Human β-CTx(Beta Crosslaps) ELISA Kit
b-CTx、bCTXI、bCTX-I、B-Cr、BCL、Type I Collagen C-Telopeptide-Related Fraction
Price: $ 609
Price: $ 487
Price: $ 150
Price: Inquire
Price: Inquire
- Reactivity: Human
- Detection Range: 125-8000 pg/mL
- Sensitivity: 75 pg/mL
For research use only. Order now, ship in 3 days
Test Principle | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human β-CTx. During the reaction, Human β-CTx in the sample or standard competes with a fixed amount of Human β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human β-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human β-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve. |
Assay Type | Competitive-ELISA |
size | 96T / 48T / 24T / 96T*10 / 96T*5 |
Assay Time | 2.5h |
Detection Method | Colormetric |
Detection Range | 125-8000 pg/mL |
Sensitivity | 75 pg/mL |
Sample Volume | 50μL |
Sample Type | serum, plasma and other biological fluids |
Specificity | This kit recognizes Human β-CTx in samples.No significant cross-reactivity or interference between Human β-CTx and analogues was observed |
Precision | Both intra-CV and inter-CV are < 10%. |
Recovery | 80%-120% |
Introduction | This ELISA kit applies to the in vitro quantitative determination of Human β-CTx concentrations in serum, plasma and other biological fluids. |
Applications | ELISA |
Storage | 2-8℃/-20℃ |
Database | SwissProt: P02452 |
Syonoyms | b-CTx,bCTXI,bCTX-I,B-Cr,BCL,Type I Collagen C-Telopeptide-Related Fraction |
Research Area | Signal Transduction , Metabolism |
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Q1:Are human CTx I and human beta-CTX the same index?
These two items are different, and E-EL-H0835 detects 1196-1218aa of Human CTXⅠ; E-EL-H0960 detects 1207-1214aa of Human β-CTx. In addition, the functions of the two are different. CTXⅠ is the degradation indicator of type I collagen. β-CTx was the evaluation index of bone reresorption.
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Q2:How to operate the "repeated freeze-thaw" process mentioned in tissue or cell sample processing?
Tissue sample: After finishing tissue grinding, place it at -80℃ for 1h/ liquid nitrogen for 0.5h, and then gently shake it in a water bath at 30℃ to melt it quickly. Repeat this operation 1-2 times. Cell sample: Repeat the above freeze-thaw operation 2-3 times. If it is a membrane protein, it can be appropriately sonicated, but the temperature and frequency of ultrasound need to be controlled. It is recommended to add protease inhibitors to the sample in advance. PMSF(Cat.E-EL-SR002) is generally recommended.
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Q3:Can the standard curve in the manual be used directly for calculation?
The standard curve on the manual is an indicator curve, mainly used to show the shape of the standard curve after fitting and the quality control range of the highest/lowest OD value, you can not directly use. In addition, due to the influence of experimental operation, experimental environment, instrument parameter setting and other factors, the OD value of your actual standard curve may not be completely consistent with the instructions (overall high or low). In this case, it is recommended to control the first 4 blue wells in the color development stage with obvious color gradient and the maximum OD value of the standard curve above 1.2. If the correlation coefficient of the standard tune is above 0.99, it can be used as an effective standard curve.
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Q4:The sample/reagent volume is not enough, can I reduce the sample volume uniformly?
Neither can be reduced. The sample volume of each step should be strictly in accordance with the instructions, otherwise the experimental system will be changed, and the results will be inaccurate. If the customer really does not have enough sample volume, you can consult the technology to confirm whether the sample can be diluted, and judge the sample concentration through pre-experiment Degree situation. If the volume of the reagent is not enough, the number of holes to be tested can only be reduced according to the actual volume, and the detection can not be diluted.
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Q5:How long was the ELISA test?
Traditional Competion Method: about 2.0 h; Traditiona three-steps Sandwich method: about 3.5h; QuicKey ELISA: about 2.5h; QuicKey Pro ELISA: about 1.5h.
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Q6:Does ELISA require technical replication? How to set the positive or negative control?
It is recommended that customers do technical repetition, on the one hand can confirm the operation method, on the other hand can verify and improve the accuracy of the experimental results. A positive control in an ELISA test can be considered the standard product. Negative control is a blank matrix or a matrix solution with known low concentration that has homology and homogeneity with the object to be picked up, which is generally difficult to obtain, and can be set as a negative control if available. The routine is to set a blank control, that is, a sample diluent.
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Q7:Why should a product with two item numbers of a target test different sample types?
Different sample types of matrix are different, in order to reduce the influence of the matrix itself and improve the accuracy of routine sample measurement, usually set different systems of ELISA kits, and display with different product numbers. In addition, the concentration of some indicators varies greatly between different samples, so different products are used to meet customers' needs for ease of operation.
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Q8:How can I tell if my sample needs to be diluted or processed?
The detection range of the kit is not the same as the concentration range of the substance to be measured in the sample. It is recommended to estimate the concentration of the substance to be measured in the sample through relevant literature before the experiment and determine the actual concentration of the sample through pre-experiment. If it is a routine sample type, you can consult ELISA technical support to obtain the recommended dilution of the sample and schedule pre-laboratory testing. If the concentration of the substance to be measured in the sample is too high or too low, dilute or concentrate the sample appropriately. If the concentration of analyte in the sample is much lower than the minimum detection line of the kit, it is recommended to choose a kit with higher sensitivity for detection.
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Q9:Is it necessary to set up multiple Wells for ELISA?
In order to ensure the accuracy of the experimental results, we suggest that both standards and samples should be tested in duplicate. Of course, ELISA experiments do not necessarily have to be repeated /3 repeated Wells, customers can set the repeat or not according to their own needs.
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Q10:How to perform ultrasonic processing?
Using ultrasonic cell crusher, ultrasonic treatment of the suspension to lyse the cells (reference: Soniprep150 ultrasonic generator, ultrasonic treatment of 30s at an amplitude of 14μm, cell fragmentation; Or use an ultrasonic crusher, 200W, 2s/ times, gap 3s, total time 3-5min or 400 amp, 5s/ times, gap 10s, repeated 3-5 times), and then centrifuge at 2 to 8℃ at 1500×g for 10min, remove the cell debris, collect the supernatant. Attention should be paid to temperature during ultrasound.