English Toll-free:1-888-852-8623 or Contact Us
Keyword cannot be empty !

FOXO1 Polyclonal Antibody

Cat:E-AB-31466
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC-p;IF
Add to cart

For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:Hela
Dilution

WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthesized peptide derived from human FoxO1 around the non-phosphorylation site of Ser319.
Abbre FoxO1
Synonyms FKH 1;FKH1;FKHR;Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma);Forkhead box O1;Forkhead box protein O1;Forkhead box protein O1A;Forkhead in rhabdomyosarcoma;Forkhead;Drosophila;homolog of;in rhabdomyosarcoma;FoxO transcription factor;foxo1;FOXO1;FOXO1A;OTTHUMP00000018301
Swissprot
Calculated MW 70kDa
Observed MW 78kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer; Epigenetics and Nuclear Signaling; Metabolism; Neuroscience; Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.