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GLUT-1 Polyclonal Antibody

Cat:E-AB-31556 Citations (3)
Manual MSDS
  • +1

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC-p;IF
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Product Details
Verified Samples Verified Samples in WB:K562
Verified Samples in IHC:Rat kidney
Verified Samples in IF:Rat lung
Dilution

WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthesized peptide derived from the C-terminal region of human Glut1
Abbre Glut1
Synonyms Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity);CSE;DYT17;DYT18;DYT9;EIG12;erythrocyte/brain;Erythrocyte/hepatoma glucose transporter;facilitated glucose transporter member 1;Glucose transporter 1;Glucose transporter type 1;Glucose transporter type 1;erythrocyte/brain;GLUT;GLUT-1;GLUT1;GLUT1DS;GLUTB;GT1;GTG1;Gtg3;GTR1;HepG2 glucose transporter;HTLVR;Human T cell leukemia virus (I and II) receptor;MGC141895;MGC141896;PED;RATGTG1;Receptor for HTLV 1 and HTLV 2;SLC2A1;Solute carrier family 2 (facilitated glucose transporter);member 1;Solute carrier family 2;Solute carrier family 2;facilitated glucose transporter member 1
Swissprot
Calculated MW 54kDa
Observed MW 55kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Membrane
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer; Cardiovascular; Metabolism; Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background This gene encodes a major glucose transporter in the mammalian blood-brain barrier. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.