Reduced Glutathione (GSH) Colorimetric Assay Kit (E-BC-K030-S)
For research use only.
| Detection Principle | Reduced glutathione (GSH) can react with dithionitrobenzoic acid (DTNB) to produce thio-nitrobenzoic acid and glutathione disulfide. Nitromercaptobenzoic acid is a yellow compound which has the maximum absorption peak at 420 nm. The GSH content can be calculated by measuring the OD value at 420 nm. |
| Synonyms | GSH |
| Sample Type | Serum,Plasma,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (420 nm) |
| Research Area | Oxidative Stress , Cuproptosis |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.26 mg GSH/L |
| Detection Range | 0.26-122.8 mgGSH/L |
| Precision | inter-assay CV: 2.4% | intra-assay CV: 1.8% |
| Sample Volume | 0.7 mL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1 |
| 10% Mouse brain tissue homogenization | 1 |
| 10% Rat liver tissue homogenization | 1 |
| Human plasma | 1 |
| Rat plasma | 1 |
| 10% Carrot tissue homogenization | 1 |
| 293T supernatant | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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