Alternate Names	Pgp-1, H-CAM, CD44s,CD44H
Swissprot	P26051
References	1. Arch R, et al. 1992. Science 257 (5070):682 2. Mitnacht R, et al. 1995. Eur. J. Immunol. 25(2):328 3. Zheng Z, et al. 1995. J Cell Bio. 130(2):485 4. Westermann J, et al. 1994. Eur. J. Immunol. 24(10):2312

Product Details

Form	Liquid
Clone No.	OX-49(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG2a, κ
Isotype Control	FITC Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802C]
Reactivity	Rat
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	FITC
	FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD44 is an 80-95 kD cell surface glycoprotein. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. It is up-regulated when T cells and B cells are activated. It was reported that CD44 is a valuable marker for memory T cells. CD44 is an adhesion molecule involved in leukocyte adhesion and homing to lymphoid organs. The OX-49 antibody reacts with CD44H (known as CD44s) expressed on most leukocytes, except for a subset of B lymphocytes. The epitope recognized by OX-49 antibody has been mapped to a region on both the standard, CD44s, and the splice variant, CD44v, isoforms of CD44. However it was reported that OX-49 antibody cannot detect the CD44V isoform, possibly due to conformational changes in the epitope.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
PE Anti-Rat CD44H Antibody[OX-49]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Rat CD44H Antibody[OX-49]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Rat CD44H Antibody[OX-49]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Rat CD44H Antibody[OX-49]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Rat CD44H Antibody[OX-49]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Rat CD44H Antibody[OX-49]	25μg, 100μg	Check More
PE Anti-Rat CD44H Antibody[OX-49]	25μg, 100μg	Check More
APC Anti-Rat CD44H Antibody[OX-49]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Rat CD44H Antibody[OX-49]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Rat CD44H Antibody[OX-49]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Rat CD44H Antibody[OX-49]	25μg, 100μg	Check More

Citations

Redox Biology (2023) IF: 10.787
Genistein mitigates senescence of bone marrow mesenchymal stem cells via ERRα-mediated mitochondrial biogenesis and mitophagy in ovariectomized rats

DOI: 10.1016/j.redox.2023.102649

Sample: bone marrow mesenchymal stem cells (BMMSCs)

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha