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Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> FITC Anti-Mouse H-2 Antibody[M1/42]

FITC Anti-Mouse H-2 Antibody[M1/42]

  • Cat.No.:E-AB-F1216UC

  • Applications: FCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat
Applications:

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  • 25μg
  • 100μg
Price: $72
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Antigen Details

Alternate Names Mouse major histocompatibility complex (MHC) H-2, MHC I
References
1. Stallcup KC, et al. 1981. J. Immunol. 127:923.

Product Details

Form Liquid
Clone No. M1/42(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control FITC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833C]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation FITC
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KC et al, 1981). MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse H-2 Antibody[M1/42] 50μg, 500μg, 1mg Check More
Purified Anti-Mouse H-2 Antibody[M1/42] 25μg, 100μg Check More
Biotin Anti-Mouse H-2 Antibody[M1/42] 25μg, 100μg Check More
FITC Anti-Mouse H-2 Antibody[M1/42] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse H-2 Antibody[M1/42] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse H-2 Antibody[M1/42] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse H-2 Antibody[M1/42] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse H-2 Antibody[M1/42] 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse H-2 Antibody[M1/42] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Mouse H-2 Antibody[M1/42] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse H-2 Antibody[M1/42] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Red 780 Anti-Mouse H-2 Antibody[M1/42] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse H-2 Antibody[M1/42] 25μg, 100μg Check More
APC Anti-Mouse H-2 Antibody[M1/42] 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse H-2 Antibody[M1/42] 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse H-2 Antibody[M1/42] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse H-2 Antibody[M1/42] 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse H-2 Antibody[M1/42] 25μg, 100μg Check More
Elab Fluor® 488 Anti-Mouse H-2 Antibody[M1/42] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse H-2 Antibody[M1/42] 25μg, 100μg Check More
Elab Fluor® Red 780 Anti-Mouse H-2 Antibody[M1/42] 25μg, 100μg Check More

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