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Human HMGB-1(High Mobility Group Protein B1) ELISA Kit

Citations(44)Uniprot : P09429

Cat.No.:E-EL-H1554
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human HMGB-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human HMGB-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human HMGB-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	99.32	202.75	693.26	108.96	208.03	746.59
Standard deviation	5.84	8.62	35.56	5.86	9.76	26.06
CV (%)	5.88	4.25	5.13	5.38	4.69	3.49

Recovery

The recovery of Human HMGB-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-108	100
EDTA plasma (n=8)	94-107	100
Cell culture media (n=8)	93-105	99

Linearity

Samples were spiked with high concentrations of Human HMGB-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P09429
Synonyms	HMGB1, HMG1, HMG3, SBP-1, Amphoterin
Research Area	Cancer, Epigenetics and Nuclear Signaling

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

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Acta Pharmaceutica Sinica B (2022) IF: 14.903

Reversing the PAI-1-induced fibrotic immune exclusion of solid tumor by multivalent CXCR4 antagonistic nano-permeator

DOI: 10.1016/j.apsb.2022.12.012
	Sample: 4T1 cells

JOURNAL OF NANOBIOTECHNOLOGY (2021) IF: 10.435

Synergy of nanodiamond–doxorubicin conjugates and PD-L1 blockade effectively turns tumor-associated macrophages against tumor cells

DOI: 10.1186/s12951-021-01017-w
	Sample: A549 cell,Lewis cell

SCHIZOPHRENIA BULLETIN (2021) IF: 9.3059

High Mobility Group Protein 1 and Dickkopf-Related Protein 1 in Schizophrenia and Treatment-Resistant Schizophrenia: Associations With Interleukin-6, Symptom Domains, and Neurocognitive Impairments

DOI: 10.1093/schbul/sbaa136
	Sample: serum

Journal of Neuroinflammation (2022) IF: 9.587

Transient post-operative overexpression of CXCR2 on monocytes of traumatic brain injury patients drives monocyte chemotaxis toward cerebrospinal fluid and enhances monocyte-mediated immunogenic cell death of neurons in vitro

DOI: 10.1186/s12974-022-02535-6
PMID: 35768823	Sample: cerebrospinal fluid (CSF),peripheral monocytes

LIVER INTERNATIONAL (2022) IF: 8.754

Human poliovirus receptor contributes to biliary atresia pathogenesis by exacerbating natural-killer-cell-mediated bile duct injury

DOI: 10.1111/liv.15457
PMID: 36251580	Sample: serum

BIOMATERIALS (2018) IF: 8.8059

Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma

DOI: 10.1016/j.biomaterials.2018.07.035
	Sample: Cell culture medium

JOURNAL OF HEADACHE AND PAIN (2021) IF: 7.277

HMGB1, NLRP3, IL-6 and ACE2 levels are elevated in COVID-19 with headache: a window to the infection-related headache mechanism

DOI: 10.1186/s10194-021-01306-7
PMID: 34384355	Sample: serum

Frontiers in Immunology (2018) IF: 7.561

Profiling of Canonical and Non-Traditional Cytokine Levels in Interferon-β-Treated Relapsing–Remitting-Multiple Sclerosis Patients

DOI: 10.3389/fimmu.2018.01240
	Sample: Serum

JOURNAL OF CONTROLLED RELEASE (2017) IF: 7.786

Harnessing the cross-talk between tumor cells and tumor-associated macrophages with a nano-drug for modulation of glioblastoma immune microenvironment

DOI: 10.1016/j.jconrel.2017.10.024
	Sample: Cell culture supernatant

Frontiers in Oncology (2021) IF: 6.244

CD68+ Macrophage Infiltration Associates With Poor Outcome of HPV Negative Oral Squamous Carcinoma Patients Receiving Radiation: Poly(I:C) Enhances Radiosensitivity of CAL-27 Cells but Promotes Macrophage Recruitment Through HMGB1

DOI: 10.3389/fonc.2021.740622
	Sample: CAL-27 cell

ASN Neuro (2022) IF: 5.2

Translocation of High Mobility Group Box 1 From the Nucleus to the Cytoplasm in Depressed Patients With Epilepsy

DOI: 10.1177/17590914221136662
	Sample: plasma

Cell Communication and Signaling (2022) IF: 5.712

The ROCK-ezrin signaling pathway mediates LPS-induced cytokine production in pulmonary alveolar epithelial cells

DOI: 10.1186/s12964-022-00879-3
PMID: 35551614	Sample: A549 cell

Cancer Cell International (2021) IF: 5.722

Extracellular HMGB1 interacts with RAGE and promotes chemoresistance in acute leukemia cells

DOI: 10.1186/s12935-021-02387-9
PMID: 34933679	Sample: Jurkat cell,HL-60 cell

American Journal of Translational Research (2019) IF: 4.06

HMGB1-induced endothelial cell pyroptosis is involved in systemic inflammatory response syndrome following radiofrequency ablation of hepatic hemangiomas


PMID: 31934300	Sample: serum,HUVECs

Drug Design Development and Therapy (2021) IF: 4.162

Prunella vulgaris L. Attenuates Experimental Autoimmune Thyroiditis by Inhibiting HMGB1/TLR9 Signaling

DOI: 10.2147/DDDT.S325814
PMID: 34764638	Sample: Nthy-ori 3–1 cells

Cells (2020) IF: 4.366

Role of HMGB1/TLR4 Axis in Ischemia/Reperfusion-Impaired Extracellular Glutamate Clearance in Primary Astrocytes

DOI: 10.3390/cells9122585
PMID: 33287126	Sample: Cell culture supernatant

BIOCHEMICAL PHARMACOLOGY (2017) IF: 4.581

Synthetic anti-endotoxin peptides inhibit cytoplasmic LPS-mediated responses

DOI: 10.1016/j.bcp.2017.05.015
	Sample: Cell culture supernatant

ADDICTION BIOLOGY (2017) IF: 4.603

Young alcohol binge drinkers have elevated blood endotoxin, peripheral inflammation and low cortisol levels: neuropsychological correlations in women

DOI: 10.1111/adb.12543
	Sample: Plasma

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2019) IF: 4.658

Cigarette smoke‐induced HMGB1 translocation and release contribute to migration and NF‐κB activation through inducing autophagy in lung macrophages

DOI: 10.1111/jcmm.14789
	Sample: Serum,Cell culture supernatant

Nanomedicine (2019) IF: 4.717

Doxorubicin conjugated with nanodiamonds and in free form commit glioblastoma cells to heterodromous fates

DOI: 10.2217/nnm-2018-0330
PMID: 30676239	Sample: Cell culture medium

Nanomedicine (2017) IF: 4.727

Monocyte-mediated chemotherapy drug delivery in glioblastoma

DOI: 10.2217/nnm-2017-0266
	Sample: supernatant

JOURNAL OF AFFECTIVE DISORDERS (2021) IF: 4.839

In schizophrenia, immune-inflammatory pathways are strongly associated with depressive and anxiety symptoms, which are part of a latent trait which comprises neurocognitive impairments and schizophrenia symptoms.

DOI: 10.1016/j.jad.2021.03.062
	Sample: Serum

BMB Reports (2018) IF: 3.085

29-kDa FN-f inhibited autophagy through modulating localization of HMGB1 in human articular chondrocytes

DOI: 10.5483/BMBRep.2018.51.10.058
	Sample: chondrocytes

BRAIN RESEARCH BULLETIN (2019) IF: 3.103

Role of toll-like receptor 4 in the regulation of the cell death pathway and neuroinflammation

DOI: 10.1016/j.brainresbull.2019.03.015
PMID: 30940475	Sample: Cell culture medium

JOURNAL OF NEUROIMMUNOLOGY (2020) IF: 3.125

The association between plasma HMGB1 and sRAGE and clinical outcome in intracerebral hemorrhage

DOI: 10.1016/j.jneuroim.2020.577266
	Sample: Plasma

Frontiers in Physiology (2019) IF: 3.201

Evidence for SIRT1 Mediated HMGB1 Release From Kidney Cells in the Early Stages of Hemorrhagic Shock

DOI: 10.3389/fphys.2019.00854
	Sample: serum

MEDIATORS OF INFLAMMATION (2017) IF: 3.232

Short-Term Postoperative Cognitive Dysfunction and Inflammatory Response in Patients Undergoing Cytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy: A Pilot Study

DOI: 10.1155/2017/3605350
	Sample: Serum

SHOCK (2021) IF: 3.454

The Value of Extracellular Cold-Inducible RNA-Binding Protein (eCIRP) in Predicting the Severity and Prognosis of Patients After Cardiac Arrest: A Preliminary Observational Study

DOI: 10.1097/SHK.0000000000001702
PMID: 34276038	Sample: serum

TOXICOLOGY (2015) IF: 3.621

Ethanol directly induced HMGB1 release through NOX2/NLRP1 inflammasome in neuronal cells

DOI: 10.1016/j.tox.2015.06.006
	Sample: Cell culture supernatant

Life-Basel (2022) IF: 3.817

Response of Circulating Inflammatory Markers to Intermittent Hypoxia-Hyperoxia Training in Healthy Elderly People and Patients with Mild Cognitive Impairment

DOI: 10.3390/life12030432
	Sample: Plasma

BIOCHEMICAL GENETICS (2022) IF: 2.22

Isoquercitrin Induces Endoplasmic Reticulum Stress and Immunogenic Cell Death in Gastric Cancer Cells

DOI: 10.1007/s10528-022-10309-1
	Sample: gastric cancer (GC) cells

INTERNATIONAL UROLOGY AND NEPHROLOGY (2021) IF: 2.37

High mobility group box 1 and homocysteine as preprocedural predictors for contrast-induced acute kidney injury after percutaneous coronary artery intervention

DOI: 10.1007/s11255-021-03050-y
PMID: 34727314	Sample: serum

PeerJ (2020) IF: 2.379

Glycyrrhizin suppresses epithelial-mesenchymal transition by inhibiting high-mobility group box1 via the TGF-β1/Smad2/3 pathway in lung epithelial cells

DOI: 10.7717/peerj.8514
	Sample: Cell culture supernatant

CLINICAL BIOCHEMISTRY (2019) IF: 2.43

HMGB1 and SEPP1 as predictors of hepatocellular carcinoma in patients with viral C hepatitis: Effect of DAAs

DOI: 10.1016/j.clinbiochem.2019.05.017
PMID: 31158358	Sample: Serum

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2017) IF: 2.466

Relationships of BRAF mutation and HMGB1 to papillary thyroid carcinoma

DOI: 10.1016/j.bbrc.2017.03.117
	Sample: Serum

Thoracic Cancer (2018) IF: 2.569

Recombined humanized endostatin‐induced suppression of HMGB1 expression inhibits proliferation of NSCLC cancer cells

DOI: 10.1111/1759-7714.12905
	Sample: Cell culture supernatant

DNA AND CELL BIOLOGY (2016) IF: 2.574

HMGB1 Enhances Drug Resistance and Promotes In Vivo Tumor Growth of Lung Cancer Cells

DOI: 10.1089/dna.2016.3360
	Sample: Serum,Cell culture supernatant

METABOLIC BRAIN DISEASE (2020) IF: 2.726

Chronic fatigue syndrome and fibromyalgia-like symptoms are an integral component of the phenome of schizophrenia: neuro-immune and opioid system correlates

DOI: 10.1007/s11011-020-00619-x
PMID: 32965599	Sample: Serum

Genetic Testing and Molecular Biomarkers (2019) IF: 1.121

Association of High Mobility Group Box Protein B1 Gene Polymorphisms with Pneumonia Susceptibility and Severity

DOI: 10.1089/gtmb.2018.0174
PMID: 30562142	Sample: Plasma

Experimental and Therapeutic Medicine (2017) IF: 1.261

Blood purification treatment initiated at the time of sepsis diagnosis effectively attenuates serum HMGB1 upregulation and improves patient prognosis

DOI: 10.3892/etm.2017.4854
	Sample: Serum,Urine

EPILEPTIC DISORDERS (2020) IF: 1.276

HMGB‐1, TLR4, IL‐1R1, TNF‐α, and IL‐1β: novel epilepsy markers?

DOI: 10.1684/epd.2020.1155
	Sample: serum

Experimental and Therapeutic Medicine (2018) IF: 1.41

Necrosis of osteosarcoma cells induces the production and release of high‑mobility group box 1 protein

DOI: 10.3892/etm.2017.5415
	Sample: Cell culture supernatant

BIOLOGICAL & PHARMACEUTICAL BULLETIN (2019) IF: 1.54

Triptolide suppresses growth of breast cancer by targeting HMGB1 in vitro and in vivo

DOI: 10.1248/bpb.b18-00818
PMID: 30956264	Sample: Cell culture supernatant

Indian Journal of Hematology and Blood Transfusion (2022) IF: 0.915

Effects of AGEs, sRAGE and HMGB1 on Clinical Outcomes in Multiple MyelomaIn the PDF, in Header of all pages, Journal title should be abbreviated as "Indian J Hematol Blood Transfus".

DOI: 10.1007/s12288-022-01574-6
	Sample: serum

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