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Glucose (GLU) Fluorometric Assay Kit

Cat.No.:E-BC-F037
Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/590 nm)

Detection method: Fluorescence method

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Detection principle

Glucose oxidase can catalyze the oxidation of glucose into gluconic acid and produce hydrogen peroxide. In the presence of peroxidase, hydrogen peroxide reacts with the non-fluorescent substance to form fluorescent substance. The glucose content can be calculated indirectly by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Performance characteristics

Synonyms	GLU
Sample type	Serum, plasma, urine、saliva、milk、cell
Sensitivity	0.1 μmol/L
Detection range	0.1-20 μmol/L
Detection method	Fluorescence method
Assay type	Quantitative
Assay time	25 min
Precision	Average inter-assay CV: 2.800%Average intra-assay CV: 1.700%
Other instruments required	Pipettor, Water bath, Centrifuge
Storage	-20℃
Valid period	12 months

Images

Blood glucose level was significantly increased in STZ group (***P<0.001) and restored in treated group (**P<0.01).

J Liu et al investigate the function of circ-ITCH for improving renal inflammation and fibrosis in streptozotocin-induced mice. Glucose (GLU) concentration in mouse blood was determined using glucose fluorometric assay kit (E-BC-F037).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.1-20 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human plasma	300-600
Human serum	300-600
Chicken serum	600-1000
Human urine	1
Human milk	400-600
saliva	3-5

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

PHARMACOLOGICAL RESEARCH (2022) IF: 10.334
Sodium-Glucose Cotransporter-2 (SGLT2) expression in diabetic and non-diabetic failing human cardiomyocytes

DOI: 10.1016/j.phrs.2022.106448

PMID: 36096423
Sample: AC16 cell
MICROCHIMICA ACTA (2019) IF: 5.479
A gold electrode modified with a gold-graphene oxide nanocomposite for non-enzymatic sensing of glucose at near-neutral pH values

DOI: 10.1007/s00604-019-3796-8

Sample: sweat
INFLAMMATION RESEARCH (2021) IF: 4.575
CircRNA circ-ITCH improves renal inflammation and fibrosis in streptozotocin-induced diabetic mice by regulating the miR-33a-5p/SIRT6 axis

DOI: 10.1007/s00011-021-01485-8

PMID: 34216220
Sample: serum

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