Hematoxylin Staining Buffer

Introduction

Hematoxylin Staining Buffer synthesizes many classical methods of hematoxylin staining, such as Harris method, Mayer method, etc., which simplifies the operation steps, shortens the operation time, and does not use toxic reagents such as mercury and methanol in the staining buffer. It can be used for staining of tissue slice or cultured cells.

Hematoxylin staining, also known as hematoxylin staining, is one of the most commonly staining methods for tissues and cells. There are many different ways to prepare hematoxylin staining buffer. Different methods can stain the nucleus into different shades of blue or blue purple.

The nucleus is blue when it is stained by this product.                                          

Components

Reagents Preparation

1. Fixative solution: 4% Paraformaldehyde (It is recommended to use Elabscience^® E-IR-R113).

2. Differentiation solution: 5% acetic acid solution or 0.5% hydrochloric alcohol.

3. If dehydration, transparency and sealing treatment are needed. Xylene, Neutral Balsam (It is recommended to use Elabscience^® E-IR-R118), 80% ethanol, 90% ethanol and anhydrous ethanol should be prepared.

4. 70% ethanol.

5. Preparation of paraffin section. Xylene and gradient ethanol

Storage

Store at room temperature for 12 months.

Cautions

1. It is recommended to differentiate, which will make the nuclear staining more clearly.

2.The dye solution can be reused many times. When the effect is not good, the new dye solution can be replaced.

3. It is recommended to take 1~2 slices for pre-experiment.

4.This product is for research use only. It couldn’t be used for clinical diagnosis or treatment, food or medicine, and can’t be stored in residence.

5. For your safety and health, please wear the lab coat and disposable gloves before the experiments.