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Human CASP8(Caspase 8) ELISA Kit

Citations(10)Uniprot : Q14790

Cat.No.:E-EL-H0659
Reactivity: Human

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human CASP8 in samples. No significant cross-reactivity or interference between Human CASP8 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human CASP8 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CASP8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CASP8. You can calculate the concentration of Human CASP8 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CASP8 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CASP8 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.55	1.21	4.89	0.53	1.13	4.48
Standard deviation	0.03	0.06	0.16	0.04	0.05	0.14
CV (%)	5.45	4.96	3.27	7.55	4.42	3.13

Recovery

The recovery of Human CASP8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-108	99
EDTA plasma (n=8)	90-104	95
Cell culture media (n=8)	88-104	95

Linearity

Samples were spiked with high concentrations of Human CASP8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q14790
Research Area	Cancer, Cell Biology, Metabolism

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

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Citations

Pharmaceutics (2021) IF: 6.321
Comparative Study of the Pharmacological Properties and Biological Effects of Polygonum aviculare L. herba Extract-Entrapped Liposomes versus Quercetin-Entrapped Liposomes on Doxorubicin-Induced Toxicity on HUVECs

DOI: 10.3390/pharmaceutics13091418

PMID: 34575493
Sample: HUVEC
LIFE SCIENCES (2022) IF: 5.037
Integration of synthetic and natural derivatives revives the therapeutic potential of temozolomide against glioma- an in vitro and in vivo perspective

DOI: 10.1016/j.lfs.2022.120609

PMID: 35526592
Sample: C6 cell,U-87 MG cell,LN229 cell
Pharmaceuticals (2022) IF: 5.215
Synthesis and Chemopreventive Potential of 5-FU/Genistein Hybrids on Colorectal Cancer Cells

DOI: 10.3390/ph15101299

PMID: 36297411
Sample: SW480 cells,SW620 cells
Anaesthesia Critical Care & Pain Medicine (2021) IF: 4.132
Mortality of spontaneous intracerebral haemorrhage patients and high serum caspase-8 concentrations

DOI: 10.1016/j.accpm.2021.100969

PMID: 34718185
Sample: serum
NEUROLOGICAL SCIENCES (2021) IF: 3.307
Low blood caspase-8 levels in survivor patients of traumatic brain injury

DOI: 10.1007/s10072-021-05205-2

PMID: 33759054
Sample: Serum
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL (2020) IF: 2.363
Evaluation of the Effects of Nobiletin on Toll-Like Receptor 3 Signaling Pathways in Prostate Cancer In Vitro

DOI: 10.1080/01635581.2020.1841247

Sample: Cell culture supernatant
Medicina Intensiva (2020) IF: 2.363
Blood caspase-8 concentrations and mortality among septic patients

DOI: 10.1016/j.medin.2020.06.016

Sample: serum
Medicina Intensiva (2021) IF: 2.491
Association between blood caspase-8 levels and mortality of patients with malignant middle cerebral artery infarction

DOI: 10.1016/j.medin.2021.03.001

PMID: 33926751
Sample: Serum
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE (2022) IF: 2.803
Mortality prediction of septic patients by blood caspase-8 levels

DOI: 10.1016/j.diagmicrobio.2022.115639

PMID: 35180643
Sample: serum
SCIENTIA PHARMACEUTICA (2022)
Colorectal Cancer Chemoprevention by S-Allyl Cysteine–Caffeic Acid Hybrids: In Vitro Biological Activity and In Silico Studies

DOI: 10.3390/scipharm90030040

Sample: SW480 cell

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