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Glucose (GLU) Fluorometric Assay Kit

  • Cat.No.:E-BC-F037

  • Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/590 nm)

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  • 96T
  • 48T
Price: $400
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Detection principle

Glucose oxidase can catalyze the oxidation of glucose into gluconic acid and produce hydrogen peroxide. In the presence of peroxidase, hydrogen peroxide reacts with the non-fluorescent substance to form fluorescent substance. The glucose content can be calculated indirectly by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Performance characteristics

Synonyms GLU
Sample type Serum, plasma, urine
Sensitivity 0.1 μmol/L
Detection range 0.1-20 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 25 min
Precision Average inter-assay CV: 2.8%Average intra-assay CV: 1.7%
Other instruments required Pipettor, Water bath, Centrifuge
Storage -20℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.1-20 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human plasma

300-600

Human serum

300-600

Chicken serum

600-1000

Human urine

1

Human milk

400-600

saliva

3-5

Note: The diluent is reagent 1.

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

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