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Human IL-1β(Interleukin 1 Beta) ELISA Kit

Citations(74)Uniprot : P01584

  • Cat.No.:E-EL-H0149

  • Reactivity: Human
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Manual MSDS

To Purchase E-EL-H0149

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 7.81-500 pg/mL
Sensitivity 4.69 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-1β in samples. No significant cross-reactivity or interference between Human IL-1β and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-1β concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-1β. You can calculate the concentration of Human IL-1β in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-1β were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-1β were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 27.70 46.20 197.60 29.10 47.80 192.60
Standard deviation 1.40 2.60 9.50 1.70 2.80 6.00
CV (%) 5.05 5.63 4.81 5.84 5.86 3.12

Recovery

The recovery of Human IL-1β spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-110 100
EDTA plasma (n=8) 89-102 95
Cell culture media (n=8) 84-98 91

Linearity

Samples were spiked with high concentrations of Human IL-1β and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P01584
SynonymsIL1B, IL1-BETA, IL1F2, catabolin
Research AreaCancer, Cardiovascular, Metabolism, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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  1. Cell (2020) IF: 38.637
    Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output

    DOI: 10.1016/j.cell.2020.08.009
    Sample: Cell culture medium
  2. Hepatology (2020) IF: 14.679
    Increased Liver Localization of Lipopolysaccharides in Human and Experimental NAFLD

    DOI: 10.1002/hep.31056

    PMID: 31808577

    		 Sample: Serum
  3. Cell Reports (2020) IF: 8.109
    Macrophage K63-Linked Ubiquitination of YAP Promotes Its Nuclear Localization and Exacerbates Atherosclerosis

    DOI: 10.1016/j.celrep.2020.107990

    PMID: 32755583

    		 Sample: Plasma
  4. Carbohydrate Polymers (2019) IF: 7.182
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    DOI: 10.1016/j.carbpol.2020.116163

    PMID: 32241426

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  5. Cancers (2019) IF: 6.126
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    DOI: 10.3390/cancers11122038

    PMID: 31861116

    		 Sample: Serum,Plasma,Whole Blood,Platelet
  6. Diabetologia (2019) IF: 6.023
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    DOI: 10.1007/s00125-018-4755-3

    PMID: 30411254

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  7. EBioMedicine (2018) IF: 6.183
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    DOI: 10.1016/j.ebiom.2018.11.015

    PMID: 30448228

    		 Sample: Cell culture supernatant
  8. International Journal of Cardiology (2016) IF: 6.189
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    DOI: 10.1016/j.ijcard.2016.07.108

    PMID: 27441478

    		 Sample: Plasma
  9. The FASEB Journal (2020) IF: 4.966
    Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output

    DOI: 10.1016/j.cell.2020.08.009

    PMID: 32931733

    		 Sample: Cell culture supernatant
  10. Aging (Albany NY) (2020) IF: 4.831
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    DOI: 10.18632/aging.102710

    PMID: 32003754

    		 Sample: Cell culture supernatant
  11. Frontiers in Pharmacology (2020) IF: 4.225
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    PMID: 32063848

    		 Sample: Cell culture supernatant
  12. Biomedicine & Pharmacotherapy (2020) IF: 4.545
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    DOI: 10.1016/j.biopha.2020.110016

    PMID: 32145584

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    DOI: 10.1016/j.biopha.2020.110326
    Sample: Cell culture supernatant
  14. European Journal of Internal Medicine (2020) IF: 4.329
    Treating periodontal disease in patients with myocardial infarction: A randomized clinical trial

    DOI: 10.1016/j.ejim.2019.08.012

    PMID: 31810741

    		 Sample: Serum
  15. Clinical & Experimental Allergy (2020) IF: 4.217
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    DOI: 10.1111/cea.13570

    PMID: 31955476

    		 Sample: Plasma
  16. Oxidative Medicine and Cellular Longevity (2017) IF: 4.593
    Ros-Dependent Activation Of Autophagy Through The PI3K/Akt/mTOR Pathway Is Induced By Hydroxysafflor Yellow A-Sonodynamic Therapy In THP-1 Macrophages.

    DOI: 10.1155/2017/8519169

    PMID: 28191279

    		 Sample: Cell culture supernatant
  17. Scientific reports (2020) IF: 3.998
    Early modulation of macrophage ROS-PPARγ-NF-κB signalling by sonodynamic therapy attenuates neointimal hyperplasia in rabbits

    DOI: 10.1038/s41598-020-68543-9

    PMID: 32669704

    		 Sample: Cell culture supernatant
  18. International Immunopharmacology (2020) IF: 3.943
    IL-38 alleviates the inflammatory response and the degeneration of nucleus pulposus cells via inhibition of the NF-κB signaling pathway in vitro

    DOI: 10.1016/j.intimp.2020.106592

    PMID: 32502922

    		 Sample: Cell culture supernatant
  19. Fish & Shellfish Immunology (2020) IF: 3.298
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    DOI: 10.1016/j.fsi.2020.06.024

    PMID: 32585358

    		 Sample: Cell culture supernatant
  20. European Journal of Pharmacology (2020) IF: 3.263
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    DOI: 10.1016/j.yexcr.2020.112086

    PMID: 32416091

    		 Sample: Serum,Cell sample
  21. Inflammation (2020) IF: 3.212
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    DOI: 10.1007/s10753-020-01346-2

    PMID: 33009637

    		 Sample: Cell culture supernatant
  22. Journal of Ethnopharmacology (2020) IF: 3.69
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    DOI: 10.1016/j.jep.2020.113039

    PMID: 32497675

    		 Sample: Serum,Cell culture supernatant
  23. Neurochemical Research (2020) IF: 3.038
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    DOI: 10.1007/s11064-020-02991-7

    PMID: 32124161

    		 Sample: Cell culture medium
  24. European review for medical and pharmacological sciences (2020) IF: 3.024
    miR-942-5p alleviates septic acute kidney injury by targeting FOXO3

    DOI: 10.26355/eurrev_202006_21521

    PMID: 32572890

    		 Sample: Cell culture supernatant
  25. Journal of gastroenterology and hepatology (2020) IF: 3.437
    Role of aldosterone in the activation of primary mice hepatic stellate cell and liver fibrosis via NLRP3 inflammasome

    DOI: 10.1111/jgh.14961

    PMID: 31860730

    		 Sample: Serum,Tissue homogenate
  26. Journal of Ethnopharmacology (2020) IF: 3.69
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    DOI: 10.1016/j.jep.2019.112433

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    		 Sample: Cell culture supernatant
  27. Inflammopharmacology (2020) IF: 3.238
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    DOI: 10.1007/s10787-019-00628-y

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    		 Sample: Serum
  28. Inflammation (2020) IF: 3.212
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    DOI: 10.1007/s10753-019-01151-6

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  29. Fish & Shellfish Immunology (2019) IF: 3.298
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    DOI: 10.1016/j.fsi.2019.08.008

    PMID: 31394160

    		 Sample: Cell culture supernatant
  30. International immunopharmacology (2019) IF: 3.118
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    DOI: 10.1016/j.intimp.2018.11.032

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    DOI: 10.1016/j.lfs.2018.12.020

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  32. Molecular Immunology (2019) IF: 3.188
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    DOI: 10.1016/j.molimm.2019.03.011

    PMID: 30952009

    		 Sample: Plasma,Cell culture supernatant,Bronchoalveolar La
  33. ASSESSMENT (2018) IF: 3.197
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    DOI: 10.22159/ajpcr.2018.v11i10.28936
    Sample: Serum
  34. Biomedicine & Pharmacotherapy (2019) IF: 3.457
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    DOI: 10.1016/j.biopha.2019.108706

    PMID: 30844656

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  35. Journal of Ethnopharmacology (2019) IF: 3.414
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    DOI: 10.1016/j.jep.2019.112361

    PMID: 31683033

    		 Sample: Cell culture supernatant
  36. Mediators Of Inflammation (2017) IF: 3.232
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    DOI: 10.1155/2017/3605350

    PMID: 29249869

    		 Sample: Serum
  37. Journal of Periodontology (2017) IF: 3.03
    Salivary Levels Of NLRP3 Inflammasome-Related Proteins As Potential Biomarkers Of Periodontal Clinical Status.

    DOI: 10.1902/jop.2017.170244

    PMID: 28691886

    		 Sample: Saliva
  38. Apoptosis (2015) IF: 3.833
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    DOI: 10.1007/s10495-015-1162-9

    PMID: 26283170

    		 Sample: Cell culture supernatant
  39. Food & Function (2016) IF: 3.247
    Antidiabetic (Type 2) Effects Of Lactobacillus G15 And Q14 In Rats Through Regulation Of Intestinal Permeability And Microbiota.

    DOI: 10.1039/c6fo00831c

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    		 Sample: Plasma,Serum
  40. Medical Archives (2020) IF: 2.807
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    DOI: 10.5455/medarh.2020.74.199-204

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  41. BioMed Research International (2020) IF: 2.276
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    DOI: 10.1155/2020/5341247

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  42. Human & Experimental Toxicology (2020) IF: 2.067
    Investigation of DNA damage, oxidative stress, and inflammation in synthetic cannabinoid users

    DOI: 10.1177/0960327120930057

    PMID: 32508150

    		 Sample: Plasma
  43. Archives of Physiology and Biochemistry (2020) IF: 2.575
    XIST silencing alleviated inflammation and mesangial cells proliferation in diabetic nephropathy by sponging miR-485

    DOI: 10.1080/13813455.2020.1789880

    PMID: 32669002

    		 Sample: Serum
  44. Immunopharmacology and Immunotoxicology (2020) IF: 2.352
    A case of asthma with Beh?et's disease: successful treatment with omalizumab and its effects on recurrent aphthous lesions

    DOI: 10.1080/08923973.2020.1789656

    PMID: 32605504

    		 Sample: Serum
  45. Biological Trace Element Research (2019) IF: 2.639
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    DOI: 10.1007/s12011-019-01831-2

    PMID: 31473895

    		 Sample: Cell lysate
  46. Biochemical and Biophysical Research Communications (2020) IF: 2.985
    Long non-coding RNA MEG3 regulates CSE-induced apoptosis and inflammation via regulating miR-218 in 16HBE cells

    DOI: 10.1016/j.bbrc.2019.10.135

    PMID: 31668807

    		 Sample: Cell culture supernatant
  47. Bioscience reports (2019) IF: 2.942
    Up-regulated MCPIP1 in abdominal aortic aneurysm is associated with vascular smooth muscle cell apoptosis and MMPs production

    DOI: 10.1042/BSR20191252

    PMID: 31651935

    		 Sample: Serum,Plasma,Cell sample
  48. Biochemical and biophysical research communications (2019) IF: 2.559
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    DOI: 10.1016/j.bbrc.2018.11.189

    PMID: 30528233

    		 Sample: Serum
  49. Evidence-Based Complementary and Alternative Medicine (2019) IF: 2.064
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    DOI: 10.1155/2019/5961742

    PMID: 30941196

    		 Sample: Cell culture supernatant
  50. Molecular and cellular biochemistry (2019) IF: 2.561
    Exogenous hydrogen sulfide mitigates LPS+ ATP-induced inflammation by inhibiting NLRP3 inflammasome activation and promoting autophagy in L02 cells

    DOI: 10.1007/s11010-019-03519-6

    PMID: 30877511

    		 Sample: Cell culture supernatant
  51. Biomedicine & Pharmacotherapy (2018) IF: 2.759
    Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1-7) upregulation.

    DOI: 10.1016/j.biopha.2017.11.151

    PMID: 29793333

    		 Sample: Cell culture supernatant,Serum
  52. Archives of Oral Biology (2020) IF: 1.931
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    PMID: 32805637

    		 Sample: Cell culture supernatant
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    DOI: 10.12659/MSM.921957

    PMID: 32463805

    		 Sample: Cell culture supernatant
  54. Archives of Oral Biology (2019) IF: 1.931
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    DOI: 10.1016/j.archoralbio.2019.104514

    PMID: 31394382

    		 Sample: Cell culture supernatant
  55. Journal of Clinical Neuroscience (2019) IF: 1.76
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    DOI: 10.1016/j.jocn.2019.10.002

    PMID: 31629608

    		 Sample: Serum
  56. Genetic testing and molecular biomarkers (2019) IF: 1.181
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    DOI: 10.1089/gtmb.2018.0219

    PMID: 30688539

    		 Sample: Synovia
  57. Experimental and therapeutic medicine (2018) IF: 1.41
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    DOI: 10.3892/etm.2018.6721

    PMID: 30402165

    		 Sample: Serum
  58. Molecular Medicine Reports (2017) IF: 1.692
    Tetrahydroxy Stilbene Glucoside Ameliorates H2O2-Induced Human Brain Microvascular Endothelial Cell Dysfunction In Vitro By Inhibiting Oxidative Stress And Inflammatory Responses.

    DOI: 10.3892/mmr.2017.7225

    PMID: 28849141

    		 Sample: Cell culture supernatant
  59. Experimental and Therapeutic Medicine (2018) IF: 1.261
    Role Of miR-589-3p In Human Lumbar Disc Degeneration And Its Potential Mechanism.

    DOI: 10.3892/etm.2017.5593

    PMID: 29434746

    		 Sample: Cell culture supernatant
  60. Experimental and Therapeutic Medicine (2017) IF: 1.261
    Inhibition Of miR27a Suppresses The Inflammatory Response Via The p38/MAPK Pathway In Intervertebral Disc Cells.

    DOI: 10.3892/etm.2017.5053

    PMID: 29067127

    		 Sample: Cell culture supernatant
  61. Preprint (2019)
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    DOI: 10.20944/preprints201905.0285.v1
    Sample: Serum
  62. Plant Archives (2020)
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    Sample: Serum
  63. Indian Journal of Public Health Research & Development (2019)
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    DOI: 10.5958/0976-5506.2019.01645.0
    Sample: Serum
  64. The Journal of Research on the Lepidoptera (2020)
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    DOI: 10.36872/LEPI/V51I2/301090
    Sample: Serum
  65. Preprints (2020)
    Lowered serum cesium levels in schizophrenia: association with immune-inflammatory biomarkers and cognitive impairments

    DOI: 10.1590/1516-4446-2020-0908

    PMID: 32556004

    		 Sample: Serum
  66. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE (2019) IF: 0.166
    Resveratrol inhibits IL-1 beta, IL-18, and ICAM-1 in a nasopharyngeal carcinoma model
    Sample: Serum,Tissue homogenate
  67. Journal of biomedical research (2017)
    Clinical application of simultaneous detection of cystatin C, cathepsin S, and IL-1 in classification of coronary artery disease

    DOI: 10.7555/JBR.31.20150152

    PMID: 28808203

    		 Sample: Serum
  68. NTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE (2019) IF: 0.181
    Resveratrol protects human renal proximal tubular cells from high glucose and hypoxia/reoxygenation induced injury via inhibiting p38-MAPK and thioredoxin-interacting protein (TXNIP) pathways
    Sample: Cell culture supernatant
  69. Journal of Pharmaceutical Sciences and Research (2018)
    Effect of General and Spinal Anesthesia on Some Anti-inflammatory cytokines and cell adhesion molecules of Iraqi Women Undergo Cesarean Section: Comparative Study
    Sample: Serum
  70. Medical science monitor: international medical journal of experimental and clinical research (2018)
    Sinomenine Regulates Inflammatory Response and Oxidative Stress via Nuclear Factor kappa B (NF-kB) and NF-E2-Related Factor 2 (Nrf2) Signaling Pathways in Ankle Fractures in Children

    DOI: 10.12659/MSM.910740

    PMID: 30237391

    		 Sample: Serum
  71. Iraqi Journal of Science (2018)
    Estimation of Levels of Interleukin-1 beta and Interleukin-10 in Sera of some Iraqi Patients with Chronic Rheumatoid Arthritis
    Sample: Serum
  72. Indian Journal of Public Health Research & Development (2019)
    The Role of Serum and Follicular IL-1Beta in Predicting the ICSI Outcome in Infertile Women.

    DOI: 10.5958/0976-5506.2019.00100.1
    Sample: Serum
  73. Medical Science Monitor: International Medical Journal of Experimental And Clinical Research (2018) IF: /
    Propofol Promotes Ankle Fracture Healing in Children by Inhibiting Inflammatory Response.

    DOI: 10.12659/MSM.908592

    PMID: 29940605

    		 Sample: Serum
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