GARS Polyclonal Antibody
Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
- Host: Rabbit
- Reactivity: Human; Mouse; Rat
- Applications: WB
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:Hela,A431,Mouse brain,HT29,Jurkat |
Dilution |
WB 1:500-1:2000 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Fusion protein of human GARS |
Abbre | GARS |
Synonyms | AP 4 A synthetase;Charcot Marie Tooth neuropathy 2D;Charcot Marie Tooth neuropathy neuronal type D;CMT2D ;Diadenosine tetraphosphate synthetase;DSMAV ;EC 6.1.1.14;Glycine tRNA ligase;Glycyl tRNA synthetase;GlyRS ;HMN5;SMAD1 |
Swissprot | |
Calculated MW | 83 kDa |
Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cytoplasm. Mitochondrion. |
Concentration | 0.6 mg/mL |
Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
Purification Method | Antigen affinity purification |
Research Areas | Cancer; Epigenetics and Nuclear Signaling |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene. |