Synonyms:Glycogen Synthase Kinase 3 Beta,Glycogen synthase kinase-3 beta,GSK 3 beta,GSK-3 beta,GSK3B,GSK3B,GSK3beta isoform,Serine/threonine-protein kinase GSK3B
|Synonyms||Glycogen Synthase Kinase 3 Beta,Glycogen synthase kinase-3 beta,GSK 3 beta,GSK-3 beta,GSK3B,GSK3B,GSK3beta isoform,Serine/threonine-protein kinase GSK3B|
|Immunogen||Synthetic Peptide of GSK3β|
|Application||WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R217)|
|Recommended dilution||WB 1:1000-2000, IHC 1:100-200|
|Cellular localization||Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.|
|Tissue specificity||Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.|
|Purification||Protein A purification|
|Storage instructions||Store at -20℃. Avoid freeze / thaw cycles.|
|Storage buffer||PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4|
|Background||Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.|
Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain using GSK3 beta Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using GSK3 beta Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Human stomach carcinoma tissue using GSK3 beta Monoclonal Antibody at dilution of 1:200.