Alternate Names	Pecam, Pecam-1,PECAM-1,CD31
Swissprot	Q08481
References	1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. DeLisser HM, et al. 1994. Immunol. Today 15:490. 3. Newman PJ, et al. 1990. Science 247:1219.

Product Details

Form	Liquid
Clone No.	390(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	FITC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832C]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	FITC
	FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Biotin Anti-Mouse CD31 Antibody[390]	25μg, 100μg	Check More
PE Anti-Mouse CD31 Antibody[390]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD31 Antibody[390]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD31 Antibody[390]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD31 Antibody[390]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD31 Antibody[390]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD31 Antibody[390]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD31 Antibody[390]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD31 Antibody[390]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD31 Antibody[390]	25μg, 100μg	Check More
PE Anti-Mouse CD31 Antibody[390]	25μg, 100μg	Check More
APC Anti-Mouse CD31 Antibody[390]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD31 Antibody[390]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD31 Antibody[390]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD31 Antibody[390]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD31 Antibody[390]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD31 Antibody[390]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD31 Antibody[390]	25μg, 100μg	Check More

Citations

FASEB JOURNAL (2022) IF: 5.834
The fates of different types of adipose tissue after transplantation in mice

DOI: 10.1096/fj.202200408R

PMID: 36004579
Sample: P3 cell

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