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HSP90 alpha Monoclonal Antibody

Cat:E-AB-22117
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Mouse
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC-p
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Product Details
Verified Samples Verified Samples in WB:Hela,Mouse brain,Rat brain
Verified Samples in IHC:Human colon carcinoma
Dilution

WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:40000

Western Blot Operation Guide
Clonality Monoclonal
Immunogen Synthesized peptide derived from the C-terminal region of human HSP90A
Abbre HSP90 α
Synonyms EL52;epididymis luminal secretory protein 52;Heat shock 86 kDa;heat shock 90kD protein 1;alpha;Heat shock 90kD protein 1;alpha like 4;heat shock 90kD protein;alpha-like 4;Heat shock 90kDa protein 1 alpha;Heat shock protein 90kDa alpha (cytosolic) class A member 1;Heat shock protein HSP 90-alpha;HS90A;HSP 86;HSP86;Hsp89;HSP89A;Hsp90;HSP90A;HSP90AA1;HSP90ALPHA;HSP90N;HSPC1;HSPCA;HSPCAL1;HSPCAL4;HSPN;LAP 2;LAP2;lipopolysaccharide-associated protein 2;LPS-associated protein 2;Renal carcinoma antigen NY-REN-38
Swissprot
Observed MW 95kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Protein A purification
Research Areas Cancer; Signal Transduction
Clone No. Clone:5D4
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Molecular chaperone. Has ATPase activity.