Glycogen Colorimetric Assay Kit (Liver/Muscle Samples)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K073-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $200
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(620 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      Under the presence of concentrated sulfuric acid, glycogen can be dehydrated to furfural derivatives. Furfural derivatives can form blue compound with anthracenone. The concentration of the compound can be measured by colorimetric quantification at 620 nm with glucose standard buffer of same treatment. Glycogen is quite stable in concentrated alkali solution. Heating the tissue sample in concentrated alkali solution before color development will remove other components and keep the glycogen.

      Performance characteristics

      Sample type animal liver,muscle
      Sensitivity 1.80 mg/g liver tiss
      Detection range 1.80-180 mg/g liver
      Precision Average inter-assay CV: 7.3%Average intra-assay CV: 3.7%
      Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge
      Other reagents required Normal saline (0.9% NaCl), Concentrated sulfuric acid
      Storage 2-8℃
      Valid period 6 months
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      MSDS for ELISA ISO 9001

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