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GSH(Glutathione) ELISA Kit

  • Cat.No.:E-EL-0026

  • Reactivity: Universal

To Purchase E-EL-0026

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 1.56-100 μg/mL
Sensitivity 0.94 μg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes GSH in samples. No significant cross-reactivity or interference between GSH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of GSH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GSH. During the reaction, GSH in the sample or standard competes with a fixed amount of GSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GSH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GSH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level GSH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level GSH were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (μg/mL) 5.06 13.81 43.81 5.19 13.60 42.40
Standard deviation 0.27 0.82 2.24 0.27 0.66 1.59
CV (%) 5.34 5.94 5.11 5.20 4.85 3.74

Recovery

The recovery of GSH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-101 94
EDTA plasma(n=8) 88-101 96
Cell culture media(n=8) 86-99 93

Target Information

SynonymsGSH

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. European Journal of Neuroscience (2020) IF: 3.115
    Neuroprotective effects of brain-derived neurotrophic factor against amyloid beta 1-40-induced retinal and optic nerve damage

    DOI: 10.1111/ejn.14662

    PMID: 31883161

    Sample: Tissue homogenate
  2. Inflammopharmacology (2020) IF: 3.238
    Perindopril ameliorates experimental Alzheimer's disease progression: role of amyloid β degradation, central estrogen receptor and hyperlipidemic-lipid raft signaling

    DOI: 10.1007/s10787-020-00724-4

    PMID: 32488543

    Sample: Serum,Tissue homogenate
  3. Scientific Reports (2020) IF: 3.998
    Reproductive effects of subchronic exposure to acetamiprid in male rats

    DOI: 10.1038/s41598-020-65887-0

    PMID: 32488017

    Sample: Plasma,Tissue homogenate
  4. Gastroenterology Report (2020) IF: 2.96
    Effect of Helicobacter pylori-eradication therapy on hepatic steatosis in patients with non-alcoholic fatty liver disease: a randomized-controlled pilot study

    DOI: 10.1093/gastro/goz058

    PMID: 32499918

    Sample: Serum
  5. Molecular vision (2019) IF: 2.219
    Polyphenol-enriched fraction of Vaccinium uliginosum L. protects selenite-induced cataract formation in the lens of Sprague-Dawley rat pups

    DOI: 99999999

    PMID: 30820147

    Sample: Tissue homogenate
  6. Neurochemical Research (2017) IF: 2.581
    Antioxidative And Anti-Apoptotic Roles Of Silibinin In Reversing Learning And Memory Deficits In APP/PS1 Mice.

    DOI: 10.1007/s11064-017-2389-3

    PMID: 28852940

    Sample: Tissue homogenate
  7. Molecular Vision (2017) IF: 2.174
    Pinus Densiflora Bark Extract Prevents Selenite-induced Cataract Formation In The Lens Of Sprague Dawley Rat Pups.

    PMID: 28943754

    Sample: Tissue homogenate
  8. The Korean Journal of Physiology & Pharmacology: Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology (2020) IF: 1.805
    The hepato-protective effect of eupatilin on an alcoholic liver disease model of rats

    DOI: 10.4196/kjpp.2020.24.5.385

    PMID: 32830145

    Sample: Tissue homogenate
  9. Andrologia (2020) IF: 1.951
    Androgenic effect of aqueous leaf extract of Moringa oleifera on Leydig TM3 cells in vitro

    DOI: 10.1111/and.13825

    PMID: 32926461

    Sample: Cell lysate
  10. Asian J Pharm Clin Res (2019)
    PROTECTIVE EFFECT OF ZILEUTON AND MK-866 AGAINST HEPATIC DAMAGE INDUCED BY DOXORUBICIN

    DOI: 10.22159/ajpcr.2019.v12i2.30815

    PMID: 99999999

    Sample: Serum
  11. Proff. Sameerah Ahmed (2020)
    Study the Effect of Antioxidants and Oxidative Products and Their Genetic Effect on Methionine Synthase in People with Autism Spectrum Disorder

    DOI: 10.2139/ssrn.3559385

    PMID: 99999999

    Sample: Plasma
  12. Mustansiriya Medical Journal (2018)
    Evaluation of the effects of TAK-242 and GIT-27 on methotrexate-induced liver injury

    DOI: 10.4103/MJ.MJ_15_18

    PMID: 99999999

    Sample: Serum
  13. Journal of Pharmaceutical Sciences and Research (2018)
    Protective effect of montelukast against acute kidney injury in rats induced by diclofenac

    DOI: 99999999

    PMID: 99999999

    Sample: Serum
  14. Journal of Pharmaceutical Sciences and Research (2019)
    The Effect of Vitamin D and Co-enzyme Q10 Replacement Therapy on Hormonal Profile and Ovulation Statusin Women with Clomiphene Citrate Resistant Polycystic Ovary Syndrome

    DOI: 99999999

    PMID: 99999999

    Sample: Serum
  15. Al-Kufa University Journal for Biology (2019)
    Detection of some antioxidant markers in saliva of patients with beta thalassemia major

    DOI: 99999999

    PMID: 99999999

    Sample: Serum,Saliva
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