Alternate Names	AIM,CLEC2C,GP32/28,MLR-3,VEA,EA1
Swissprot	P37217
Gene ID	12515
References	1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Testi R, et al. 1994. Immunol. Today 15:479. 3. Moretta A, et al. 1991. J. Exp. Med. 174:1393. 4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Product Details

Form	Liquid
Clone No.	H1.2F3(See Other Available Formats)
Host	Armenian Hamster
Isotype	Armenian Hamster IgG
Isotype Control	FITC Armenian Hamster IgG Isotype Control[PIP] [Product E-AB-F09853C]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	FITC
	FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
FITC Anti-Mouse CD69 Antibody[H1.2F3]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD69 Antibody[H1.2F3]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD69 Antibody[H1.2F3]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD69 Antibody[H1.2F3]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD69 Antibody[H1.2F3]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD69 Antibody[H1.2F3]	25μg, 100μg	Check More
APC Anti-Mouse CD69 Antibody[H1.2F3]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD69 Antibody[H1.2F3]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD69 Antibody[H1.2F3]	25μg, 100μg	Check More

Citations

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