Current Position:Home>>Kits>>ELISA Kits>> Human IL-34(Interleukin 34) ELISA Kit
Cat.No.:E-EL-H1650
Welcome to order from local distributors.
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 93.75-6000 pg/mL |
Sensitivity | 56.25 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human IL-34 in samples. No significant cross-reactivity or interference between Human IL-34 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human IL-34 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-34 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-34 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 292.00 | 719.71 | 2331.63 | 295.64 | 706.23 | 2493.50 |
Standard deviation | 15.07 | 38.22 | 90.47 | 15.46 | 39.62 | 112.71 |
CV (%) | 5.16 | 5.31 | 3.88 | 5.23 | 5.61 | 4.52 |
The recovery of Human IL-34 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 92-102 | 97 |
EDTA plasma (n=8) | 84-97 | 91 |
Cell culture media (n=8) | 92-106 | 98 |
Samples were spiked with high concentrations of Human IL-34 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
Q6ZMJ4
|
Synonyms | IL34, C16orf77, Chromosome 16 Open Reading Frame 77 |
Research Area | Cancer, Immunology |
![]() |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
![]() |
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
![]() |
3. Aspirate and wash the plate for 3 times |
![]() |
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
![]() |
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
![]() |
6. Add 50μL Stop Solution |
![]() |
7. Read the plate at 450nm immediately. Calculation of the results |
From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!
Sample: cerebrospinal fluid (CSF) |