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Glutamic Acid Colorimetric Assay Kit

Cat.No.:E-BC-K118-S
Detection instrument: Spectrophotometer(340 nm)

Detection method: Colorimetric method

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Detection principle

Glutamic acid can react with NAD+ under the catalysis of glutamate dehydrogenase to produce α-ketoglutaric acid, NADH and NH4+. NADH has the maximum absorption at 340 nm. And glutamic acid content can be calculated by measuring the change of NADH.

Performance characteristics

Sample type	Serum,plasma,animal tissue,cells,cell culture supernatant
Sensitivity	4.00 μmol/L
Detection range	4.00-450 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 2.900%Average intra-assay CV: 2.200%
Other instruments required	Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	Reagent 1, Reagent 2, Reagent 8, Reagent 9: 2-8℃; Reagent 3, Reagent 4, Reagent 5, Reagent 6, Reagent 7: -20℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (4.00-450 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1
10% Rat liver tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat brain tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

EUROPEAN JOURNAL OF PHARMACOLOGY (2022) IF: 5.195
Identification of a new small molecule that initiates ferroptosis in cancer cells by inhibiting the system Xc− to deplete GSH

DOI: 10.1016/j.ejphar.2022.175304

Sample: U2OS cell

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