HSP27 Polyclonal Antibody
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Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB;IHC-p;IF
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:22RV1 Verified Samples in IHC:Human stomach cancer Verified Samples in IF:Mouse kidney |
Dilution |
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Synthesized peptide derived from human HSP27 around the non-phosphorylation site of Ser15. |
Abbre | HSP27 |
Synonyms | Heat shock 27kDa protein;28 kDa heat shock protein;CMT2F;DKFZp586P1322;epididymis secretory protein Li 102;Estrogen regulated 24 kDa protein;Estrogen-regulated 24 kDa protein;Heat shock 25kDa protein 1;Heat shock 27 kDa protein;Heat shock 27kD protein 1;Heat shock 27kDa protein 1;Heat shock 28kDa protein 1;Heat Shock Protein 27;Heat shock protein beta 1;Heat shock protein beta-1;heat shock protein family B (small) member 1;HEL-S-102;HMN2B;HS.76067;Hsp 25;HSP 27;Hsp 28;Hsp B1;Hsp25;HSP27;Hsp28;HspB1;HSPB1;SRP27;Stress responsive protein 27;Stress-responsive protein 27 |
Swissprot | |
Calculated MW | 23kDa |
Observed MW |
27kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cytoplasm. Nucleus. Cytoplasm>cytoskeleton>spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. |
Concentration | 1 mg/mL |
Buffer | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4 |
Purification Method | Affinity purification |
Research Areas | Cancer; Cardiovascular; Signal Transduction |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | Involved in stress resistance and actin organization. |
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Sesamol-Loaded PLGA Nanosuspension for Accelerating Wound Healing in Diabetic Foot Ulcer in Rats
IF:5.115
Journal:International Journal of Nanomedicine
PMID:33262587