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Human GROβ/CXCL2(Growth Regulated Oncogene Beta) ELISA Kit

  • Cat.No.:E-EL-H1904

  • Reactivity: Human

To Purchase E-EL-H1904

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human GROβ/CXCL2 in samples. No significant cross-reactivity or interference between Human GROβ/CXCL2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human GROβ/CXCL2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GROβ/CXCL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GROβ/CXCL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GROβ/CXCL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GROβ/CXCL2. You can calculate the concentration of Human GROβ/CXCL2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human GROβ/CXCL2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human GROβ/CXCL2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 54.70 135.50 382.40 59.70 146.50 360.80
Standard deviation 3.70 7.60 15.30 3.00 6.60 17.70
CV (%) 6.76 5.61 4.00 5.03 4.51 4.91


The recovery of Human GROβ/CXCL2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-106 98
EDTA plasma (n=8) 91-104 97
Cell culture media (n=8) 86-97 91


Samples were spiked with high concentrations of Human GROβ/CXCL2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P19875
SynonymsCXCL2, MIP-2a, GRO2, SCYB2, GROb, MGSA-B, CINC2a
Research AreaImmunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Nature Communications (2022) IF: 17.694
    ARID1A loss induces polymorphonuclear myeloid-derived suppressor cell chemotaxis and promotes prostate cancer progression

    DOI: 10.1038/s41467-022-34871-9

    PMID: 36435834

    Sample: serum,prostate tissue
    LncRNA MEG3 ameliorates NiO nanoparticles-induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway

    DOI: 10.1002/tox.23464

    PMID: 35006638

    Sample: A549 cell
  3. Journal of the International Society of Sports Nutrition (2019) IF: 3.841
    Effects of a polysaccharide-based multi-ingredient supplement on salivary immunity in non-elite marathon runners

    DOI: 10.1186/s12970-019-0281-z

    Sample: saliva
    Changes in the BTK/NF-κB signaling pathway and related cytokines in different stages of neuromyelitis optica spectrum disorders

    DOI: 10.1186/s40001-022-00723-x

    PMID: 35729649

    Sample: blood
  5. PLoS One (2018) IF: 2.766
    Salivary immunity and lower respiratory tract infections in non-elite marathon runners

    DOI: 10.1371/journal.pone.0206059

    PMID: 30462646

    Sample: saliva
    Role of the long non-coding RNA, SPRR2C, based on an in vitro psoriatic keratinocyte cell model

    DOI: 10.1684/ejd.2022.4247

    PMID: 35866915

    Sample: Ker-CT cell,HaCaT cell
    Cytokine–chemokine and cognitive profile of multiple sclerosis patients with predominant optic nerve and spinal cord involvement

    DOI: 10.1080/10790268.2019.1666238

    Sample: serum
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