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Glucose-6-phosphate dehydrogenase (G-6-PD) Activity Assay Kit

  • Cat.No.:E-BC-K056-M

  • Detection instrument: Microplate reader(450 nm)

To Purchase E-BC-K056-M

Size:
  • 96T
Price: $150
Qty:

Detection principle

Under the presence of G6PDH, glucose-6-phosphoric acid is oxidized to 6-PG. In this reaction, NADP+ is reduced to NADPH. Under the action of electron coupling reagent 1-MPMS, NADPH reducts wST-8 to form orange formazan, which has the maximum absorption peak at about 450 nm. Formazan generated in the reaction system is proportional to the activity of G6DPH in the sample.

Performance characteristics

Synonyms G-6-PD, G6PDH
Sample type Serum, plasma, animal tissue
Sensitivity 0.01 U/L
Detection range 0.01-50 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 20 min
Precision Average inter-assay CV: 5.8%Average intra-assay CV: 2.1%
Other instruments required Pipettor, Water bath, Centrifuge
Storage -20℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-50 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Rat serum

1

Rabbit serum

1

Cynomolgus monkey

1

10% Mouse liver tissue homogenate

3-5

10% Rat kidney tissue homogenate

1

10% Rat spleen tissue homogenate

5-10

10% Mouse brain tissue homogenate

1

Note: The diluent is reagent 1.

Citations

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Reviews/Q&A

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  • Reviews
  • Q&A
Q

P***************rSubmitted [ Jan 08 2022 ]

Asked: what\'s the minimum sample volume?

Reply

A

adminSubmitted [ Jan 08 2022 ]

Answered: The minimum sample volume is 50uL for each hole.

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