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Human HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

  • Cat.No.:E-EL-H6066

  • Reactivity: Human

To Purchase E-EL-H6066

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 62.50-4000 pg/mL
Sensitivity 37.50 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human HIF-1α in samples. No significant cross-reactivity or interference between Human HIF-1α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human HIF-1α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HIF-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HIF-1α. You can calculate the concentration of Human HIF-1α in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human HIF-1α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human HIF-1α were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 177.29 422.96 1738.08 194.33 486.58 1980.59
Standard deviation 9.41 20.26 71.09 10.47 24.09 91.11
CV (%) 5.31 4.79 4.09 5.39 4.95 4.60


The recovery of Human HIF-1α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-109 102
EDTA plasma (n=8) 99-109 104
Cell culture media (n=8) 101-112 105


Samples were spiked with high concentrations of Human HIF-1α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q16665
SynonymsHIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor
Research AreaCell biology,Epigenetics and Nuclear Signaling

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Asian Journal of Pharmaceutical Sciences (2020) IF: 3.968
    Enhanced lysosome escape mediated by 1,2-dicarboxylic-cyclohexene anhydride-modified poly-l-lysine dendrimer as a gene delivery system

    DOI: 10.1016/j.ajps.2019.12.001

    PMID: 99999999

    Sample: Cell lysate
  2. Journal of Cellular Biochemistry (2017) IF: 3.085
    Shielding Engineered Islets With Mesenchymal Stem Cells Enhance Survival Under Hypoxia.

    DOI: 10.1002/jcb.25885

    PMID: 28098405

    Sample: Cell lysate
  3. International Journal of Molecular Medicine (2018) IF: 2.784
    Role of TXNDC5 in tumorigenesis of colorectal cancer cells: In vivo and in vitro evidence.

    DOI: 10.3892/ijmm.2018.3664

    PMID: 29749460

    Sample: Tissue homogenate
  4. Life Sciences (2017) IF: 2.936
    Hypoxia Primed Placental Mesenchymal Stem Cells For Wound Healing.

    DOI: 10.1016/j.lfs.2017.06.016

    PMID: 28625360

    Sample: Cell lysate
  5. Plos One (2016) IF: 2.806
    Involvement Of Renin-Angiotensin System In Retinopathy Of Prematurity - A Possible Target For Therapeutic Intervention.

    DOI: 10.1371/journal.pone.0168809

    PMID: 28033392

    Sample: Biological fluid
  6. European Journal of Pharmacology (2016) IF: 2.896
    Small Molecules Exert Anti-Apoptotic Effect And Reduce Oxidative Stressaugmenting Insulin Secretion In Stem Cells Engineered Isletsagainst Hypoxia.

    DOI: 10.1016/j.ejphar.2016.09.012

    PMID: 27614129

    Sample: Cell culture supernatant
  7. Springerplus (2016) IF: 1.13
    Hypoxia-Induced Inflammation And Purinergic Signaling In Cross Clamping The Human Aorta.

    DOI: 10.1186/s40064-015-1651-x

    PMID: 26759741

    Sample: Serum
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