H+K+-ATPase Activity Assay Kit

    • Biochemical Assay Kit-Elabscience
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    >
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K122-S

      Size:
      • 100Assays
      • 200Assays
      Qty:
      - +
      Price: $180
      Can detect 50 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(660 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      ATPase can decompose ATP to produce ADP and inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. The inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium molybdate compound, which is reduced with reducing agent to form molybdenum blue, and has absorption peak at 660 nm. Determine the concentration of molybdenum blue to calculate the amount of inorganic phosphorus.

      Performance characteristics

      Sample type animal tissue,cells
      Precision Average inter-assay CV: 9.8%Average intra-assay CV: 4.4%
      Other instruments required Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer
      Other reagents required Normal saline (0.9% NaCl)
      Storage Reagent 4: -20℃, others: 2-8℃
      Valid period 6 months

      Dilution of sample

      Dilute the 10% tissue homogenate to different concentrations of 2%, 1%, and 0.5%, then take 100 μL for pre-experiment according to the operation steps. The OD value of sample tube should be less than 1.0.

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      10% Animal tissue homogenate

      5

      GES-1 cells (2.52mg/mL)

      1

      Note: The diluent is normal saline (0.9% NaCl).
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      MSDS for ELISA ISO 9001

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