Glycerol Colorimetric Assay Kit (E-BC-K340-M)
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For research use only.
| Detection Principle | Glycerol is transformed by enzyme to produce hydrogen peroxide, which is catalyzed by oxidase in the presence of 4-amino-antipyrprid and phenol to produce red quinones, the color of which is proportional to the content of glycerol. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500-520 nm, optimum wavelength: 510 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Energy Metabolism |
| Other Reagents Required | Double distilled water, Isopropanol (AR), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.01 mmol/L |
| Detection Range | 0.01-1.00 mmol/L |
| Precision | inter-assay CV: 8% | intra-assay CV: 4% |
| Sample Volume | 10 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 2 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 1 |
| Cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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