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GNAZ Polyclonal Antibody

Cat:E-AB-10330
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human ;Mouse ;Rat
  • Applications: WB
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Product Details
Verified Samples Verified Samples in WB:Human fetal brain,Human brain malignant glioma,Mouse brain
Dilution

WB 1:500-1:2000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant protein of human GNAZ
Abbre GNAZ
Synonyms G(x) alpha chain;GNAZ;GNAZ;Guanine nucleotide binding protein (G protein) alpha z polypeptide;Guanine nucleotide binding protein G(z) alpha subunit G(x) alpha chain;Guanine nucleotide binding protein G(z) subunit alpha;Guanine nucleotide-binding protein G(z) subunit alpha;Gz alpha;Gz-alpha;Human Gx alpha gene;Transducin alpha
Swissprot
Calculated MW 41kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Membrane.
Concentration 0.4 mg/mL
Buffer PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Purification Method Affinity purification
Research Areas Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background Guanine nucleotide-binding protein G(z) subunit alphais aproteinthat in humans is encoded by theGNAZgene. The protein encoded by this gene is a member of a G protein subfamily that mediates signal transduction in pertussis toxin-insensitive systems. This encoded protein may play a role in maintaining the ionic balance of perilymphatic and endolymphatic cochlear fluids. GNAZ has been shown to interact with EYA2, RGS20 and RGS19.