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Glucose (GLU) Fluorometric Assay Kit

  • Cat.No.:E-BC-F037

  • Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/590 nm)

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Price: $400

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Detection principle

Glucose oxidase can catalyze the oxidation of glucose into gluconic acid and produce hydrogen peroxide. In the presence of peroxidase, hydrogen peroxide reacts with the non-fluorescent substance to form fluorescent substance. The glucose content can be calculated indirectly by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Performance characteristics

Synonyms GLU
Sample type Serum, plasma, urine
Sensitivity 0.1 μmol/L
Detection range 0.1-20 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 25 min
Precision Average inter-assay CV: 2.8%Average intra-assay CV: 1.7%
Other instruments required Pipettor, Water bath, Centrifuge
Storage -20℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.1-20 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human plasma


Human serum


Chicken serum


Human urine


Human milk




Note: The diluent is reagent 1.


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