; 123(2):485-496. 5. Mi ZP, Jiang P, Weng WL, Lindberg FP, Narayanan V, Lagenaur CF. J Comp Neurol. 2000; 416(3):335-344. 6. Oldenborg PA, Zheleznyak A, Fang YF, Lagenaur CF, Gresham HD, Lindberg FP. Science. 2000; 288(5473):2051-2054. 7. Reinhold MI, Lindberg FP, Plas D, Reynolds S, Peters MG, Brown EJ. J Cell Sci. 1995 November; 108(Pt 11):3419-3425. |
Form | Liquid |
Clone No. | MIAP410(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG1, κ |
Isotype Control | FITC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792C] |
Reactivity | Human,Mouse,Rat |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | FITC |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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