ATPase can decompose ATP to produce ADP and inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. The inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium molybdate compound, which is reduced with reducing agent to form molybdenum blue, and has absorption peak at 660 nm. Determine the concentration of molybdenum blue to calculate the amount of inorganic phosphorus.

Performance characteristics

Sample type	Animal tissue,cells
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	60 min
Precision	Average inter-assay CV: 9.800%Average intra-assay CV: 4.400%
Other instruments required	Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl)
Storage	Reagent 4: -20℃, others: 2-8℃
Valid period	12 months

Images

The activity of H+/K+-ATPase was significantly increased by ethanol and reduced in treating groups. (###P<0.001 vs saline group, ***P<0.001 with ethanol group)

M Noman et al investigate the antioxidant and anti-inflammation capacity of brucine. H+/K+-ATPase activity of rat gastric was determined using H+/K+-ATPase activity assay kit (E-BC-K122-S).

Dilution of sample

Dilute the 10% tissue homogenate to different concentrations of 2%, 1%, and 0.5%, then take 100 μL for pre-experiment according to the operation steps. The OD value of sample tube should be less than 1.0.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Animal tissue homogenate	5
GES-1 cells (2.52mg/mL)	1

Note: The diluent is normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

INTERNATIONAL JOURNAL OF PHARMACEUTICS (2021) IF: 5.875
Famotidine-loaded solid self-nanoemulsifying drug delivery system demonstrates exceptional efficiency in amelioration of peptic ulcer

DOI: 10.1016/j.ijpharm.2021.121303

PMID: 34798155
Sample: stomach
Frontiers in Pharmacology (2022) IF: 5.988
Pharmacological investigation of brucine anti-ulcer potential

DOI: 10.3389/fphar.2022.886433

PMID: 36059979
Sample: stomach tissue
Frontiers in Pharmacology (2022) IF: 5.988
Pharmacological basis of bergapten in gastrointestinal diseases focusing on H+/K+ ATPase and voltage-gated calcium channel inhibition: A toxicological evaluation on vital organs

DOI: 10.3389/fphar.2022.1005154

Sample: stomach tissue
Frontiers in Pharmacology (2022) IF: 5.988
Effect of Rumex dentatus on Gastrointestinal Protection and Toxicology in Rodents via Investigating H⁺/K⁺-ATPase, Calcium Channels, and PDE Mediated Signaling.

DOI: 10.3389/fphar.2022.936161

Sample: Stomach tissue
MOLECULES (2022) IF: 4.927
Pharmacological Basis of Rumex hastatus D. Don in Gastrointestinal Diseases with Focusing Effects on H+/K+-ATPase, Calcium Channels Inhibition and PDE Mediated Signaling: Toxicological Evaluation on Vital Organs

DOI: 10.3390/molecules27185919

PMID: 36144661
Sample: stomach tissue
MOLECULES (2022) IF: 4.927
Novel Isoxazole Derivative Attenuates Ethanol-Induced Gastric Mucosal Injury through Inhibition of H+/K+-ATPase Pump, Oxidative Stress and Inflammatory Pathways

DOI: 10.3390/molecules27165065

PMID: 36014311
Sample: Stomach tissue
BMC Pharmacology & Toxicology (2022) IF: 2.605
Pharmacological evaluation of newly synthesized organotin IV complex for antiulcer potential

DOI: 10.1186/s40360-022-00596-0

PMID: 35906691
Sample: gastric tissue

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Reviews/Q&A

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P****aSubmitted [ Dec 13 2022 ]

Asked: Can you tell me how long will it take for the Product H+K+ATPase assay kit (Cat.No.:E-BC-K122-S) to be delivered to India (Zip 781017). Please tell me also about the total cost involved.

adminSubmitted [ Dec 13 2022 ]

Answered: We will reply you by E-mail.

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