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Human β-EP(Beta-Endorphin) ELISA Kit

Citations(4)Uniprot : P01189

Cat.No.:E-EL-H0572
Reactivity: Human

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes β-EP in samples. No significant cross-reactivity or interference between β-EP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of β-EP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with β-EP. During the reaction, β-EP in the sample or standard competes with a fixed amount of β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level β-EP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level β-EP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	45.50	153.00	435.30	46.70	158.10	426.00
Standard deviation	2.90	7.50	20.00	2.80	6.60	13.60
CV (%)	6.37	4.90	4.59	6.00	4.17	3.19

Recovery

The recovery of β-EP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-107	97
EDTA plasma(n=8)	87-102	93
Cell culture media(n=8)	84-97	91

Linearity

Samples were spiked with high concentrations of Human β-EP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P01189
Synonyms	b-EP

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Translational psychiatry (2018) IF: 4.691

Prefrontal expectancy and reinforcement-driven antidepressant placebo effects

DOI: 10.1038/s41398-018-0263-y
PMID: 30323205	Sample: Plasma

Acta Pharmacologica Sinica volume (2019) IF: 4.01

Sunny walking counts more

DOI: 10.1038/s41401-019-0241-9
PMID: 31249388	Sample: Urine

Acta Pharmacologica Sinica (2019) IF: 4.01

Low-intensity walking as mild medication for pressure control in prehypertensive and hypertensive subjects: how far shall we wander?

DOI: 10.1038/s41401-018-0202-8
PMID: 30760834	Sample: Urine

Mediators of Inflammation (2015) IF: 3.232

I-Gel Laryngeal Mask Airway Combined With Tracheal Intubation Attenuate Systemic Stress Response In Patients Undergoing Posterior Fossa Surgery.

DOI: 10.1155/2015/965925
PMID: 26273146	Sample: Serum

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Reviews/Q&A

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Reviews
Q&A

D********aSubmitted [ Mar 25 2018 ]

Asked: How long after receiving this kit will it expire?

P*******nSubmitted [ Mar 25 2018 ]

Answered: Dear Dr. Jessica, Thanks for your message. The valid time is 6 months after delivery. If you have ordered our products, please open the box, and you could find out the specific expiry date on reagent vials. At the same time, please notice the storage method for each vial in the kit.

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