Human IL-1β(Interleukin 1 Beta) ELISA Kit

    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-H0149

      Synonyms:IL1B, IL1-BETA, IL1F2, catabolin

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Human

      Detection Range: 7.81~500 pg/mL

      Sensitivity: 4.69 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Human IL-1β concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-1β. You can calculate the concentration of Human IL-1β in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Human
      Detection Method Colormetric
      Detection Range 7.81—500 pg/mL
      Sensitivity 4.69 pg/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes Human IL-1β in samples. No significant cross-reactivity or interference between Human IL-1β and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration
      (pg/mL)
      O.D Average Corrected
      500 2.387
      2.403
      2.395 2.318
      250 1.684
      1.706
      1.695 1.618
      125 0.956
      0.932
      0.944 0.867
      62.5 0.532
      0.538
      0.535 0.458
      31.25 0.299
      0.279
      0.289 0.212
      15.63 0.192
      0.168
      0.18 0.103
      7.81 0.123
      0.137
      0.13 0.053
      0 0.068
      0.086
      0.077 --

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-1β were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-1β were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 27.70 46.20 197.60 29.10 47.80 192.60
      Standard deviation 1.40 2.60 9.50 1.70 2.80 6.00
      C V (%) 5.05 5.63 4.81 5.84 5.86 3.12

      Recovery

      The recovery of Human IL-1β spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 94-110 100
      EDTA plasma (n=5) 89-102 95
      Cell culture media (n=5) 84-98 91

      Linearity

      Samples were spiked with high concentrations of Human IL-1β and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 95-109 88-100 94-108
      Average (%) 102 94 102
      1:4 Range (%) 95-109 82-94 93-105
      Average (%) 100 88 99
      1:8 Range (%) 98-110 82-95 97-109
      Average (%) 105 88 103
      1:16 Range (%) 96-108 80-91 97-113
      Average (%) 102 86 103

      Kit components & Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 4℃(shading light)
      Stop Solution 1 vial, 10 mL 4℃
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37℃
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1.Add 100 μL standard or sample to each well. Incubate for 90 min at 37℃.

      elisa assay procedure 2

      2.Remove the liquid.

      elisa assay procedure 3

      3.Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37℃. Aspirate and wash 3 times.

      elisa assay procedure 4

      4.Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

      elisa assay procedure 5

      5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃.

      elisa assay procedure 6

      6.Add 50 μL Stop Solution.

      elisa assay procedure 7

      7.Read at 450 nm immediately. Calculation of results.

      Citations

      1. Publication: Tian P, Li B, He C, et al. Antidiabetic (Type 2) Effects Of Lactobacillus G15 And Q14 In Rats Through Regulation Of Intestinal Permeability And Microbiota[J]. Food & Function, 2016.
        Sample Type: Plasma and Serum
      2. Publication: Dwaich K H, Alamran F G, Alsheibani B I, et al. Melatonin Effects On Myocardial Ischemia-Reperfusion Injury: Impact On The Outcome In Patients Undergoing Coronary Artery Bypass Grafting Surgery[J]. International Journal of Cardiology, 2016, 221: 977-986.
        Sample Type: Plasma
      3. Publication: Veena V K, Popavath R N, Kennedy K, et al. In Vitro Antiproliferative, Pro-Apoptotic, Antimetastatic And Anti-Inflammatory Potential Of 2, 4-Diacteylphloroglucinol (DAPG) By Pseudomonas Aeruginosa Strain FP10[J]. Apoptosis, 2015, 20(10): 1281-1295.
        Sample Type: culture supernatant
      4. Publication: Jiang Y, Kou J, Han X, et al. Ros-Dependent Activation Of Autophagy Through The PI3K/Akt/mTOR Pathway Is Induced By Hydroxysafflor Yellow A-Sonodynamic Therapy In THP-1 Macrophages[J]. Oxidative Medicine and Cellular Longevity, 2017.
        Sample Type: culture supernatant
      5. Publication: Isaza-Guzmán D M, Medina-Piedrahíta V M, Gutiérrez-Henao C, et al. Salivary Levels Of NLRP3 Inflammasome-Related Proteins As Potential Biomarkers Of Periodontal Clinical Status[J]. Journal of Periodontology, 2017.
        Sample Type: Saliva
      6. Publication: Cao Z, Chen L. Inhibition Of miR‑27a Suppresses The Inflammatory Response Via The p38/MAPK Pathway In Intervertebral Disc Cells[J]. Experimental and Therapeutic Medicine, 2017, 14(5): 4572-4578.
        Sample Type: culture supernatant
      7. Publication: Pan Y Y, Zhou F H, Song Z H, et al. Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1-7) upregulation[J]. Biomedicine & Pharmacotherapy, 2018, 97: 1694-1700.
        Sample Type: Serum and culture supernatant
      8. Publication: Lu A Q, Wang Z R, Wang S C. Role Of miR-589-3p In Human Lumbar Disc Degeneration And Its Potential Mechanism[J]. Experimental and Therapeutic Medicine, 2018, 15: 1616-1621.
        Sample Type: culture supernatant
      9. Publication: Jiang Z, Wang W H, Guo C C. Tetrahydroxy Stilbene Glucoside Ameliorates H2O2-Induced Human Brain Microvascular Endothelial Cell Dysfunction In Vitro By Inhibiting Oxidative Stress And Inflammatory Responses[J]. Molecular Medicine Reports, 2017, 16(4): 5219-5224.
        Sample Type: culture supernatant
      10. Publication: Yu H, Dong R, Lu Y Y, et al. Short-Term Postoperative Cognitive Dysfunction And Inflammatory Response In Patients Undergoing Cytoreductive Surgery And Hyperthermic Intraperitoneal Chemotherapy: A Pilot Study[J]. Mediators Of Inflammation, 2017.
        Sample Type: Serum
      11. Publication: Dwaich K H, Al-Amran F G Y, Al-Sheibani B I M, et al. Melatonin Effects On Myocardial Ischemia-Reperfusion Injury: Impact On The Outcome In Patients Undergoing Coronary Artery Bypass Grafting Surgery[J]. International Journal of Cardiology, 2016, 221: 977-986.
        Sample Type: Plasma
      12. Publication: Zhou P, Liu H, Wu Y, et al. Propofol Promotes Ankle Fracture Healing in Children by Inhibiting Inflammatory Response[J]. Medical Science Monitor: International Medical Journal of Experimental And Clinical Research, 2018, 24: 4379.
        Sample Type: Human
      13. Publication: Jasim N A, Kamal Y M, Mohammed M M, et al. 14. Evaluation of the Anti-Inflammatory Effect of Telmisartan As an Adjuvant Therapy to NSAID in The Management of Knee Osteoarthritis; A Clinical Prospective Study[J]. Iraqi JMS 2018; Vol. 16(1): 100-110.
        Sample Type: Human
      14. Publication: Wang X , Gao Y , Song J , et al. The TIR/BB‐loop mimetic AS‐1 prevents non‐alcoholic steatohepatitis and hepatic insulin resistance by inhibiting NLRP3‐ASC inflammasome activation[J]. Br J Pharmacol, 2017, 174(12):1841.
        Sample Type: Mouse
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