Click here to view cell apoptosis assay products

Human Ang-Ⅱ(Angiotensin Ⅱ) ELISA Kit

  • Cat.No.:E-EL-H0326

  • Reactivity: Human

To Purchase E-EL-H0326

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes Ang-Ⅱ in samples. No significant cross-reactivity or interference between Ang-Ⅱ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Ang-Ⅱ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang-Ⅱ. During the reaction, Ang-Ⅱ in the sample or standard competes with a fixed amount of Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Ang-Ⅱ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Ang-Ⅱ were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 101.10 272.50 948.90 108.40 291.70 898.00
Standard deviation 6.70 14.40 43.60 6.60 14.60 44.00
CV (%) 6.63 5.28 4.59 6.09 5.01 4.90


The recovery of Ang-Ⅱ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-103 95
EDTA plasma(n=8) 95-107 101
Cell culture media(n=8) 91-105 96


Samples were spiked with high concentrations of Human Ang-Ⅱ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P01019
Research AreaCancer, Cardiovascular, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

    HMGB1, NLRP3, IL-6 and ACE2 levels are elevated in COVID-19 with headache: a window to the infection-related headache mechanism

    DOI: 10.1186/s10194-021-01306-7

    PMID: 34384355

    Sample: serum
  2. Frontiers in Endocrinology (2022) IF: 6.055
    Children With Short Stature Display Reduced ACE2 Expression in Peripheral Blood Mononuclear Cells

    DOI: 10.3389/fendo.2022.912064

    PMID: 35909539

    Sample: serum
    Metformin Alleviates Epirubicin-Induced Endothelial Impairment by Restoring Mitochondrial Homeostasis

    DOI: 10.3390/ijms24010343

    Sample: Human Umbilical Vein Endothelial Cells (HUVECs)
  4. Journal of Translational Medicine (2021) IF: 5.531
    Pulmonary midkine inhibition ameliorates sepsis induced lung injury

    DOI: 10.1186/s12967-021-02755-z

    PMID: 33639987

    Sample: Plasma
  5. Frontiers in Pharmacology (2022) IF: 5.811
    Blocking VCAM-1 Prevents Angiotensin II-Induced Hypertension and Vascular Remodeling in Mice.

    DOI: 10.3389/fphar.2022.825459

    Sample: serum
    Type 1 diabetes is associated with significant changes of ACE and ACE2 expression in peripheral blood mononuclear cells

    DOI: 10.1016/j.numecd.2022.01.029

    PMID: 35260304

    Sample: peripheral blood mononuclear cell
    Sex Differences in Proatherogenic Cytokine Levels

    DOI: 10.3390/ijms21113861

    PMID: 32485823

    Sample: Serum
  8. Computational and Mathematical Methods in Medicine (2022) IF: 2.238
    Angiotensin II Mediates Cardiomyocyte Hypertrophy in Atrial Cardiomyopathy via Epigenetic Transcriptional Regulation

    DOI: 10.1155/2022/6312100

    PMID: 35756425

    Sample: heart tissue
Show AllShorten


  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
*When will you use it?