Glucose Uptake Fluorometric Assay Kit (E-BC-F041)
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For research use only.
| Detection Principle | 2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 530 nm and the emission wavelength of 590 nm. |
| Detection Method | Fluorometric method |
| Sample Type | Cell |
| Assay Time | 1 h 40 min |
| Detection Instrument | Fluorescence microplate reader |
| Ex/Em | Fluorescence microplate reader (Ex/Em=530 nm/590 nm) |
| Other Reagents Required | KRPH solution (20 mM Hepes, 5 mM KH2PO4, 1 mM MgSO4, 1 mM CaCl2, 136 mM NaCl, 4.7 mM KCl, 2% BSA, pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Expiration date | 12 months |
| Shipping | Ice bag |
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