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Cat.No.:E-AB-F1179UC
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Alternate Names | Cd24a, Ly-52,HAS,Nectadrin,R13-Ag,X62 heat stable antigen |
Swissprot | P24807 |
References |
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Aigner S, et al. 1997. Blood 89:3385. 3. Hough MR, et al. 1996. J. Immunol. 156:479. 4. Liu Y, et al. 1992. J. Exp. Med. 175:437. |
Form | Liquid |
Clone No. | M1/69 |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | FITC Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843C] |
Reactivity | Mouse |
Application | FCM |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | FITC |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CD24 is a 35-45 kD protein also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |