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Human GHRL(Ghrelin) ELISA Kit

  • Cat.No.:E-EL-H1919

  • Reactivity: Human

To Purchase E-EL-H1919

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes GHRL in samples. No significant cross-reactivity or interference between GHRL and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of GHRL concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GHRL. During the reaction, GHRL in the sample or standard competes with a fixed amount of GHRL on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GHRL. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GHRL in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level GHRL were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level GHRL were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 0.90 4.10 0.50 0.90 3.70
Standard deviation 0.03 0.04 0.13 0.03 0.04 0.13
CV (%) 6.00 4.44 3.17 6.00 4.44 3.51


The recovery of GHRL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-97 92
EDTA plasma(n=8) 95-108 101
Cell culture media(n=8) 91-105 96


Samples were spiked with high concentrations of Human GHRL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: Q9UBU3
SynonymsGHRL, MTLRP, Ghrelin
Research AreaCancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


  1. Frontiers in Nutrition (2022) IF: 6.59
    Sleep duration of lactating mothers and its relationship with feeding pattern, milk macronutrients and related serum factors: A combined longitudinal cohort and cross-sectional study

    DOI: 10.3389/fnut.2022.973291

    PMID: 36110402

    Sample: plasma
    Effectiveness of a polyphenolic extract (Lippia citriodora and Hibiscus sabdariffa) on appetite regulation in overweight and obese grade I population: an 8-week randomized, double-blind, cross-over, placebo-controlled trial

    DOI: 10.1007/s00394-021-02678-x

    PMID: 34591168

    Sample: blood
    Associations between serum ghrelin and knee symptoms, joint structures and cartilage or bone biomarkers in patients with knee osteoarthritis

    DOI: 10.1016/j.joca.2017.05.015

    Sample: Serum
  4. NEUROPEPTIDES (2021) IF: 3.286
    Changes in ghrelin, substance P and vasoactive intestinal peptide levels in the gastroduodenal mucosa of patients with morbid obesity

    DOI: 10.1016/j.npep.2021.102164

    PMID: 34146741

    Sample: serum
  5. International Journal of Environmental Research and Public Health (2021) IF: 3.39
    A Case Control Study on Serum Levels of Potential Biomarkers in Male Breast Cancer Patients

    DOI: 10.3390/ijerph18094852

    PMID: 34062830

    Sample: Serum
  6. CYTOKINE (2021) IF: 3.861
    The effect of umbilical cord blood spexin, free 25(OH) vitamin D3 and adipocytokine levels on intrauterine growth and anthropometric measurements in newborns

    DOI: 10.1016/j.cyto.2021.155578

    PMID: 34010726

    Sample: Serum
  7. APPETITE (2021) IF: 3.868
    Acute and short-term effects of Lactobacillus paracasei subsp. paracasei 431 and inulin intake on appetite control and dietary intake: A two-phases randomized, double blind, placebo-controlled study

    DOI: 10.1016/j.appet.2021.105855

    PMID: 34915104

    Sample: serum
  8. International Journal of Environmental Research and Public Health (2019) IF: 2.468
    Effect of Sequence of Fruit Intake in a Meal on Satiety

    DOI: 10.3390/ijerph16224464

    PMID: 31766283

    Sample: Plasma
  9. PSYCHIATRY RESEARCH (2017) IF: 2.528
    The association of serum nesfatin-1 and ghrelin levels with metabolic syndrome in patients with schizophrenia

    DOI: 10.1016/j.psychres.2017.12.041

    Sample: Serum
  10. Biomed Research International (2018) IF: 2.583
    High Ghrelin Level Predicts the Curve Progression of Adolescent Idiopathic Scoliosis Girls

    DOI: 10.1155/2018/9784083

    Sample: Serum
    Serum nesfatin-1, ghrelin, and lipid levels in adolescents with first episode drug naïve unipolar depression

    DOI: 10.1080/08039488.2020.1772363

    Sample: serum
  12. Journal of Taibah University Medical Sciences (2021)
    Does kisspeptin act as a neuropeptide or as an adipokine in obese people?

    DOI: 10.1016/j.jtumed.2021.07.010

    Sample: serum
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