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Human CRP(C-Reactive Protein) ELISA Kit

  • Cat.No.:E-EL-H0043

  • Reactivity: Human

To Purchase E-EL-H0043

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.39-25 ng/mL
Sensitivity 0.23 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human CRP in samples. No significant cross-reactivity or interference between Human CRP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human CRP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CRP. You can calculate the concentration of Human CRP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CRP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CRP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 2.50 4.90 16.80 2.50 4.70 18.20
Standard deviation 0.10 0.20 0.60 0.20 0.30 0.70
CV (%) 4.00 4.08 3.57 8.00 6.38 3.85


The recovery of Human CRP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-102 95
EDTA plasma (n=8) 85-97 90
Cell culture media (n=8) 88-103 94


Samples were spiked with high concentrations of Human CRP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P02741
SynonymsPTX1, Pentraxin 1
Research AreaCancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


  1. Biomedicines (2021) IF: 6.081
    Post-COVID-19 Patients Who Develop Lung Fibrotic-like Changes Have Lower Circulating Levels of IFN-β but Higher Levels of IL-1α and TGF-β

    DOI: 10.3390/biomedicines9121931

    Sample: plasma
    Differential levels of inflammatory and neuroendocrine markers in the hippocampus and anterior cingulate cortex of bipolar disorder subjects: A post-mortem study

    DOI: 10.1016/j.bbi.2020.08.034

    PMID: 32949690

    Sample: Tissue homogenate
  3. Nutrients (2022) IF: 6.706
    Relationship between the Dietary Inflammatory Index Score and Cytokine Levels in Chinese Pregnant Women during the Second and Third Trimesters

    DOI: 10.3390/nu15010194

    Sample: serum
  4. ANALYTICA CHIMICA ACTA (2022) IF: 6.911
    A plasmonic ELISA for multi-colorimetric sensing of C-reactive protein by using shell dependent etching of Ag coated Au nanobipyramids

    DOI: 10.1016/j.aca.2022.340129

    PMID: 35934405

    Sample: AuNBP@Ag
    Clinical, inflammatory and microbiological outcomes of full‐month scaling with adjunctive glycine powder air‐polishing. A randomized trial

    DOI: 10.1111/jcpe.13400

    Sample: Serum,Gingival Crevicular Fluid
    Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis

    DOI: 10.3748/wjg.v27.i38.6489

    PMID: 34720537

    Sample: serum
    Effect of fructooligosaccharide on endothelial function in CKD patients: a randomized controlled trial

    DOI: 10.1093/ndt/gfaa335

    Sample: serum
  8. NUTRITION (2021) IF: 4.008
    Changes in plasma fatty acids and related biomarkers during transition to an exclusively plant- and fish-based diet in healthy adults

    DOI: 10.1016/j.nut.2021.111306

    PMID: 34166896

    Sample: Plasma
  9. Scientific Reports (2021) IF: 4.38
    Obesity-related proteins score as a potential marker of breast cancer risk

    DOI: 10.1038/s41598-021-87583-3

    PMID: 33859244

    Sample: Plasma
  10. Nutrients (2020) IF: 4.546
    Intermittent Fasting Enhanced the Cognitive Function in Older Adults with Mild Cognitive Impairment by Inducing Biochemical and Metabolic changes: A 3-Year Progressive Study

    DOI: 10.3390/nu12092644

    PMID: 32872655

    Sample: Plasma
    Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression

    DOI: 10.1016/j.jpsychires.2022.04.002

    PMID: 35429740

    Sample: serum
  12. IUBMB LIFE (2015) IF: 3.143
    TSG attenuates LPC‐induced endothelial cells inflammatory damage through notch signaling inhibition

    DOI: 10.1002/iub.1458

    Sample: Cell culture supernatant
    Faecal but not serum calprotectin levels look promising in predicting active disease in Behçet's syndrome patients with gastrointestinal involvement.

    PMID: 30582504

    Sample: stool
  14. Kidney Diseases (2018) IF: 3.222
    Prognostic Significance of Serum NGAL and Troponin I against Acute Kidney Injury in Egyptian ICU Patients after Open Heart Surgery: A Pilot Study

    DOI: 10.1159/000490246

    Sample: Serum
  15. AUTOIMMUNITY (2020) IF: 2.125
    Auto-reactivity against gut bacterial peptides in patients with late-onset diabetes

    DOI: 10.1080/08916934.2020.1818232

    PMID: 32924613

    Sample: Serum
  16. BMC Neurology (2022) IF: 2.474
    Changes of biochemical biomarkers in the serum of children with convulsion status epilepticus: a prospective study

    DOI: 10.1186/s12883-022-02686-2

    PMID: 35624413

    Sample: serum
  17. Annals of Palliative Medicine (2021) IF: 2.595
    The effects of blood purification combined with antibiotics on extravascular lung water index, inflammatory factors, and prognosis of patients with severe acute pancreatitis complicated with acute respiratory distress syndrome.

    DOI: 10.21037/apm-21-2168

    Sample: blood
  18. PLoS One (2017) IF: 2.806
    High anti-human cytomegalovirus antibody levels are associated with the progression of essential hypertension and target organ damage in Han Chinese population

    DOI: 10.1371/journal.pone.0181440

    Sample: Serum
    Sex difference in IL-6 modulation of cognition among Chinese individuals with major depressive disorder

    DOI: 10.1016/j.jocn.2019.10.002

    PMID: 31629608

    Sample: Serum
  20. BMC Anesthesiology (2019) IF: 1.619
    A comparison of effects of scalp nerve block and local anesthetic infiltration on inflammatory response, hemodynamic response, and postoperative pain in patients undergoing craniotomy for cerebral aneurysms: a randomized controlled trial

    DOI: 10.1186/s12871-019-0760-4

    Sample: plasm
  21. Journal of Stroke & Cerebrovascular Diseases (2020) IF: 1.787
    Incremental Accuracy of Blood Biomarkers for Predicting Clinical Outcomes After Intracerebral Hemorrhage

    DOI: 10.1016/j.jstrokecerebrovasdis.2020.105537

    PMID: 33338706

    Sample: serum
  22. European Journal of Obstetrics & Gynecology and Reproductive Biology (2020) IF: 1.868
    A comparative study of autologous rectus fascia pubovaginal sling surgery and synthetic transobturator vaginal tape procedure in treatment of women with urodynamic stress urinary incontinence

    DOI: 10.1016/j.ejogrb.2020.06.062

    Sample: blood
  23. Sport Sciences for Health (2022)
    The effects of aerobic exercise and sertraline on pro-inflammatory indices and amelioration of neurotrophic factors in patients who have undergone CABG

    DOI: 10.1007/s11332-022-00992-3

    Sample: plasma
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  • Q&A

H****ESubmitted [ Mar 07 2023 ]

Asked: What is the concentration of crp that one can expect to calculate for human SALIVA using the Elabscience ELISA (E-EL-H0043)?



adminSubmitted [ Mar 07 2023 ]

Answered: For the kit E-EL-H0043, the tested human saliva samples were about 20-125ng/mL, it is recommended to dilute at 1-10 fold.


h***** Submitted [ Mar 07 2023 ]

Asked: What is the concentration of crp that one can expect to calculate for human serum using the Elabscience ELISA (E-EL-H0043)?



adminSubmitted [ Mar 07 2023 ]

Answered: We have tested normal human serum samples with E-EL-H0043 kit, the reference values were about 4.7-17ug/mL, it is recommended to dilute at 500-5000 fold.

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