Current Position:Home>>Kits>>ELISA Kits>> Human HMGB-1(High Mobility Group Protein B1) ELISA Kit
Cat.No.:E-EL-H1554
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 31.25-2000 pg/mL |
Sensitivity | 18.75 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human HMGB-1 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human HMGB-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human HMGB-1 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 99.32 | 202.75 | 693.26 | 108.96 | 208.03 | 746.59 |
Standard deviation | 5.84 | 8.62 | 35.56 | 5.86 | 9.76 | 26.06 |
CV (%) | 5.88 | 4.25 | 5.13 | 5.38 | 4.69 | 3.49 |
The recovery of Human HMGB-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 94-108 | 100 |
EDTA plasma (n=8) | 94-107 | 100 |
Cell culture media (n=8) | 93-105 | 99 |
Samples were spiked with high concentrations of Human HMGB-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P09429
|
Synonyms | HMGB1, HMG1, HMG3, SBP-1, Amphoterin |
Research Area | Cancer, Epigenetics and Nuclear Signaling |
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1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
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Sample: 4T1 cells |
Sample: A549 cell,Lewis cell |
Sample: serum |
PMID: 35768823 |
Sample: cerebrospinal fluid (CSF),peripheral monocytes |
DOI: 10.1111/liv.15457 |
|
PMID: 36251580 |
Sample: serum |
Sample: Cell culture medium |
PMID: 34384355 |
Sample: serum |
Sample: Serum |
Sample: Cell culture supernatant |
Sample: CAL-27 cell |
Sample: plasma |
PMID: 35551614 |
Sample: A549 cell |
PMID: 34933679 |
Sample: Jurkat cell,HL-60 cell |
PMID: 31934300 |
Sample: serum,HUVECs |
DOI: 10.2147/DDDT.S325814 |
|
PMID: 34764638 |
Sample: Nthy-ori 3–1 cells |
DOI: 10.3390/cells9122585 |
|
PMID: 33287126 |
Sample: Cell culture supernatant |
Sample: Cell culture supernatant |
DOI: 10.1111/adb.12543 |
|
Sample: Plasma |
DOI: 10.1111/jcmm.14789 |
|
Sample: Serum,Cell culture supernatant |
PMID: 30676239 |
Sample: Cell culture medium |
Sample: supernatant |
Sample: Serum |
Sample: chondrocytes |
PMID: 30940475 |
Sample: Cell culture medium |
Sample: Plasma |
Sample: serum |
DOI: 10.1155/2017/3605350 |
|
Sample: Serum |
PMID: 34276038 |
Sample: serum |
Sample: Cell culture supernatant |
DOI: 10.3390/life12030432 |
|
Sample: Plasma |
Sample: gastric cancer (GC) cells |
PMID: 34727314 |
Sample: serum |
DOI: 10.7717/peerj.8514 |
|
Sample: Cell culture supernatant |
PMID: 31158358 |
Sample: Serum |
Sample: Serum |
Sample: Cell culture supernatant |
Sample: Serum,Cell culture supernatant |
PMID: 32965599 |
Sample: Serum |
PMID: 30562142 |
Sample: Plasma |
Sample: Serum,Urine |
Sample: serum |
Sample: Cell culture supernatant |
PMID: 30956264 |
Sample: Cell culture supernatant |
Sample: serum |