Click here to view cell apoptosis assay products

Human HSP-70/HSPA9(Heat Shock 70 kDa Protein 9) ELISA Kit

  • Cat.No.:E-EL-H1863

  • Reactivity: Human

To Purchase E-EL-H1863

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.78-50 ng/mL
Sensitivity 0.47 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human HSP-70/HSPA9 in samples. No significant cross-reactivity or interference between Human HSP-70/HSPA9 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human HSP-70/HSPA9 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-70/HSPA9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-70/HSPA9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-70/HSPA9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HSP-70/HSPA9. You can calculate the concentration of Human HSP-70/HSPA9 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human HSP-70/HSPA9 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human HSP-70/HSPA9 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 2.30 6.00 22.60 2.30 5.60 24.10
Standard deviation 0.10 0.30 1.10 0.10 0.30 0.90
CV (%) 4.35 5.00 4.87 4.35 5.36 3.73


The recovery of Human HSP-70/HSPA9 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-108 100
EDTA plasma (n=8) 91-106 97
Cell culture media (n=8) 91-103 98


Samples were spiked with high concentrations of Human HSP-70/HSPA9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P38646
SynonymsHSPA9, CSA, GRP-75, GRP75, HSPA9B, MOT, MOT2, MTHSP75, PBP74
Research AreaCancer, Metabolism, Neuroscience, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

    A novel electrochemical immunosensor based on PG for early screening of depression markers-heat shock protein 70

    DOI: 10.1016/j.bios.2018.03.049

    Sample: Plasma
  2. CANCER LETTERS (2020) IF: 7.36
    Small extracellular vesicle-bound vascular endothelial growth factor secreted by carcinoma-associated fibroblasts promotes angiogenesis in a bevacizumab-resistant manner

    DOI: 10.1016/j.canlet.2020.08.030

    PMID: 32860852

    Sample: Cell culture supernatant
  3. Materials Science & Engineering C-Materials for Biological Applications (2020) IF: 5.88
    Development of a sensitive electrochemical immunosensor using polyaniline functionalized graphene quantum dots for detecting a depression marker

    DOI: 10.1016/j.msec.2020.110797

    PMID: 32279828

    Sample: Serum
  4. ACS Omega (2022) IF: 3.512
    Thymoquinone Induces Nrf2 Mediated Adaptive Homeostasis: Implication for Mercuric Chloride-Induced Nephrotoxicity

    DOI: 10.1021/acsomega.2c00028

    PMID: 35252727

    Sample: liver
  5. Annals of Translational Medicine (2022) IF: 3.932
    Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulating HIF-1α and VEGF

    DOI: 10.21037/atm-21-6812

    Sample: adipose-derived stem cell (ASCs)
Show AllShorten


  • Show all
  • Reviews
  • Q&A

S**aSubmitted [ May 15 2019 ]

Asked: How can i dilute concentrated biotinylated detection Ab (100x) 14ml with biotinylated detection Ab diluent 120 microlitre?



adminSubmitted [ May 15 2019 ]

Answered: At first, the volume you mentioned is wrong for the two reagents, we provide 14mL biotinylated detection Ab diluent and 120 μL concentrated biotinylated detection Ab (100x) . The method to prepare biotinylated detection Ab working soltion: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100× Concentrated Biotinylated Detection Ab with Biotinylated Detection Ab Diluent at 1:99( eg. Mix 10μL concentrated biotinylated detection Ab(100x) and 990μL biotinylated detection Ab diluent to make 1mL biotinylated detection Ab working soltion).

... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
*When will you use it?