Glycogen Colorimetric Assay Kit (Liver/Muscle Samples) (E-BC-K073-S)
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For research use only.
| Detection Principle | Under the presence of concentrated sulfuric acid, glycogen can be dehydrated to furfural derivatives. Furfural derivatives can form blue compound with anthracenone. The concentration of the compound can be measured by colorimetric quantification at 620 nm with glucose standard buffer of same treatment. Glycogen is quite stable in concentrated alkali solution. Heating the tissue sample in concentrated alkali solution before color development will remove other components and keep the glycogen. |
| Sample Type | Animal liver,Muscle |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (620 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), Concentrated sulfuric acid |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.80 mg/g liver tiss |
| Detection Range | 1.80-180 mg/g liver |
| Precision | inter-assay CV: 7.3% | intra-assay CV: 3.7% |
| Sample Volume | 100 μL(tissue homogenate) |
| Assay Time | 50 min |
| Cat.No. | Product Name | Clone No. |
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