The Ham's F-12K medium increases the concentration of amino acids and pyruvic acid on the basis of Ham's F-12, reduces the concentration of glucose, and modifies the composition and content of the salt.The Ham's F-12K medium was initially designed to culture primary human hepatocytes and differentiated cells of rats and chickens. The Ham's F-12K medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

General Information

Product Form	Liquid
Concentration	1×
pH	7.2-7.4
D-Glucose	1260mg/L
HEPES	Negative
L-glutamine	2mM
NaHCO₃	2500mg/L
Phenol red	3mg/L
Storage	2-8℃ ,Shading Light
Shipping	RT
Expiration date	24 months

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Notes

1. This product is for research use only.;
2. This product is sterilized by 0.1μm filtration.;
3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture.

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

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