Proline (Pro) Colorimetric Assay Kit (E-BC-K177-S)
For research use only.
| Detection Principle | Proline can react with acidic-ninhydrin to form stable red compound. The maximum absorption peak of the compound is at 520 nm. And the concentration of Pro can be calculated by measuring the OD value at 520 nm. |
| Synonyms | Pro |
| Sample Type | Plant tissue,Honey |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (520 nm) |
| Research Area | Amino Acids And Proteins , Plant Physiology Series |
| Other Reagents Required | Acetic acid, Methylbenzene, Absolute ethyl alcohol |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.17 μg/mL |
| Detection Range | 0.17-35 μg/mL |
| Precision | inter-assay CV: 5.4% | intra-assay CV: 4.5% |
| Sample Volume | 2 mL(honey/tissue homogenate) |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Green pepper tissue homogenization | 1 |
| Carrot tissue homogenization | 1 |
| Epipremnum aureum tissue homogenization | 1 |
| Lettuce leaf tissue homogenization | 1 |
| Honey | 1 |
| Cabbages tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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