Glutathione Peroxidase (GSH-Px) Activity Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K096-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $260
      Can detect 48 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(412 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.

      Performance characteristics

      Synonyms GSH-PX
      Sample type serum,plasma,cells,cell culture supernatant,tissue
      Sensitivity 12.65 U
      Detection range 12.65-387 U
      Precision Average inter-assay CV: 9.3%Average intra-assay CV: 4.9%
      Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months

      Dilution of sample

      The optimal sampling volume are different for different species, also are different for different sample type. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the inhibition ratio is 20%~60% (the optimal inhibition ratio is the range of 45%~55%.) before formal experiment.

      Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

      Citations

      1. Pinus Densiflora Bark Extract Prevents Selenite-induced Cataract Formation In The Lens Of Sprague Dawley Rat Pups.
        IF: 2.057
        Journal: Molecular Vision(2017)
        PMID: 28943754
        Products: E-EL-0026 E-EL-0060 E-BC-K096-S 
        Sample type: Tissue homogenate
        Reactivity: Rat
      2. The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers.
        IF: 2.885
        Journal: Journal of Diabetes Research(2018)
        DOI: 10.1155/2018/7341242
        PMID: 30009183
        Products: E-BC-K096-S E-BC-K020-M E-EL-0070 
        Reactivity: Rat
      3. Intraocular Pressure-Lowering Effects of Imidazo [1, 2-α]-and Pyrimido [1, 2-α] Benzimidazole Compounds in Rats with Dexamethasone-Induced Ocular Hypertension
        IF: 3.17
        Journal: European Journal of Pharmacology(2019)
        DOI: 10.1016/j.ejphar.2019.01.059
        PMID: 30716317
        Products: E-BC-K020-M E-BC-K096-S E-BC-K031-S 
        Sample type: Tissue homogenate
        Reactivity: Rat
      4. Supplementation of Short-Chain Fatty Acid, Sodium Propionate, in Patients on Maintenance Hemodialysis: Beneficial Effects on Inflammatory Parameters and Gut-Derived Uremic Toxins, A Pilot Study (PLAN Study)
        IF: 5.688
        Journal: Journal of Clinical Medicine(2018)
        DOI: 10.3390/jcm7100315
        PMID: 30274359
        Products: E-BC-K096-S 
        Sample type: Serum
        Reactivity: Human
      5. The Effects of Momordica Balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers
        IF: 3.04
        Journal: Journal of Diabetes Research(2018)
        DOI: 10.1155/2018/7341242
        PMID: 30009183
        Products: E-BC-K020-M E-BC-K096-S 
        Sample type: Tissue homogenate
        Reactivity: Rat
      6. Pinus Densiflora Bark Extract Prevents Selenite-induced Cataract Formation In The Lens Of Sprague Dawley Rat Pups
        IF: 2.174
        Journal: Molecular Vision(2017)
        PMID: 28943754
        Products: E-BC-K096-S 
        Sample type: Cataractous lenses
        Reactivity: Rat
      7. Effect of Memantine Hydrochloride on Cisplatin-induced Toxicity with Special Reference to Renal Alterations in Mice
        IF: 0.765
        Journal: International Journal of Pharmacology(2019)
        DOI: 10.3923/ijp.2019.189.199
        Products: E-BC-K025-S 
        Sample type: Tissue homogenate
        Reactivity: Mouse
      8. Hepaprotective Effect of Standardized Ecklonia stolonifera Formulation on CCl4-Induced Liver Injury in Sprague-Dawley Rats
        IF: 3.089
        Journal: Biomolecules & Therapeutics(2018)
        DOI: 10.4062/biomolther.2017.199
        PMID: 29129047
        Products: E-BC-K030-M 
        Sample type: Tissue homogenate
        Reactivity: Rat
      9. Rutin inhibits coronary heart disease through ERK1/2 and Akt signaling in a porcine model
        IF: 1.448
        Journal: Experimental and Therapeutic Medicine(2018)
        DOI: 10.3892/etm.2017.5365
        PMID: 29250160
        Products: E-BC-K019-S 
        Sample type: Urine
        Reactivity: Porcine
      10. Antiproliferative Effect of Elastin-Derived Peptide VGVAPG on SH-SY5Y Neuroblastoma Cells
        IF: 3.311
        Journal: Neurotoxicity Research(2019)
        DOI: 10.1007/s12640-019-00040-y
        PMID: 31161598
        Products: E-BC-K031-S 
        Sample type: SH-SY5Y cells
        Reactivity: Human
      11. Isopsoralen regulates PPAR‑γ/WNT to inhibit oxidative stress in osteoporosis
        IF: 1.851
        Journal: Molecular Medicine Reports(2018)
        DOI: 10.3892/mmr.2017.7954
        PMID: 29115612
        Products: E-BC-K091-S 
        Sample type: Serum
        Reactivity: Rat
      12. Polyphenol-enriched fraction of Vaccinium uliginosum L. protects selenite-induced cataract formation in the lens of Sprague-Dawley rat pups
        IF: 2.174
        Journal: Molecular Vision(2019)
        PMID: 30820147
        Products: E-BC-K030-M 
        Sample type: Lens
        Reactivity: Rat
      13. Chronic neurodegeneration by aflatoxin B1 depends on alterations of brain
        IF: 4.527
        Journal: Ecotoxicology and Environmental Safety(2019)
        DOI: 10.1016/j.ecoenv.2019.109407
        Products: E-BC-K117-S E-BC-K025-S E-BC-K030-M E-BC-K031-S E-BC-K096-S 
        Sample type: Tissue homogenate
        Reactivity: Rat
      14. Modulation the Neuro-toxicity Induced by Aluminum Chloride in Rats Using Beetroots and Broccli
        Journal: Journal of Pharmaceutical Research International(2018)
        DOI: 10.9734/jpri/2018/v25i330100
        Products: E-BC-K136-S E-BC-K096-S 
        Sample type: Tissue homogenate
        Reactivity: Rat
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      Q

      T****************gSubmitted [ Apr 23 2019 ]

      Asked: We don\'t have 412 nm of colorimetric spectro filter in my lab, we only have the 415 nm. And so, is it ok if we use 415nm? would it effect the accuracy of the measurement significantly? Could give me the contract of distributor in Thailand. Thank you.

      Reply

      A

      adminSubmitted [ Apr 23 2019 ]

      Answered: Dear Thapanee, Thanks for your message. It is ok to use 412nm to test the kit. Pls contact with the distributor to have the kit. Pacific Science Co.,Ltd. 62,64 Soi Charansanitwong 49/1 Charansanitwong rd. Bangbumru Bangplad Bangkok 10700 THAILAND Tel : +662-4330068-9 ext. 212 Email : pacscien@ksc.th.com Any questions, pls feel free to ask. Best regards,

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