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Fluoride Resistant Acid Phosphatase (FRAP) Activity Assay Kit

Cat.No.:E-BC-K872-M
Detection instrument: Microplate reader (390-415 nm, optimum wavelength: 405 nm)

Detection method: Colorimetric method

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96T

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Detection principle

Fluoride resistant acid phosphatase (FRAP) is not inhibited by fluoride ions. It catalyzes the substrate to produce p-nitrophenol, which has a maximum absorption peak at 405 nm. The activity of FRAP can be calculated by measuring the OD value at 405 nm.

Performance characteristics

Synonyms	FRAP
Sample type	serum (plasma), cells and animal tissue samples
Sensitivity	0.61 U/L
Detection range	0.61 - 49.37 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	25 min
Precision	Average inter-assay CV: 8%Average intra-assay CV: 3.600%
Other instruments required	Incubator
Other reagents required	Double distilled water, Normal saline (0.9% NaCl)
Storage	-20℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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