Human COL5α2 (Collagen Type V Alpha 2) CLIA Kit (E-CL-H0640)

Name: Human COL5α2 (Collagen Type V Alpha 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0640
Price: 180.0 USD
Availability: InStock

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Human COL5α2 (Collagen Type V Alpha 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.94 ng/mL
Detection Range	1.56-100 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human COL5α2 in samples.No significant cross-reactivity or interference between Human COL5α2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
100	36374.000	39268.000	37821.000	37790
50	15151.000	17629.000	16390.000	16359
25	8142.000	7628.000	7885.000	7854
12.5	3949.000	4043.000	3996.000	3965
6.25	2077.000	1979.000	2028.000	1997
3.13	1021.000	1003.000	1012.000	981
1.56	567.000	599.000	583.000	552
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	5.550	15.160	45.910	5.300	15.650	47.400
Standard deviation	0.670	1.420	3.270	0.610	1.610	4.350
CV（%）	12.070	9.370	7.120	11.510	10.290	9.180

Recovery

The recovery of Human COL5α2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	84-96	90
EDTA plasma （n=8）	94-111	102
Cell culture media （n=8）	93-109	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human COL5α2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-111	94-106	94-106
1:2	Average	102	100	99
1:4	Range	91-102	88-103	90-105
1:4	Average	97	94	98
1:8	Range	91-104	90-105	95-111
1:8	Average	97	96	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL5α2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL5α2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL5α2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human COL5α2. You can calculate the concentration of Human COL5α2 in the samples by comparing the RLU value of the samples to the standard curve.

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