Current Position:Home>>Kits>>Metabolism Assays>> Glutathione Peroxidase (GSH-Px) Activity Assay Kit
Cat.No.:E-BC-K096-S
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Protocols for metabolism assays
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Synonyms | GSH-PX |
Sample type | Serum,plasma,cells,cell culture supernatant,tissue |
Sensitivity | 12.65 U |
Detection range | 12.65-387 U |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 70 min |
Precision | Average inter-assay CV: 9.300%Average intra-assay CV: 4.900% |
Other instruments required | Micropipettor, Incubator, Vortex mixer, Centrifuge |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
A significant decline of GSH-Px activity due to ovariectomy (OVX) was observed (P=0.039). Treatment with annatto tocotrienol (ANTT) and Raloxifene (RAL) elevated GSH-Px activity.
N V Mohamad et al investigate the therapeutic effect of annatto tocotrienol for postmenopausal bone loss. Glutathione peroxidase (GSH-Px) activity of rat serum was determined using GSH-Px activity assay kit (E-BC-K096-S).
The optimal sampling volume are different for different species, also are different for different sample type. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the inhibition ratio is 20%~60% (the optimal inhibition ratio is the range of 45%~55%.) before formal experiment.
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Sample: plasma |
PMID: 35489465 |
Sample: pig testicular cell (ST cell) |
PMID: 34399203 |
Sample: heart |
DOI: 10.3390/nu14153179 |
|
Sample: hippocampus,cortex |
Sample: serum,ruminal fluid |
PMID: 34919900 |
DOI: 10.1155/2020/2978340 |
|
PMID: 32655765 |
Sample: Plasma,Urine,Tissue homogenate |
DOI: 10.3390/md20050308 |
|
Sample: Human dermal fibroblasts (HDFs) |
PMID: 33548266 |
Sample: Cell sample |
PMID: 36375957 |
Sample: larvae |
DOI: 10.3390/jcm7100315 |
|
PMID: 30274359 |
Sample: Serum |
PMID: 34769040 |
Sample: serum |
PMID: 36160397 |
Sample: primary cardiomyocyte |
Sample: testicular tissue |
Sample: brain tissue |
PMID: 35443217 |
Sample: serum |
PMID: 34454183 |
Sample: brain tissue,HT-22 cell |
PMID: 34684685 |
Sample: HDF cell |
PMID: 31279280 |
Sample: Tissue homogenate |
PMID: 31972360 |
Sample: Tissue homogenate |
PMID: 32570115 |
Sample: Cell sample |
Sample: HepG2 cell |
Sample: liver,kidney,lungs |
PMID: 35819692 |
Sample: PCC 6803 strains |
Sample: Tissue homogenate |
DOI: 10.3390/ani12233245 |
|
Sample: skimmed milk |
Sample: liver |
Sample: BEAS-2B cells |
PMID: 31161598 |
Sample: SH-SY5Y cells |
PMID: 36241955 |
Sample: serum |
PMID: 35058758 |
Sample: hippocampus tissue |
DOI: 10.1002/jat.4424 |
|
Sample: AC16 cells |
DOI: 10.1002/jsfa.12010 |
|
PMID: 35567370 |
Sample: serum |
PMID: 32097848 |
Sample: Plant Tissue |
DOI: 10.21037/atm-22-483 |
|
PMID: 35280364 |
Sample: myocardium |
PMID: 32438076 |
Sample: Tissue homogenate |
Sample: kidney tissue |
PMID: 28943754 |
Sample: lens tissue |
PMID: 29129047 |
Sample: Tissue homogenate |
DOI: 10.2147/IJGM.S402206 |
|
Sample: Serum |
PMID: 30820147 |
Sample: lens tissue |
Sample: liver |
DOI: 10.1155/2020/1936406 |
|
PMID: 32117470 |
Sample: Plasma,Tissue homogenate |
DOI: 10.1111/jphp.13319 |
|
Sample: Cell lysate |
DOI: 10.1002/jez.2603 |
|
Sample: cortex |
DOI: 10.1111/jfbc.14129 |
|
PMID: 35298033 |
Sample: gastric,jejunal, colonic |
DOI: 10.1111/jfbc.13865 |
|
PMID: 34263474 |
Sample: Liver,kidney |
PMID: 32689984 |
Sample: Plant Fluid |
DOI: 10.1155/2018/7341242 |
|
Sample: kidney tissues |
DOI: 10.3390/cimb45020057 |
|
Sample: colonic tissue |
PMID: 31927058 |
Sample: Tissue homogenate |
PMID: 29250160 |
Sample: Urine |
DOI: 10.1111/jfbc.13359 |
|
Sample: lung,liver,kidney |
PMID: 32744464 |
Sample: Tissue homogenate |
Sample: Serum |
DOI: 10.1155/2020/9046862 |
|
PMID: 32318290 |
Sample: Serum |
Sample: plasma |
L*mSubmitted [ Dec 17 2023 ]
Asked: From the manual Appendix II Sample Preparation part, for the cells sample, it is stated that (3) Treat the cells with ultrasonic cell disruptor (200 W, 2 s/time, interval for 3 s, the total time is 10 min). However, the ultrasonicator in our laboratory is QSONICA Q500 Sonicator which operating at 500 Watts, 20 kHz and 30% amplitude/intensity. May I ask if using this ultrasonicator parameter, what is the sonication duration (second/time), interval duration and total time you would recommended and required in order to lyse the HepG2 cells (around 1 x 10^6 / 500 µL) for GSH-Px detection in this assay?
Reply
adminSubmitted [ Dec 17 2023 ]
Answered: The ultrasonic cell disruptor parameters of broken cells given in our instructions are for reference only, and can be adjusted appropriately for different instruments. You can perform ultrasonicator with parameters commonly used in the laboratory, as long as the purpose of cell breakage is achieved.
A***********nSubmitted [ May 25 2023 ]
Asked: Hi, may I know that this kit is suitable using rat\'s liver for hepatoprotective test?
Reply
adminSubmitted [ May 25 2023 ]
Answered: Hi Amil Benjamin, E-BC-K096-M/S are suitalbe using rat's liver for hepatoprotective test.
S****************iSubmitted [ Mar 29 2023 ]
Asked: Can we use this kit for saliva
Reply
adminSubmitted [ Mar 29 2023 ]
Answered: Hello Shalini Pasupuleti,thank you for your support.This kit you inquire is suitable for saliva samples.
M*****aSubmitted [ Oct 14 2022 ]
Asked: We only look at the size where its mentioned 100 assays. So, could the kit be used for 100 samples?
Reply
adminSubmitted [ Oct 14 2022 ]
Answered: 100 Assays represents 100 times and 48 samples can be tested. 100Assays= 2 blank tubes +2 standard tubes + 48 non-enzyme tubes +48 enzyme tubes (each sample need a control)
M*****aSubmitted [ Sep 14 2022 ]
Asked: Can the kits detect cells that have been lysed in RIPA buffer
Reply
adminSubmitted [ Sep 14 2022 ]
Answered: Lysates are not recommended for enzymatic indexes, which may affect the determin. This kit has its own homogenate medium.
A***********fSubmitted [ Aug 30 2022 ]
Asked: Kindly respond, can we use 405nm spectrophotometry filter? Or alternatively can this be done in a microplate instead? Regards
Reply
adminSubmitted [ Aug 30 2022 ]
Answered: The optimal detection wavelength is 412nm for this kit. There may be a little error if using 405nm spectrophotometry filter. The whole trend of samples will be ok. 405nm spectrophotometry filter could be a choice if you don't have 412nm spectrophotometry filter. It couldn't be used in a microplate reader.
F**oSubmitted [ Dec 19 2020 ]
Asked: I confused about the blank tube. If I have double beam spektro, what solution do i use for zeroing? And what blank for the standard & sample ?
Reply
adminSubmitted [ Dec 19 2020 ]
Answered: Hi Faro, Thanks for your message. Could you let us know which country you are from, so that I could forward to our local sales representative to contact you ASAP. Best regards
T****************gSubmitted [ Apr 23 2019 ]
Asked: We don\'t have 412 nm of colorimetric spectro filter in my lab, we only have the 415 nm. And so, is it ok if we use 415nm? would it effect the accuracy of the measurement significantly? Could give me the contract of distributor in Thailand. Thank you.
Reply
adminSubmitted [ Apr 23 2019 ]
Answered: Dear Thapanee, Thanks for your message. It is ok to use 412nm to test the kit. Pls contact with the distributor to have the kit. Pacific Science Co.,Ltd. 62,64 Soi Charansanitwong 49/1 Charansanitwong rd. Bangbumru Bangplad Bangkok 10700 THAILAND Tel : +662-4330068-9 ext. 212 Email : pacscien@ksc.th.com Any questions, pls feel free to ask. Best regards,