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Human IL-1β(Interleukin 1 Beta) ELISA Kit

  • Cat.No.:E-EL-H0149

  • Reactivity: Human

To Purchase E-EL-H0149

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 7.81-500 pg/mL
Sensitivity 4.69 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-1β in samples. No significant cross-reactivity or interference between Human IL-1β and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-1β concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-1β. You can calculate the concentration of Human IL-1β in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-1β were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-1β were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 27.70 46.20 197.60 29.10 47.80 192.60
Standard deviation 1.40 2.60 9.50 1.70 2.80 6.00
CV (%) 5.05 5.63 4.81 5.84 5.86 3.12

Recovery

The recovery of Human IL-1β spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-110 100
EDTA plasma (n=8) 89-102 95
Cell culture media (n=8) 84-98 91

Linearity

Samples were spiked with high concentrations of Human IL-1β and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P01584
SynonymsIL1B, IL1-BETA, IL1F2, catabolin
Research AreaCancer,Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Cell (2020) IF: 38.637
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    DOI: 10.1016/j.cell.2020.08.009

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  2. Hepatology (2020) IF: 14.679
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    DOI: 10.1002/hep.31056

    PMID: 31808577

    Sample: Serum
  3. Cell Reports (2020) IF: 8.109
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    DOI: 10.1016/j.celrep.2020.107990

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    Sample: Plasma
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    DOI: 10.1016/j.carbpol.2020.116163

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    Sample: Gingival Crevicular Fluid
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    DOI: 10.3390/cancers11122038

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    Sample: Serum,Plasma,Whole Blood,Platelet
  6. Diabetologia (2019) IF: 6.023
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    DOI: 10.1007/s00125-018-4755-3

    PMID: 30411254

    Sample: Cell culture supernatant
  7. EBioMedicine (2018) IF: 6.183
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    DOI: 10.1016/j.ebiom.2018.11.015

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    Sample: Cell culture supernatant
  8. British Journal of Pharmacology (2017) IF: 6.81
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    DOI: 10.1111/bph.13786

    PMID: 28306139

    Sample: Cell culture supernatant
  9. International Journal of Cardiology (2016) IF: 6.189
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    DOI: 10.1016/j.ijcard.2016.07.108

    PMID: 27441478

    Sample: Plasma
  10. International Journal of Cardiology (2016) IF: 6.189
    Melatonin Effects On Myocardial Ischemia-Reperfusion Injury: Impact On The Outcome In Patients Undergoing Coronary Artery Bypass Grafting Surgery.

    DOI: 10.1016/j.ijcard.2016.07.108

    PMID: 27441478

    Sample: Plasma
  11. The FASEB Journal (2020) IF: 4.966
    Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output

    DOI: 10.1016/j.cell.2020.08.009

    PMID: 32931733

    Sample: Cell culture supernatant
  12. Aging (Albany NY) (2020) IF: 4.831
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    DOI: 10.18632/aging.102710

    PMID: 32003754

    Sample: Cell culture supernatant
  13. Frontiers in Pharmacology (2020) IF: 4.225
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  14. Biomedicine & Pharmacotherapy (2020) IF: 4.545
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    DOI: 10.1016/j.biopha.2020.110016

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  15. Biomedicine & Pharmacotherapy (2020) IF: 4.545
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    DOI: 10.1016/j.biopha.2020.110326

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    Sample: Cell culture supernatant
  16. European Journal of Internal Medicine (2020) IF: 4.329
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    DOI: 10.1016/j.ejim.2019.08.012

    PMID: 31810741

    Sample: Serum
  17. Clinical & Experimental Allergy (2020) IF: 4.217
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    DOI: 10.1111/cea.13570

    PMID: 31955476

    Sample: Plasma
  18. Oxidative Medicine and Cellular Longevity (2017) IF: 4.593
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    DOI: 10.1155/2017/8519169

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    Sample: Cell culture supernatant
  19. Scientific reports (2020) IF: 3.998
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    DOI: 10.1038/s41598-020-68543-9

    PMID: 32669704

    Sample: Cell culture supernatant
  20. International Immunopharmacology (2020) IF: 3.943
    IL-38 alleviates the inflammatory response and the degeneration of nucleus pulposus cells via inhibition of the NF-κB signaling pathway in vitro

    DOI: 10.1016/j.intimp.2020.106592

    PMID: 32502922

    Sample: Cell culture supernatant
  21. Fish & Shellfish Immunology (2020) IF: 3.298
    Molecular characterization, inducible expression and functional analysis of an IKKβ from the tropical sea cucumber Holothuria leucospilota

    DOI: 10.1016/j.fsi.2020.06.024

    PMID: 32585358

    Sample: Cell culture supernatant
  22. European Journal of Pharmacology (2020) IF: 3.263
    iTRAQ-based proteomics and in vitro experiments reveals essential roles of ACE and AP-N in the renin-angiotensin system-mediated congenital ureteropelvic junction obstruction

    DOI: 10.1016/j.yexcr.2020.112086

    PMID: 32416091

    Sample: Serum,Cell sample
  23. Inflammation (2020) IF: 3.212
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    DOI: 10.1007/s10753-020-01346-2

    PMID: 33009637

    Sample: Cell culture supernatant
  24. Journal of Ethnopharmacology (2020) IF: 3.69
    Anti-angiogenic effect of Shikonin in rheumatoid arthritis by downregulating PI3K/AKT and MAPKs signaling pathways

    DOI: 10.1016/j.jep.2020.113039

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    Sample: Serum,Cell culture supernatant
  25. Neurochemical Research (2020) IF: 3.038
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  26. European review for medical and pharmacological sciences (2020) IF: 3.024
    miR-942-5p alleviates septic acute kidney injury by targeting FOXO3

    DOI: 10.26355/eurrev_202006_21521

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    Sample: Cell culture supernatant
  27. Journal of gastroenterology and hepatology (2020) IF: 3.437
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    DOI: 10.1111/jgh.14961

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    Sample: Serum,Tissue homogenate
  28. Journal of Ethnopharmacology (2020) IF: 3.69
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    DOI: 10.1016/j.jep.2019.112433

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    Sample: Cell culture supernatant
  29. Inflammopharmacology (2020) IF: 3.238
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  30. Inflammation (2020) IF: 3.212
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    DOI: 10.1007/s10753-019-01151-6

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  31. Fish & Shellfish Immunology (2019) IF: 3.298
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    DOI: 10.1016/j.fsi.2019.08.008

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  32. International immunopharmacology (2019) IF: 3.118
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    DOI: 10.1016/j.intimp.2018.11.032

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  33. Life sciences (2019) IF: 3.234
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    DOI: 10.1016/j.lfs.2018.12.020

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  34. Molecular Immunology (2019) IF: 3.188
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    DOI: 10.1016/j.molimm.2019.03.011

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  35. ASSESSMENT (2018) IF: 3.197
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  38. Mediators Of Inflammation (2017) IF: 3.232
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    DOI: 10.1155/2017/3605350

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    DOI: 10.1902/jop.2017.170244

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    DOI: 10.1007/s10495-015-1162-9

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  42. Medical Archives (2020) IF: 2.807
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    DOI: 10.5455/medarh.2020.74.199-204

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  44. Human & Experimental Toxicology (2020) IF: 2.067
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  45. Archives of Physiology and Biochemistry (2020) IF: 2.575
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    DOI: 10.1080/13813455.2020.1789880

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    Sample: Serum
  46. Immunopharmacology and Immunotoxicology (2020) IF: 2.352
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    DOI: 10.1080/08923973.2020.1789656

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    Sample: Serum
  47. Biological Trace Element Research (2019) IF: 2.639
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    DOI: 10.1007/s12011-019-01831-2

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    Sample: Cell lysate
  48. Biochemical and Biophysical Research Communications (2020) IF: 2.985
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    DOI: 10.1016/j.bbrc.2019.10.135

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    Sample: Cell culture supernatant
  49. Bioscience reports (2019) IF: 2.942
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    DOI: 10.1042/BSR20191252

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    Sample: Serum,Plasma,Cell sample
  50. Biochemical and biophysical research communications (2019) IF: 2.559
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    Sample: Serum
  51. Evidence-Based Complementary and Alternative Medicine (2019) IF: 2.064
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  52. Molecular and cellular biochemistry (2019) IF: 2.561
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    DOI: 10.1007/s11010-019-03519-6

    PMID: 30877511

    Sample: Cell culture supernatant
  53. Biomedicine & Pharmacotherapy (2018) IF: 2.759
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    DOI: 10.1016/j.biopha.2017.11.151

    PMID: 29793333

    Sample: Cell culture supernatant,Serum
  54. Archives of Oral Biology (2020) IF: 1.931
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    Sample: Cell culture supernatant
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    Sample: Cell culture supernatant
  56. Archives of Oral Biology (2019) IF: 1.931
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    DOI: 10.1016/j.archoralbio.2019.104514

    PMID: 31394382

    Sample: Cell culture supernatant
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    DOI: 10.1016/j.jocn.2019.10.002

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    Sample: Serum
  58. Genetic testing and molecular biomarkers (2019) IF: 1.181
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  59. Experimental and therapeutic medicine (2018) IF: 1.41
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    DOI: 10.3892/etm.2018.6721

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    Sample: Serum
  60. Molecular Medicine Reports (2017) IF: 1.692
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    DOI: 10.3892/mmr.2017.7225

    PMID: 28849141

    Sample: Cell culture supernatant
  61. Experimental and Therapeutic Medicine (2018) IF: 1.261
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    DOI: 10.3892/etm.2017.5593

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    Sample: Cell culture supernatant
  62. Experimental and Therapeutic Medicine (2017) IF: 1.261
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    DOI: 10.3892/etm.2017.5053

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    Sample: Cell culture supernatant
  63. Preprint (2019)
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    DOI: 10.20944/preprints201905.0285.v1

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    Sample: Serum
  64. Plant Archives (2020)
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    DOI: 99999999

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    Sample: Serum
  65. Indian Journal of Public Health Research & Development (2019)
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    DOI: 10.5958/0976-5506.2019.01645.0

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    Sample: Serum
  66. The Journal of Research on the Lepidoptera (2020)
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    DOI: 10.36872/LEPI/V51I2/301090

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    Sample: Serum
  67. Preprints (2020)
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    DOI: 10.1590/1516-4446-2020-0908

    PMID: 32556004

    Sample: Serum
  68. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE (2019) IF: 0.166
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    DOI: 99999999

    PMID: 99999999

    Sample: Serum,Tissue homogenate
  69. Journal of biomedical research (2017)
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    DOI: 10.7555/JBR.31.20150152

    PMID: 28808203

    Sample: Serum
  70. NTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE (2019) IF: 0.181
    Resveratrol protects human renal proximal tubular cells from high glucose and hypoxia/reoxygenation induced injury via inhibiting p38-MAPK and thioredoxin-interacting protein (TXNIP) pathways

    DOI: 99999999

    PMID: 99999999

    Sample: Cell culture supernatant
  71. Journal of Pharmaceutical Sciences and Research (2018)
    Effect of General and Spinal Anesthesia on Some Anti-inflammatory cytokines and cell adhesion molecules of Iraqi Women Undergo Cesarean Section: Comparative Study

    DOI: 99999999

    PMID: 99999999

    Sample: Serum
  72. Medical science monitor: international medical journal of experimental and clinical research (2018)
    Sinomenine Regulates Inflammatory Response and Oxidative Stress via Nuclear Factor kappa B (NF-kB) and NF-E2-Related Factor 2 (Nrf2) Signaling Pathways in Ankle Fractures in Children

    DOI: 10.12659/MSM.910740

    PMID: 30237391

    Sample: Serum
  73. Iraqi Journal of Science (2018)
    Estimation of Levels of Interleukin-1 beta and Interleukin-10 in Sera of some Iraqi Patients with Chronic Rheumatoid Arthritis

    DOI: 99999999

    PMID: 99999999

    Sample: Serum
  74. Indian Journal of Public Health Research & Development (2019)
    The Role of Serum and Follicular IL-1Beta in Predicting the ICSI Outcome in Infertile Women.

    DOI: 10.5958/0976-5506.2019.00100.1

    PMID: 99999999

    Sample: Serum
  75. Iraqi JMS (2018) IF: /
    Evaluation of the Anti-Inflammatory Effect of Telmisartan As an Adjuvant Therapy to NSAID in The Management of Knee Osteoarthritis; A Clinical Prospective Study.

    DOI: 10.22578/IJMS.16.1.14

    Sample: Serum
  76. Medical Science Monitor: International Medical Journal of Experimental And Clinical Research (2018) IF: /
    Propofol Promotes Ankle Fracture Healing in Children by Inhibiting Inflammatory Response.

    DOI: 10.12659/MSM.908592

    PMID: 29940605

    Sample: Serum
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