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Human AD(Adropin) ELISA Kit

  • Cat.No.:E-EL-H5307

  • Reactivity: Human

To Purchase E-EL-H5307

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 12.50-800 pg/mL
Sensitivity 7.50 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human AD in samples. No significant cross-reactivity or interference between Human AD and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human AD concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AD. You can calculate the concentration of Human AD in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human AD were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human AD were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 43.68 95.22 350.77 41.00 89.88 330.27
Standard deviation 2.97 3.93 16.77 2.85 3.79 14.47
CV (%) 6.80 4.13 4.78 6.95 4.22 4.38


The recovery of Human AD spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-104 97
EDTA plasma (n=8) 89-102 95
Cell culture media (n=8) 89-101 94


Samples were spiked with high concentrations of Human AD and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q6UWT2
SynonymsENHO, Energy Homeostasis Associated
Research AreaCardiovascular, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Biomedicines (2023) IF: 4.757
    The Impact of SGLT2 Inhibitor Dapagliflozin on Adropin Serum Levels in Men and Women with Type 2 Diabetes Mellitus and Chronic Heart Failure

    DOI: 10.3390/biomedicines11020457

    Sample: serum
    Association between adropin and coronary artery lesions in children with Kawasaki disease

    DOI: 10.1007/s00431-021-03977-5

    PMID: 33712900

    Sample: serum
  3. Diagnostics (2022) IF: 3.992
    Interplay between Myokine Profile and Glycemic Control in Type 2 Diabetes Mellitus Patients with Heart Failure

    DOI: 10.3390/diagnostics12122940

    Sample: serum
    A novel biomarker in patients with knee osteoarthritis: adropin

    DOI: 10.1007/s10067-018-4052-z

    Sample: Serum
    Serum Adropin Levels Are Reduced in Adult Patients with Nonalcoholic Fatty Liver Disease

    DOI: 10.1159/000500106

    Sample: serum
  6. Anatolian Journal of Cardiology (2019) IF: 1.112
    Association between serum adropin levels and isolated coronary artery ectasia in patients with stable angina pectoris

    DOI: 10.14744/AnatolJCardiol.2019.90349

    Sample: serum
  7. Canadian Respiratory Journal (2020) IF: 1.639
    Alterations in Serum Adropin, Adiponectin, and Proinflammatory Cytokine Levels in OSAS

    DOI: 10.1155/2020/2571283

    PMID: 32454912

    Sample: Serum
    Serum irisin and adropin levels may be predictors for coronary artery ectasia

    DOI: 10.1080/10641963.2021.2018601

    PMID: 34994255

    Sample: serum
  9. Journal of Cancer Prevention (2018)
    Plasma Adropin as a Potential Marker Predicting Obesity and Obesity-associated Cancer in Korean Patients With Type 2 Diabetes Mellitus

    DOI: 10.15430/JCP.2018.23.4.191

    PMID: 30671402

    Sample: Plasma
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