GLUT-1 Polyclonal Antibody
- +1
Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB;IHC-p;IF
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:K562 Verified Samples in IHC:Rat kidney Verified Samples in IF:Rat lung |
Dilution |
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Synthesized peptide derived from the C-terminal region of human Glut1 |
Abbre | Glut1 |
Synonyms | Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity);CSE;DYT17;DYT18;DYT9;EIG12;erythrocyte/brain;Erythrocyte/hepatoma glucose transporter;facilitated glucose transporter member 1;Glucose transporter 1;Glucose transporter type 1;Glucose transporter type 1;erythrocyte/brain;GLUT;GLUT-1;GLUT1;GLUT1DS;GLUTB;GT1;GTG1;Gtg3;GTR1;HepG2 glucose transporter;HTLVR;Human T cell leukemia virus (I and II) receptor;MGC141895;MGC141896;PED;RATGTG1;Receptor for HTLV 1 and HTLV 2;SLC2A1;Solute carrier family 2 (facilitated glucose transporter);member 1;Solute carrier family 2;Solute carrier family 2;facilitated glucose transporter member 1 |
Swissprot | |
Calculated MW | 54kDa |
Observed MW |
55kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Membrane |
Concentration | 1 mg/mL |
Buffer | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4 |
Purification Method | Affinity purification |
Research Areas | Cancer; Cardiovascular; Metabolism; Signal Transduction |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | This gene encodes a major glucose transporter in the mammalian blood-brain barrier. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses. |
-
Oligo-Fucoidan supplementation enhances the effect of Olaparib on preventing metastasis and recurrence of triple-negative breast cancer in mice
IF:12.771
Journal:JOURNAL OF BIOMEDICAL SCIENCE
DOI:10.1186/s12929-022-00855-6
PMID:36109724
-
The pivotal role of miRNA-21 in myocardial metabolic flexibility in response to short- and long-term high glucose treatment: Evidence in human cardiomyocyte cell line
IF:8.18
Journal:DIABETES RESEARCH AND CLINICAL PRACTICE
DOI:10.1016/j.diabres.2022.110066
PMID:36058441
-
Glutor, a Glucose Transporter Inhibitor, Exerts Antineoplastic Action on Tumor Cells of Thymic Origin: Implication of Modulated Metabolism, Survival, Oxidative Stress, Mitochondrial Membrane Potential, pH Homeostasis, and Chemosensitivity
IF:5.738
Journal:Frontiers in Oncology