Glutathione Peroxidase (GSH-Px) Activity Assay Kit

    • Biochemical Assay Kit-Elabscience
    <
    >
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K096-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $260

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer

      Valid period: 6 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      General information

      Detection significance

      Glutathione Peroxidase (GSH-PX) is an important enzyme that catalyzes decomposition of hydrogen peroxide. GSH specifically catalyze the reaction between GSH and hydrogen peroxide, protecting cell membrane structure and keeping membrane function integrity. Se-cysteine is the active center of the GSH-PX. Determination of GSH-PX activity in organism can be an indicator of selenium level as Se is essential section of GSH-PX.

      Detection principle

      Glutathione Peroxidase (GSH-PX) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-PX, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.

      The key point

      If the GSH-PX activity is calculated by protein concentration, the protein concentration of the sample needs to be determined separately (E-BC-K318-M, E-BC-K168-S, E-BC-K165-S).

      Operation procedures

      Operation table

      1.       For tissue, cells and cell culture supernatant sample:

      1)        Enzymatic reaction (Reagent 1 application solution are pre-warmed in advance at 37)

       

      Non-enzyme tube

      Enzyme tube

      1 mmol/L GSH (mL)

      0.2

      0.2

      Sample (mL)

       

      0.2

      Pre-heat the tubes at 37 water bath for 5 min. Preheat Reagent 1 application solution at 37 for 5 min at the same time.

      Reagent 1 application solution (mL)

      0.1

      0.1

      React at 37 water bath for 5 min accurately.

      Reagent 2 (mL)

      2

      2

      Sample (mL)

      0.2

       

      Mix fully and centrifuge at 3100 g for 10 min, then take 1 mL of supernatant for chromogenic reaction.


       

      2)        Chromogenic reaction


       

      Blank tube

      Standard tube

      Non-enzyme tube

      Enzyme tube

      GSH standard application solution (mL)

      1

       

       

       

      20 μmol/L GSH standard solution (mL)

       

      1

       

       

      Supernatant (mL)

       

       

      1

      1

      Reagent 3 application solution (mL)

      1

      1

      1

      1

      Reagent 4 (mL)

      0.25

      0.25

      0.25

      0.25

      Reagent 5 application solution (mL)

      0.05

      0.05

      0.05

      0.05

           Mix fully and stand for 15 min at room temperature. Set to zero with double-distilled water and measure the OD values of each tube at 412 nm with 1 cm optical path quartz cuvette.

       

      2.       For serum/plasma sample:

      1)        Enzymatic reaction (Reagent 1 application solution are pre-warmed in advance at 37)

       

      Non-enzyme tube

      Enzyme tube

      1mmol/L GSH (mL)

      0.2

      0.2

      Sample (mL)

       

      0.1

      Pre-heat the tubes at 37 water bath for 5 min. Preheat Reagent 1 application solution at 37 for 5 min at the same time.

      Reagent 1 application solution (mL)

      0.1

      0.1

      React at 37 water bath for 5 min accurately.

      Reagent 2 (mL)

      2

      2

      Sample (mL)

      0.1

       

      Mix fully and centrifuge at 3100 g for 10 min, then take 1 mL of supernatant for chromogenic reaction.

       

      2)      Chromogenic reaction


       

      Blank tube

      Standard tube

      Non-enzyme tube

      Enzyme tube

      GSH standard application solution (mL)

      1

       

       

       

      20 μmol/L GSH standard solution (mL)

       

      1

       

       

      Supernatant (mL)

       

       

      1

      1

      Reagent 3 application solution (mL)

      1

      1

      1

      1

      Reagent 4 (mL)

      0.25

      0.25

      0.25

      0.25

      Reagent 5 application solution (mL)

      0.05

      0.05

      0.05

      0.05

      Mix fully and stand for 15 min at room temperature. Set to zero with double-distilled water and measure the OD values of each tube at 412 nm with 1 cm optical path quartz cuvette.

       

      Performance characteristics

      Technical parameter

      Detection range 12.65-387 U Average inter-assay CV 9.300000000000001%
      Sensitivity 12.65 U Average intra-assay CV 4.9%
      Average recovery rate 105%

      Citations

      1. Pinus Densiflora Bark Extract Prevents Selenite-induced Cataract Formation In The Lens Of Sprague Dawley Rat Pups.
        IF: 2.057
        Journal: Molecular Vision(2017)
        PMID: 28943754
        Products: E-EL-0026 E-EL-0060 E-BC-K096-S 
        Sample type: Tissue homogenate
        Reactivity: Rat
      2. The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers.
        IF: 2.885
        Journal: Journal of Diabetes Research(2018)
        DOI: 10.1155/2018/7341242
        PMID: 30009183
        Products: E-BC-K096-S E-BC-K020-M E-EL-0070 
        Reactivity: Rat
      3. Intraocular Pressure-Lowering Effects of Imidazo [1, 2-α]-and Pyrimido [1, 2-α] Benzimidazole Compounds in Rats with Dexamethasone-Induced Ocular Hypertension
        IF: 3.17
        Journal: European Journal of Pharmacology(2019)
        DOI: 10.1016/j.ejphar.2019.01.059
        PMID: 30716317
        Products: E-BC-K020-M E-BC-K096-S E-BC-K031-S 
        Sample type: Tissue homogenate
        Reactivity: Rat
      4. Supplementation of Short-Chain Fatty Acid, Sodium Propionate, in Patients on Maintenance Hemodialysis: Beneficial Effects on Inflammatory Parameters and Gut-Derived Uremic Toxins, A Pilot Study (PLAN Study)
        IF: 5.688
        Journal: Journal of Clinical Medicine(2018)
        DOI: 10.3390/jcm7100315
        PMID: 30274359
        Products: E-BC-K096-S 
        Sample type: Serum
        Reactivity: Human
      5. The Effects of Momordica Balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers
        IF: 3.04
        Journal: Journal of Diabetes Research(2018)
        DOI: 10.1155/2018/7341242
        PMID: 30009183
        Products: E-BC-K020-M E-BC-K096-S 
        Sample type: Tissue homogenate
        Reactivity: Rat
      6. Pinus Densiflora Bark Extract Prevents Selenite-induced Cataract Formation In The Lens Of Sprague Dawley Rat Pups
        IF: 2.174
        Journal: Molecular Vision(2017)
        PMID: 28943754
        Products: E-BC-K096-S 
        Sample type: Cataractous lenses
        Reactivity: Rat
      Show AllShorten
      • Show all (1)
      • Reviews (0)
      • Q&A (1)
      Q

      T****************gSubmitted [ Apr 23 2019 ]

      Asked: We don\'t have 412 nm of colorimetric spectro filter in my lab, we only have the 415 nm. And so, is it ok if we use 415nm? would it effect the accuracy of the measurement significantly? Could give me the contract of distributor in Thailand. Thank you.

      Reply

      A

      adminSubmitted [ Apr 23 2019 ]

      Answered: Dear Thapanee, Thanks for your message. It is ok to use 412nm to test the kit. Pls contact with the distributor to have the kit. Pacific Science Co.,Ltd. 62,64 Soi Charansanitwong 49/1 Charansanitwong rd. Bangbumru Bangplad Bangkok 10700 THAILAND Tel : +662-4330068-9 ext. 212 Email : pacscien@ksc.th.com Any questions, pls feel free to ask. Best regards,

      ... Show All Show Less
      MSDS for ELISA

      Browsing History


        People Also Bought

        Leave Message

        *
        *
        *
        *
        *
        *
        *
        Message submitted successfully !