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Glutathione Peroxidase (GSH-Px) Activity Assay Kit

  • Cat.No.:E-BC-K096-S

  • Detection instrument: Spectrophotometer(412 nm)

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Detection principle

Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.

Performance characteristics

Synonyms GSH-PX
Sample type Serum,plasma,cells,cell culture supernatant,tissue
Sensitivity 12.65 U
Detection range 12.65-387 U
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 70 min
Precision Average inter-assay CV: 9.300%Average intra-assay CV: 4.900%
Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

A significant decline of GSH-Px activity due to ovariectomy (OVX) was observed (P=0.039). Treatment with annatto tocotrienol (ANTT) and Raloxifene (RAL) elevated GSH-Px activity.

N V Mohamad et al investigate the therapeutic effect of annatto tocotrienol for postmenopausal bone loss. Glutathione peroxidase (GSH-Px) activity of rat serum was determined using GSH-Px activity assay kit (E-BC-K096-S).

Dilution of sample

The optimal sampling volume are different for different species, also are different for different sample type. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the inhibition ratio is 20%~60% (the optimal inhibition ratio is the range of 45%~55%.) before formal experiment.

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. Antioxidants (2022) IF: 7.675
    Inhibition of Tumor Growth and Modulation of Antioxidant Activity of Rhodoxanthin Isolated from Taxus baccata Aril against B16F10 Murine Malignant Melanoma

    DOI: 10.3390/antiox11112264

    Sample: plasma
  2. FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 6.025
    Procyanidins inhibit zearalenone-induced apoptosis and oxidative stress of porcine testis cells through activation of Nrf2 signaling pathway

    DOI: 10.1016/j.fct.2022.113061

    PMID: 35489465

    Sample: pig testicular cell (ST cell)
  3. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    D-ribose-L-cysteine prevents oxidative stress and cardiometabolic syndrome in high fructose high fat diet fed rats

    DOI: 10.1016/j.biopha.2021.112017

    PMID: 34399203

    Sample: heart
  4. Nutrients (2022) IF: 6.706
    Cornuside Is a Potential Agent against Alzheimer’s Disease via Orchestration of Reactive Astrocytes

    DOI: 10.3390/nu14153179

    Sample: hippocampus,cortex
  5. Antioxidants (2020) IF: 5.014
    Dietary Postbiotic Lactobacillus plantarum Improves Serum and Ruminal Antioxidant Activity and Upregulates Hepatic Antioxidant Enzymes and Ruminal Barrier Function in Post-Weaning Lambs

    DOI: 10.3390/antiox9030250

    Sample: serum,ruminal fluid
  6. LIFE SCIENCES (2021) IF: 5.037
    Protective effects of low-temperature plasma on cisplatin-induced nephrotoxicity

    DOI: 10.1016/j.lfs.2021.120230

    PMID: 34919900

  7. Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
    Ameliorative Effects of Bredemolic Acid on Markers Associated with Renal Dysfunction in a Diet-Induced Prediabetic Rat Model

    DOI: 10.1155/2020/2978340

    PMID: 32655765

    Sample: Plasma,Urine,Tissue homogenate
  8. Marine Drugs (2022) IF: 5.118
    Anti-Photoaging Effect of Hydrolysates from Pacific Whiting Skin via MAPK/AP-1, NF-κB, TGF-β/Smad, and Nrf-2/HO-1 Signaling Pathway in UVB-Induced Human Dermal Fibroblasts

    DOI: 10.3390/md20050308

    Sample: Human dermal fibroblasts (HDFs)
  9. CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
    In vitro and in vivo anticancer effects of syringic acid on colorectal cancer: Possible mechanistic view

    DOI: 10.1016/j.cbi.2020.109337

    PMID: 33548266

    Sample: Cell sample
  10. Journal of Environmental Sciences (2022) IF: 5.565
    Alleviative effects of a novel strain Bacillus coagulans XY2 on copper-induced toxicity in zebrafish larvae

    DOI: 10.1016/j.jes.2022.02.010

    PMID: 36375957

    Sample: larvae
  11. Journal of Clinical Medicine (2018) IF: 5.583
    Supplementation of Short-Chain Fatty Acid, Sodium Propionate, in Patients on Maintenance Hemodialysis: Beneficial Effects on Inflammatory Parameters and Gut-Derived Uremic Toxins, A Pilot Study (PLAN Study)

    DOI: 10.3390/jcm7100315

    PMID: 30274359

    Sample: Serum
  12. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Comparison of Selenium Nanoparticles and Sodium Selenite on the Alleviation of Early Atherosclerosis by Inhibiting Endothelial Dysfunction and Inflammation in Apolipoprotein E-Deficient Mice

    DOI: 10.3390/ijms222111612

    PMID: 34769040

    Sample: serum
  13. Frontiers in Pharmacology (2022) IF: 5.988
    ELA-11 protects the heart against oxidative stress injury induced apoptosis through ERK/MAPK and PI3K/AKT signaling pathways

    DOI: 10.3389/fphar.2022.873614

    PMID: 36160397

    Sample: primary cardiomyocyte
  14. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
    Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

    DOI: 10.1016/j.sjbs.2022.103495

    Sample: testicular tissue
  15. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    Differential neuroprotective effect of curcuminoid formulations in aluminum chloride–induced Alzheimer’s disease

    DOI: 10.1007/s11356-022-20593-4

    Sample: brain tissue
  16. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

    DOI: 10.1016/j.jep.2022.115306

    PMID: 35443217

    Sample: serum
  17. MOLECULAR IMMUNOLOGY (2021) IF: 4.407
    LncRNA NKILA relieves astrocyte inflammation and neuronal oxidative stress after cerebral ischemia/reperfusion by inhibiting the NF-κB pathway

    DOI: 10.1016/j.molimm.2021.08.002

    PMID: 34454183

    Sample: brain tissue,HT-22 cell
  18. MOLECULES (2021) IF: 4.412
    Insight into Seasonal Change of Phytochemicals, Antioxidant, and Anti-Aging Activities of Root Bark of Paeonia suffruticosa (Cortex Moutan) Combined with Multivariate Statistical Analysis

    DOI: 10.3390/molecules26206102

    PMID: 34684685

    Sample: HDF cell
  19. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2019) IF: 4.527
    Chronic neurodegeneration by aflatoxin B1 depends on alterations of brain enzyme activity and immunoexpression of astrocyte in male rats

    DOI: 10.1016/j.ecoenv.2019.109407

    PMID: 31279280

    Sample: Tissue homogenate
  20. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino rats

    DOI: 10.1016/j.biopha.2020.109841

    PMID: 31972360

    Sample: Tissue homogenate
  21. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Semen Brassicae reduces thoracic aortic remodeling, inflammation, and oxidative damage in spontaneously hypertensive rats

    DOI: 10.1016/j.biopha.2020.110400

    PMID: 32570115

    Sample: Cell sample
  22. SAUDI PHARMACEUTICAL JOURNAL (2022) IF: 4.562
    Mechanism of action of Orthosiphon stamineus against non-alcoholic fatty liver disease: Insights from systems pharmacology and molecular docking approaches

    DOI: 10.1016/j.jsps.2022.09.001

    Sample: HepG2 cell
  23. PROCESS BIOCHEMISTRY (2022) IF: 4.885
    Vernodalin regulated the NF-κβp65 signaling in inflammation of lipopolysaccharide -induced sepsis rats

    DOI: 10.1016/j.procbio.2022.10.004

    Sample: liver,kidney,lungs
  24. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2022) IF: 3.094
    Native SodB Overexpression of Synechocystis sp. PCC 6803 Improves Cell Growth Under Alcohol Stresses Whereas Its Gpx2 Overexpression Impacts on Growth Recovery from Alcohol Stressors

    DOI: 10.1007/s12010-022-04061-w

    PMID: 35819692

    Sample: PCC 6803 strains
  25. EUROPEAN JOURNAL OF PHARMACOLOGY (2019) IF: 3.17
    Intraocular pressure-lowering effects of imidazo[1,2-a]- and pyrimido[1,2-a]benzimidazole compounds in rats with dexamethasone-induced ocular hypertension

    DOI: 10.1016/j.ejphar.2019.01.059

    Sample: Tissue homogenate
  26. Animals (2022) IF: 3.231
    Milk Pathogens in Correlation with Inflammatory, Oxidative and Nitrosative Stress Markers in Goat Subclinical Mastitis

    DOI: 10.3390/ani12233245

    Sample: skimmed milk
  27. Environmental Research Communications (2022) IF: 3.237
    Metal accumulation in muscle and oxidative stress response in the liver of juvenile Oreochromis niloticus from contaminated sediment under a simulation of increasing temperature

    DOI: 10.1088/2515-7620/ac803d

    Sample: liver
  28. Bioengineered (2022) IF: 3.269
    Methylprednisolone up-regulates annexin A1 (ANXA1) to inhibit the inflammation, apoptosis and oxidative stress of cigarette smoke extract (CSE)-induced bronchial epithelial cells, a chronic obstructive pulmonary disease in vitro model, through the formyl

    DOI: 10.1080/21655979.2022.2031769

    Sample: BEAS-2B cells
  29. NEUROTOXICITY RESEARCH (2019) IF: 3.311
    Antiproliferative Effect of Elastin-Derived Peptide VGVAPG on SH-SY5Y Neuroblastoma Cells

    DOI: 10.1007/s12640-019-00040-y

    PMID: 31161598

    Sample: SH-SY5Y cells
  30. Hormones-International Journal of Endocrinology and Metabolism (2022) IF: 3.419
    High chitotriosidase and AGE levels in acromegaly: a case-control study

    DOI: 10.1007/s42000-022-00409-3

    PMID: 36241955

    Sample: serum
  31. Frontiers in Behavioral Neuroscience (2022) IF: 3.558
    Acupuncture Relieves Stress-Induced Depressive Behavior by Reducing Oxidative Stress and Neuroapoptosis in Rats

    DOI: 10.3389/fnbeh.2021.783056

    PMID: 35058758

    Sample: hippocampus tissue
  32. JOURNAL OF APPLIED TOXICOLOGY (2022) IF: 3.628
    Asiaticoside attenuates oxygen-glucose deprivation/reoxygenation-caused injury of cardiomyocytes by inhibiting autophagy

    DOI: 10.1002/jat.4424

    Sample: AC16 cells
  33. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022) IF: 3.639
    Flaxseed oil ameliorates aging in D-galactose induced rats via altering gut microbiota and mitigating oxidative damage

    DOI: 10.1002/jsfa.12010

    PMID: 35567370

    Sample: serum
  34. PLANT PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 3.72
    Overexpression of ethylene response factor ERF96 gene enhances selenium tolerance in Arabidopsis

    DOI: 10.1016/j.plaphy.2020.02.024

    PMID: 32097848

    Sample: Plant Tissue
  35. Annals of Translational Medicine (2022) IF: 3.932
    Levosimendan protects from sepsis-inducing cardiac dysfunction by suppressing inflammation, oxidative stress and regulating cardiac mitophagy via the PINK-1-Parkin pathway in mice

    DOI: 10.21037/atm-22-483

    PMID: 35280364

    Sample: myocardium
  36. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
    Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic rats

    DOI: 10.1016/j.intimp.2020.106582

    PMID: 32438076

    Sample: Tissue homogenate
  37. Scientific Reports (2020) IF: 3.998
    Quercetin liposomes ameliorate streptozotocin-induced diabetic nephropathy in diabetic rats

    DOI: 10.1038/s41598-020-59411-7

    Sample: kidney tissue
  38. MOLECULAR VISION (2017) IF: 2.057
    Pinus densiflora bark extract prevents selenite-induced cataract formation in the lens of Sprague Dawley rat pups

    PMID: 28943754

    Sample: lens tissue
  39. Biomolecules & Therapeutics (2017) IF: 2.075
    Hepaprotective Effect of Standardized Ecklonia stolonifera Formulation on CCl4-Induced Liver Injury in Sprague-Dawley Rats

    DOI: 10.4062/biomolther.2017.199

    PMID: 29129047

    Sample: Tissue homogenate
  40. International Journal of General Medicine (2023) IF: 2.145
    Hematological, Inflammatory, Coagulation, and Oxidative/Antioxidant Biomarkers as Predictors for Severity and Mortality in COVID-19: A Prospective Cohort-Study

    DOI: 10.2147/IJGM.S402206

    Sample: Serum
  41. MOLECULAR VISION (2019) IF: 2.174
    Polyphenol-enriched fraction of Vaccinium uliginosum L. protects selenite-induced cataract formation in the lens of Sprague-Dawley rat pups

    PMID: 30820147

    Sample: lens tissue
  42. CHEMISTRY & BIODIVERSITY (2021) IF: 2.408
    Traditional Chinese Medicine Ganshuang Granules Attenuate CCl4-Induced Hepatic Fibrosis by Modulating Gut Microbiota

    DOI: 10.1002/cbdv.202100520

    Sample: liver
  43. Cardiovascular Therapeutics (2020) IF: 2.538
    Bredemolic Acid Improves Cardiovascular Function and Attenuates Endothelial Dysfunction in Diet-Induced Prediabetes: Effects on Selected Markers

    DOI: 10.1155/2020/1936406

    PMID: 32117470

    Sample: Plasma,Tissue homogenate
  44. JOURNAL OF PHARMACY AND PHARMACOLOGY (2020) IF: 2.571
    By reducing oxidative stress, naringenin mitigates hyperglycaemia‐induced upregulation of hepatic nuclear factor erythroid 2‐related factor 2 protein

    DOI: 10.1111/jphp.13319

    Sample: Cell lysate
  45. Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
    Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

    DOI: 10.1002/jez.2603

    Sample: cortex
  46. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 2.72
    Effect of coconut water and milk on heat stress-induced gastrointestinal tract dysmotility in rats: Role of oxidative stress and inflammatory response

    DOI: 10.1111/jfbc.14129

    PMID: 35298033

    Sample: gastric,jejunal, colonic
  47. JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
    Antioxidant and antithrombotic effects of green mussels (Perna canaliculus) in rats

    DOI: 10.1111/jfbc.13865

    PMID: 34263474

    Sample: Liver,kidney
  48. BMC Complementary and Alternative Medicine (2020) IF: 2.833
    HPLC-DAD analysis of Hyssopus Cuspidatus Boriss extract and mensuration of its antioxygenation property

    DOI: 10.1186/s12906-020-03016-0

    PMID: 32689984

    Sample: Plant Fluid
  49. Journal of Diabetes Research (2018) IF: 2.885
    The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers

    DOI: 10.1155/2018/7341242

    Sample: kidney tissues
  50. CURRENT ISSUES IN MOLECULAR BIOLOGY (2023) IF: 2.976
    Chemopreventive Effects of Onosma mutabilis against Azoxymethane-Induced Colon Cancer in Rats via Amendment of Bax/Bcl-2 and NF-κB Signaling Pathways

    DOI: 10.3390/cimb45020057

    Sample: colonic tissue
  51. ANNALS OF VASCULAR SURGERY (2020) IF: 1.125
    Which Distant Organ is Most Affected by Lower Extremity Ischemia-Reperfusion?

    DOI: 10.1016/j.avsg.2020.01.008

    PMID: 31927058

    Sample: Tissue homogenate
  52. Experimental and Therapeutic Medicine (2018) IF: 1.41
    Rutin inhibits coronary heart disease through ERK1/2 and Akt signaling in a porcine model

    DOI: 10.3892/etm.2017.5365

    PMID: 29250160

    Sample: Urine
  53. JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662
    The effects of chrysin on lipopolysaccharide‐induced sepsis in rats

    DOI: 10.1111/jfbc.13359

    Sample: lung,liver,kidney
  54. CURRENT EYE RESEARCH (2020) IF: 1.754
    Expression of α-Klotho Is Downregulated and Associated with Oxidative Stress in the Lens in Streptozotocin-induced Diabetic Rats

    DOI: 10.1080/02713683.2020.1805768

    PMID: 32744464

    Sample: Tissue homogenate
  55. Molecular Medicine Reports (2018) IF: 1.922
    Isopsoralen regulates PPAR‑γ/WNT to inhibit oxidative stress in osteoporosis

    DOI: 10.3892/mmr.2017.7954

    Sample: Serum
  56. Journal of Evidence-Based Integrative Medicine (2019)
    Methanol-Extract/Fractions of Dacryodes edulis Leaves Ameliorate Hyperglycemia and Associated Oxidative Stress in Streptozotocin-Induced Diabetic Wistar Rats:

    DOI: 10.1177/2515690X19843832

  57. Journal of Nutrition and Metabolism (2020)
    Digestive Fermentation, Antioxidant Status, and Haemato-Biochemical Indices of Growing Rabbits Fed on Diets Supplemented with Ziziphus spina-christi Leaf

    DOI: 10.1155/2020/9046862

    PMID: 32318290

    Sample: Serum
  58. Toxicology Reports (2017)
    Diclofenac – induced hepatotoxicity: Low dose of omega-3 fatty acids have more protective effects

    DOI: 10.1016/j.toxrep.2017.12.002

    Sample: plasma
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

L*mSubmitted [ Dec 17 2023 ]

Asked: From the manual Appendix II Sample Preparation part, for the cells sample, it is stated that (3) Treat the cells with ultrasonic cell disruptor (200 W, 2 s/time, interval for 3 s, the total time is 10 min). However, the ultrasonicator in our laboratory is QSONICA Q500 Sonicator which operating at 500 Watts, 20 kHz and 30% amplitude/intensity. May I ask if using this ultrasonicator parameter, what is the sonication duration (second/time), interval duration and total time you would recommended and required in order to lyse the HepG2 cells (around 1 x 10^6 / 500 µL) for GSH-Px detection in this assay?

Reply

A

adminSubmitted [ Dec 17 2023 ]

Answered: The ultrasonic cell disruptor parameters of broken cells given in our instructions are for reference only, and can be adjusted appropriately for different instruments. You can perform ultrasonicator with parameters commonly used in the laboratory, as long as the purpose of cell breakage is achieved.

Q

A***********nSubmitted [ May 25 2023 ]

Asked: Hi, may I know that this kit is suitable using rat\'s liver for hepatoprotective test?

Reply

A

adminSubmitted [ May 25 2023 ]

Answered: Hi Amil Benjamin, E-BC-K096-M/S are suitalbe using rat's liver for hepatoprotective test.

Q

S****************iSubmitted [ Mar 29 2023 ]

Asked: Can we use this kit for saliva

Reply

A

adminSubmitted [ Mar 29 2023 ]

Answered: Hello Shalini Pasupuleti,thank you for your support.This kit you inquire is suitable for saliva samples.

Q

M*****aSubmitted [ Oct 14 2022 ]

Asked: We only look at the size where its mentioned 100 assays. So, could the kit be used for 100 samples?

Reply

A

adminSubmitted [ Oct 14 2022 ]

Answered: 100 Assays represents 100 times and 48 samples can be tested. 100Assays= 2 blank tubes +2 standard tubes + 48 non-enzyme tubes +48 enzyme tubes (each sample need a control)

Q

M*****aSubmitted [ Sep 14 2022 ]

Asked: Can the kits detect cells that have been lysed in RIPA buffer

Reply

A

adminSubmitted [ Sep 14 2022 ]

Answered: Lysates are not recommended for enzymatic indexes, which may affect the determin. This kit has its own homogenate medium.

Q

A***********fSubmitted [ Aug 30 2022 ]

Asked: Kindly respond, can we use 405nm spectrophotometry filter? Or alternatively can this be done in a microplate instead? Regards

Reply

A

adminSubmitted [ Aug 30 2022 ]

Answered: The optimal detection wavelength is 412nm for this kit. There may be a little error if using 405nm spectrophotometry filter. The whole trend of samples will be ok. 405nm spectrophotometry filter could be a choice if you don't have 412nm spectrophotometry filter. It couldn't be used in a microplate reader.

Q

F**oSubmitted [ Dec 19 2020 ]

Asked: I confused about the blank tube. If I have double beam spektro, what solution do i use for zeroing? And what blank for the standard & sample ?

Reply

A

adminSubmitted [ Dec 19 2020 ]

Answered: Hi Faro, Thanks for your message. Could you let us know which country you are from, so that I could forward to our local sales representative to contact you ASAP. Best regards

Q

T****************gSubmitted [ Apr 23 2019 ]

Asked: We don\'t have 412 nm of colorimetric spectro filter in my lab, we only have the 415 nm. And so, is it ok if we use 415nm? would it effect the accuracy of the measurement significantly? Could give me the contract of distributor in Thailand. Thank you.

Reply

A

adminSubmitted [ Apr 23 2019 ]

Answered: Dear Thapanee, Thanks for your message. It is ok to use 412nm to test the kit. Pls contact with the distributor to have the kit. Pacific Science Co.,Ltd. 62,64 Soi Charansanitwong 49/1 Charansanitwong rd. Bangbumru Bangplad Bangkok 10700 THAILAND Tel : +662-4330068-9 ext. 212 Email : pacscien@ksc.th.com Any questions, pls feel free to ask. Best regards,

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